Genetic diversity and genetic variation of Ceratoides arborescens wild populations in Inner Mongolia

Abstract

Abstract: By using inter-simple sequence repeat (ISSR) markers technique, this paper studied the genetic diversity and variation of six wild populations of Ceratoides arborescens. The results showed that at species level the percentage of polymorphic bank (PPB) was 98.05%, Nei’s gene diversity (h) and Shannon’s index (I) were 0.298 4 and 0.455 7, respectively. According to PPB, h and I, estimated the trend line of the average genetic diversity within each population was similar, the order was PA>PB>PD>PC>PE>PF. There were more genetic variations within the population than among populations. The gene flow is 4.333 2 among populations, were greater than 1. The cluster analysis suggested that the six populations of C. arborescens could be divided into two groups, i.e. desert grassland group and typical grassland group. The habitat fragmentation was still not enough to cause the loss of specific gene, genetic drift occured very unlikely.

CLC Number:

WANG Pu-chang, ZHAO Li-li, YI Jin, ZHANG Jin-hua. Genetic diversity and genetic variation of Ceratoides arborescens wild populations in Inner Mongolia[J]. Acta Prataculturae Sinica, 2011, 20(4): 287-292.

References

[1] 易津, 王学敏, 乌仁其木格, 等.驼绒藜属牧草种子生理学特性的研究[J]. 种子, 2004, 23(4): 3-9.
[2] 韩智龙, 易津. 华北驼绒藜种子发育过程中内源激素变化动态的研究[J]. 种子, 2008, 29(7): 3-9.
[3] 卢立娜, 李青丰, 贺晓, 等. 华北驼绒藜开花生物学特性研究[J]. 西北植物学报, 2009, 29(6): 1176-1181.
[4] 解新明, 卢小良. SSR和ISSR标记及其在牧草遗传与育种研究中的应用前景[J]. 草业科学, 2005, 22(2): 30-37.
[5] Adams R P, Schwarzbach A E, Pandey R N. The concordance of terpenoid, ISSR and RAPD markers, and its sequence data sets among genotypes: An example from Juniperus[J]. Biochemical Systematics and Ecology, 2003, 31: 375-387.
[6] 花喆斌. 银杏群体遗传多样性的RAPD和ISSR分析[D]. 南京: 南京林业大学, 2007.
[7] Excoffier L, Smouse P E, Ouattro J M, et al. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction sites[J]. Genetics, 1992, 131: 479-491
[8] Hapke A D, Zinner H, Zischler. Mitochondrial DNA variation in Eritrean hamadryas baboons (Papiohamadryas hamadryas): life history influences population genetic structure[J]. Behavioral Ecology and Sociobiology, 2001, 50: 483-492.
[9] 刘忠辉, 刘国道, 黄必志, 等. 云南猪屎豆属遗传多样性的ISSR分析[J]. 草业学报, 2009, 18(5): 184-191.
[10] Hamrick J L, Loveless M D. The genetic structure of tropical tree populations: Associations with reproductive biology[A]. In: Bock J U, Linhart Y B. Plant Evolutionary Ecology[M]. Boulder: Westview Press, 1989.
[11] Hamrick J L, Loveless M D. Factors influencing levels of genetic diversity in woody plant species[J]. New Forests, 1992, 6: 95-124.
[12] 刘锦, 贾睿芬, 贺晓, 等. 华北驼绒藜花粉萌发特性及不同授粉方式对其结实率的影响[J]. 种子, 2007, 26(9): 5-9.
[13] 王普昶, 易津, 赵丽丽. 华北驼绒藜种群数量动态与生殖特性的相关性[J]. 生态学杂志, 2010, 29(6): 1081-1086.
[14] 范彦, 曾兵, 张新全, 等. 中国野生鸭茅遗传多样性的ISSR研究[J]. 草业学报, 2006, 15(5): 103-108.
[15] Hogbin P M, Peakall R. Evaluation of the contribution of genetic research to the management of the endangered Zie ria prostrat[J]. Conservation Biology, 1999, 13: 514-522.
[16] 刘伟, 张新全, 李芳, 等. 西南区野生狗牙根遗传多样性的ISSR标记与地理来源分析[J]. 草业学报, 2007, 16(3): 55-61.
[17] Chuang M G. Allozyme diversity in the endangered shrub Abeliophyllum distichum(Oleaceae): a monotypic Korean genus[J]. International Journal of Plant Science, 1999, 160: 553-559.
[18] Bussell J D. The distribution of random amplified polymorphic DNA(RAPD) diversity amongst populations of Isotoma petraea (Lobeliaceae)[J]. Molecular Ecology, 1999, 8: 775-789.
[19] 张道远, Takamizo T. 剪股颖属植物遗传分化及系统关系的分子标记研究[J]. 草业学报, 2006, 15(3): 100-106.
[20] 陈小勇. 植物基因流及其在濒危植物保护中的作用[J]. 生物多样性, 1996, 4(2): 97-102.