Genetic diversity of Hemarthria compressa germplasms from southwestern China based on EST-SSR markers

Abstract

Abstract: Using EST-SSR with primers from cereal crops, the genetic diversity was assessed in 40 wild Hemarthria compressa clones from southwestern China and three varieties. After amplification with 23 pairs of EST-SSR primers of Poaceae, 323 fragments were detected, of which 261 were polymorphic, accounting for 80.4% of the total. The polymorphic information content was from 0.354 to 0.500. The genetic similarity coefficient of 43 accessions ranged from 0.690 to 0.913. Clustering analysis also revealed that the clustering among tested materials was related to their geographic origins, ecological types and morphological characteristics. AMOVA (analysis of molecular variance) of five geographical groups of H. compressa revealed that 95.32% of total variance occurred within these groups and 4.68% of total variance occurred between them. It is suggested that using EST-SSR primers from cereal crops, is feasible and effective for analyzing the genetic diversity of H.compressa.

CLC Number:

S812-05
Q943

CHEN Yong-xia, ZHANG Xin-quan, XIE Wen-gang, MA Xiao, LIU Ying. Genetic diversity of Hemarthria compressa germplasms from southwestern China based on EST-SSR markers[J]. Acta Prataculturae Sinica, 2011, 20(6): 245-253.

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