Identification and genetic variation analysis of orchardgrass hybrids(Dactylis glomerata)by SSR molecular markers

Abstract

Abstract: Hybrids (140) of orchardgrass (Dactylis glomerata)and their parental lines (01996, YA02-103) were tested by simple sequence repeats (SSR) markers. The amplification bands of hybrids showed complementary patterns of both parents, the male pattern and other patterns. Three complementary pattern primers: A01G20, A03N16, A03K22 could effectively and reliably detect 111 hybrids. A total of 76 polymorphic bands were detected by 13 primer pairs, with an average of 6 bands per primer The percentage of polymorphic bands was 84.24%. These results suggested that there was rich genetic variation among all orchardgrass hybrids tested and that the SSR technique has wide prospective use in hybrid identification and genetic analysis of orchardgrass.

CLC Number:

XIE Wen-gang, ZHANG Xin-quan, CHENG Yong-xia. Identification and genetic variation analysis of orchardgrass hybrids(Dactylis glomerata)by SSR molecular markers[J]. Acta Prataculturae Sinica, 2010, 19(2): 212-217.

References

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