Abstract: The morphology, water physiology, and carbon allocation responses of cool-season turfgrass to drought stress and re-watering treatments were studied. Three cultivars (Prize, Nuglade, and Midnight) of Kentucky bluegrass (Poa pratensis), were tested under drought and re-watering treatments to determine the changes in turf quality, leaf relative water content (RWC), total nonstructural carbohydrate (TNC) content and ¹⁴C allocation in different organs. Drought stress caused a significant decline in turf quality and leaf RWC, which varied with cultivar and treatment time. After re-watering, turf quality and leaf RWC recovered to different degrees, especially in Prize. At the early stages during drought stress, ¹⁴C allocation to the roots increased significantly for all cultivars. After 14 d drought stress, ¹⁴C allocation to different organs in the three cultivars was roots＞shoots＞leaves. With prolonged drought stress, some ¹⁴C in the roots moved into the shoots and leaves, resulting in a marked increase of ¹⁴C allocation to the shoots and leaves. After 21 d re-watering, ¹⁴C allocation in the three cultivars was shoots＞leaves＞roots. During the drought stress, TNC accumulation in the roots, shoots, and leaves of the three cultivars continually changed and was in the sequence leaves＞shoots＞roots. After re-watering, the TNC content in the leaves, shoots, and roots of all three cultivars was lower than that under drought stress, but still in the same order. This suggests that the allocation of carbon assimilates and their accumulation in different organs of turfgrasses were a physiological adaptation response to drought and re-watering treatments and provides a scientific basis for further investigating the resistance of turfgrasses to other adverse environments.