A study on salt tolerance and the genetic stability of T1 generation transgenic alfalfa with the BADH gene

Abstract

Abstract: The physiological response of transgenic alfalfa to different salt stresses and its PCR detection were investigated. The ratio of negative to positive numbers of PCR detection confirmed the first Mendelian law. The transgenenic T1 generations showed enhanced BADH activity and betaine content, compared to the non-transgenenic plants at 0.8% NaCl stress. Proline and soluble sugar contents and the antioxidase activity of transgenic T1 generations were higher than those of non-transgenic plants at different salt stresses, while electrolyte leakage and malonaldehyde contents were lower. This suggests that the BADH gene was expressed in the transgenic T1 generation, resulting in improved salt tolerance in the transgenic plants.

YAN Li-ping, XIA Yang, LIANG Hui-min, WANG Zhen-zhen,
LI Shuang-yun, LIU Cui-lan, PANG Cai-hong. A study on salt tolerance and the genetic stability of T1 generation transgenic alfalfa with the BADH gene[J]. Acta Prataculturae Sinica, 2009, 18(6): 65-71.

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