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Acta Prataculturae Sinica ›› 2011, Vol. 20 ›› Issue (2): 227-233.

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Genetic structure and variation in different geographical populations of Houttuynia cordata

ZHONG Jun1,3, WANG Kun2, QIU Ping3, ZENG Wei-jun3, Xiong Xing-yao4   

  1. 1.College of Agriculture, Hunan Agricultural University, Changsha 410128, China;
    2.College of Bioscience and technology, Hunan Agricultural University, Changsha 410128, China;
    3.Hunan Zhengqing Pharmaceutical Co.LTD,Huaihua 418000, China;
    4.College of Horticulture and Gardening, Hunan Agricultural University, Changsha 410128, China
  • Received:2010-03-10 Online:2011-02-25 Published:2011-04-20

Abstract: The genetic diversity and genetic structure of Houttuynia cordata populations in Huaihua were analyzed by SRAP (sequence-related amplified polymorphism). The percentages of polymorphy(PPB)of the sixteen populations varied from 53.66% to 85.35% and the average heterozygosities ranged from 0.07 to 0.23,The Shannon index of the total population was 0.10-0.34, which suggested relatively high genetic variability in H. cordata. The genetic differentiation coefficient (Gst) was 0.51 so it is proposed that most of the genetic variation occurred between populations with a certain frequency of gene flow within populations(Nm=0.326). A cluster analysis based on the UPGMA method grouped the majority of the 16 populations at D=0.5 into five main clusters that corresponded to their geographic regions. The results suggested there was a lack of significant association between genetic diversity and environmental factors among the 16 H. cordata populations, although Gst and Nm were enhanced at higher altitudes, where were declining along with raising latitude and longitude.

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