Physiological & biochemical responses of different leaves of Ophiopogon bodinieri to soil lead stress

Abstract

Abstract: Ophiopogon bodinieri is an important turfgrass and ornamental cover plants which is widely used in urban garden construction in southern China. To understand the physiological & biochemical responses of different leaves of O. bodinieri to increasing Pb contamination, the effects of different soil Pb concentrations on the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and on the concentrations of free proline (Pro) and malondialdehyde (MDA) in different leaves were studied in a pot experiment with different Pb treatments. The activities of SOD, POD and CAT and the concentrations of Pro and MDA in fresh leaf (FL), mature leaf (ML), and old leaf (OL) of O. bodinieri were measured after Pb-exposure for 7 days. The activities of SOD, POD, and CAT increased at lower Pb concentrations, but decreased at higher ones, depending on the leaves and enzymes. Meanwhile, Pro and MDA concentrations in FL, ML and OL increased with increased soil Pb. The responses of different leaves of O. bodinieri to soil Pb stress differed. FL had higher activities of the antioxidative enzymes SOD, POD and CAT in comparison with the OL under the levels of soil Pb contamination tested, while the OL had higher concentrations of osmotic substances (Pro and MDA) in comparison with the FL and ML. O. bodinieri could adapt to a certain concentration range of soil Pb stress, and the response of fresh O. bodinieri leaves to soil Pb stress was more sensitive than that of old O. bodinieri leaves. The tolerance of old O. bodinieri leaves to soil Pb stress was stronger than those of fresh and mature leaves.

CLC Number:

LIU Bi-ying, PAN Yuan-zhi, ZHAO Yang-di. Physiological & biochemical responses of different leaves of Ophiopogon bodinieri to soil lead stress[J]. Acta Prataculturae Sinica, 2011, 20(4): 123-128.

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