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Acta Prataculturae Sinica ›› 2010, Vol. 19 ›› Issue (4): 79-86.

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A comprehensive evaluation of salt-tolerance and the physiological response of Medicago sativa at the seedling stage

LI Yuan1,2, LIU Gui-bo2, GAO Hong-wen1, SUN Gui-zhi1, ZHAO Hai-ming2, XIE Nan2   

  1. 1.Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China;

    2.Dryland Farming Institute, Hebei Academy of Agricultural and Forestry Sciences Key
    Laboratory for Crop Drought Resistance of Hebei Province, Hengshui 053000, China
  • Received:2009-05-31 Online:2010-08-20 Published:2010-08-20

Abstract: A comprehensive evaluation of salt tolerance and the physiological response of 18 Medicago sativa. germplasms from Russia was studied under different NaCl concentrations (0.3%, 0.4%, 0.5% and 0.6%) in the green house at the seedling stage. They were compared with Zhongmu 1. The standard deviation coefficient allocation weighted method not only considered different indexes allocation weight, but also evaluated drought resistance of all the materials. Four strong salt tolerant germplasms, M7, M9, M15, and 810, were screened using this method. Free proline content, soluble sugar content, relative electrical conductivity, MDA content and water saturation deficit were considerably increased by an increase of salt concentration, while leaf water potential decreased. The soluble sugar content, relative electrical conductivity, MDA content, water saturation deficit and water potential in the leaf at the seedling stage responded sensitively to the level of salt stress and this can be used as an identification index of salt tolerance.

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