A rudimentary study of the acid fibroblast growth factor’s plant expression vector construction and transformation into Medicago sativa

Abstract

Abstract: After optimization of the aFGF gene for the chosen plant, the codon, the signal peptide, six-histone tag and zymoplasm cutting factor were added to the modified TΩAB plasmid, which was named TΩAB-aF after the recombination of the aFGF gene and enhancer sequence, promoter, Ω-sequence and ployA tailing sequence double digests. The binary expression vector pBI121-TΩAB-aF was constructed using TΩAB-aF and pBI121. By triparental mating the vector pBI121-TΩAB-aF was transformed into LBA4404. Medicago sativa was transformed using Agrobacterium tumefaciens, followed by selective screening and PCR, RT-PCR and Western Blot analysis. The aFGF was expressed in M. sativa.

CLC Number:

JIANG Shi-cui, WANG Yi, SUN Chun-yu, WANG Yan-fang, LI Xiao-kun, ZHANG Mei-ping. A rudimentary study of the acid fibroblast growth factor’s plant expression vector construction and transformation into Medicago sativa[J]. Acta Prataculturae Sinica, 2011, 20(4): 180-186.

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