Tissue culture and plant regeneration from mature seed of two cultivars of tall fescue

Abstract

Abstract: To obtain a reliable and efficient regeneration system of tall fescue (Festuca arundinacea), mature seeds of ‘SHED’ and ‘Plantation’ were cultured on medium supplemented with combinations of 6-BA and 2,4-D. The optimal induction medium for intact mature seed explants was ‘MS+2,4-D 3 mg/L’, resulting in the highest induction rate of 71%, while that for longitudinally sliced seed explants was ‘MS+2,4-D 2.5 mg/L’ with a highest induction rate of 56%. The optimal subculture medium contained 3 mg/L 2,4-D, giving an embryogenic callus induction rate of 70%, and the addition of 6-BA to this medium adversely affected embryogenic callus induction. The effect of interaction of genotype and plant growth regulator concentration was significant. The highest regeneration rates obtained from the medium ‘MS+2,4-D 1 mg/L+6-BA 1 mg/L’ and ‘MS+2, 4-D 2 mg/L+6-BA 1 mg/L’ were 50% (CV. ‘SHED’) and 56% (CV. ‘Plantation’). The reported rooting medium of ‘1/2 MS+NAA 0.5 mg/L’ gave a rooting ratio of 100%.

CLC Number:

YAN Jun-hui, ZHAO Zhi-yan, HE Ya-li. Tissue culture and plant regeneration from mature seed of two cultivars of tall fescue[J]. Acta Prataculturae Sinica, 2011, 20(2): 210-218.

References

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