Abstract: Embryogenic calli were induced from mature seeds of Nuglade (Poa Pratensis) on MB₅ medium supplemented with 3.0 mg/L 2,4-D, 0.5 mg/L 6-BA. Protoplasts were isolated from the embryogeni calli after subculture for 7-9 months. The protoplasts were cultured on KM₈P medium supplemented with 3.0 mg/L 2,4-D, 0.5 mg/L 6-BA, 100 mg/L casein hydrolysate, 100 mg/L lactablumin hydrolysate , 1% (W/V)sucrose and 0.4 mol/L mannitol. The first divisions happened after 3 days and small cell clusters appeared after 2 to 3 weeks in the culture medium. The protoplast-derived cells could keep dividing sustainably and forming calli by adding fresh protoplast culture liquids once or twice to lower osmotic pressure. The regenerated calli, 3 to 5 mm in diameter, were transferred to solid medium (MS medium supplements with 3.0 mg/L 2,4-D, 0.5 mg/L 6-BA then MS medium supplements with 0.5 mg/L NAA, 5.0 mg/L 6-BA) to carry out subculture for cell propagation, differentiation, then formation of complete plants.