Acquisition and identification of cold-resistant somatic mutants of centipedegrass

Abstract

Abstract: Weak cold-resistance of centipedegrass is one of the major factors limiting its wide application. This study aimed to obtain cold-resistant somatic mutants from the seed of eminent Chinese native centipedegrass selection E-126 by induction and screening at low temperature. The callus induced by seeds of centipedegrass was cultivated at 0℃ for 26 d during the process of subculture and two pieces of survival callus tissues were obtained. Strain 1 and strain 2 were obtained after survival callus subculture proliferation, differentiation, rhizogenesis and transplantation. No significant differences were found in leaf color, lamellar hair, leaf length and leaf width between strains 1and 2 and the control. LT₅₀ of strains 1 and 2 were -6.646 and -6.546 ℃, respectively, which was significantly lower than that of control plants (-5.351 ℃) and there was no significant difference between the LT₅₀ of strains 1 and 2. The results of sequence related amplified polymorphism (SRAP) markers demonstrated that there were identical characteristic bands at 110, 230 and 240 bp of centipedegrass strains 1 and 2 obtained through low temperature induction. However, no characteristic bands at 110, 230 and 240 bp were found in control plants, indicating that the somatic mutants of centipedegrass were genetically stable compared with that of control plants at a molecular level. Strains 1 and 2 could be utilized as identical strains.

CLC Number:

YUAN Xue-jun, WANG Zhi-yong, ZHENG Yi-qi, LIU Jian-xiu, SHE Jian-ming. Acquisition and identification of cold-resistant somatic mutants of centipedegrass[J]. Acta Prataculturae Sinica, 2011, 20(6): 237-244.

References

[1] Bouton J H. Plant breeding characteristics relating to improvement of centipedegrass[J]. Soil and Crop Science Society of Florida Proceedings, 1983, 42: 53-58.
[2] Hanna W W. Centipede grass diversity and vulnerability[J]. Crop Science, 1995, 35: 332-334.
[3] Beard J B. Turfgrass Science and Culture[M]. Inc. Englewood Cliffs, N J: Prentice Hall, 1973: 151-183.
[4] Hanson A A, Juska F V, Burton G W. Species and varieties[J]. Turfgrass Science, 1969, 14: 370-409.
[5] Cardona C A, Duncan R R, Lindstrom O. Low temperature tolerance assessment in Paspalum[J]. Crop Science, 1997, 37: 1283-1291.
[6] Fry J D, Lang N S, Clifton G P, et al. Freezing tolerance and carbohydrate content of low-temperature-acclimated and nonacclimated centipedegrass[J]. Crop Science, 1993, 33: 1051-1055.
[7] Fry J D, Lang N S, Clifton R G P. Freezing resistance and carbohydrate composition of ‘Floratum’ St[J]. Augustinegrass. HortScience, 1991, 26: 1537-1539.
[8] Plilley H W, Watson C E, Krans J V, et al. Differentyal thermal analysis of St[J]. Augustynegrass HortScience, 1995, 30: 1388-1389.
[9] 宣继萍, 郭海林, 刘建秀, 等.中国假俭草种质资源抗寒性初步鉴定[J]. 草业学报, 2003, 12(6): 110-114.
[10] Johnson W J. Cold tolerance evaluation of several centipedegrass selections[J]. Agronomy Journal, 1977, 69: 100-103.
[11] Horn G C. Turfgrass variety comparisons[J]. Florida Turfgrass Management Conference Proceeding, 1967, 15: 91-92.
[12] Qi C H, Han L B. Progress on the application of transgenic technology on turfgrass breeding[J]. Grassland of China, 2002, 24: 47-54.
[13] Krans J V, Blanche F C B. Tissue culture of centipedegrass viewed from the egg[A]. In: Proceedings of the 5th International turfgrass Research Conference[C]. INRA Publication, 1985: 159-164.
[14] Ma S J, Zeng F H, Lan H T, et al. Tissue culture and plantlet regeneration of common centipede grass[J]. Plant Physiology Communication, 2004, 40: 62.
[15] 张芳, 王舟, 宗俊勤, 等. 农杆菌介导的假俭草遗传转化体系的建立[J]. 草业学报, 2011, 20(2): 184-192.
[16] Lazar M D, Chen T H, Gnsta H H, et al. Somaclonal variation for freezing tolerance in a population derived from Norstar winter[J]. Theoretical and Applied Genetics, l988, 75: 480-484.
[17] Edward J K, Jaded A Q, Kutty K K, et al. Regeneration of freezing-tolerant spring wheat (Triticum aestivum L.) plants from cryoselected callus[J]. Plant Physiology, 1990, 94: 1756-1762.
[18] Caliba G, Sutka J. Frost resistance somaclones derived from Triticum aestivum L.winter wheat calli[J]. Plant Breed, l989, 102: 101-104.
[19] 张婧源, 彭燕, 罗燕, 等. 不同产地白三叶种质遗传多样性的SRAP分析[J]. 草业学报, 2010, 19(5): 130-138.
[20] Budak H, Shearman R C, Parmaksiz I. Comparative analys of seeded and vegetative biotype bufalograsses based or phylogenetic relationship using ISSRs, SSRs, RAPDs, and SRAPs[J]. Theoretical and Applied Genetics, 2004, 109: 280-288.
[21] 朱根海, 朱培仁. 小麦抗冻性的季节变化及温度对脱锻炼的效应[J]. 南京农学院学报, 1984, 7(2): 9-16.
[22] Edwards K, Johnstone C, Thompson C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis[J]. Nucleic Acids Research, 1991, 19: 1349.
[23] Brar D S, Jain S M. Somaclonal: mechanism and applications in crop improvement[A]. In: Jain S M, Brar D S, Ahloowalia B S. Somaclonal Variation and Induced Mutations in Crop Improvement[M]. Nether-land: Kluwer Academic Publishers, 1998: 15-38.
[24] Smith R R, Quesenberry K H. Registration of NE WRC red clover germplasm[J]. Crop science, 1995, 35: 295.
[25] Croughan S S, Quisenberry S S, Eichhorn M M, et al. Registration of Brazos R3 bermudagrass germ-plasm[J]. Crop Science, 1994, 34: 542.
[26] Noh H Y, Choi J S, Ahn B J. Plant regeneration through somatic embryogenesis in zoysiagrasses (Zoysia spp.)[J]. Journal of the Korean Society for Horticultural Science, 1995, 36(4): 582-587.
[27] Griffin J D, Dibble M S. High-frequency plant regeneration from seed-derived callus cultures of Kentucky bluegrass(Poa pratensis L.)[J]. Plant Cell Reports, 1995, 14(11): 722-724.
[28] Ahn B J, Huang F H, King J W. Plant regeneration through somatic embryogenesis in common bermudagrass tissue culture[J]. Crop Science, 1985, 25(6): 1107-1109.
[29] Won S H, Lee B Hy. Plant Regeneration from seed-derived callus in perennial ryegrass[J]. Journal of Korean Grass Science, 2000, 20: 19-24.
[30] Cardona C A, Duncan R R. Callus induction and high efficiency plant regeneration via somatic am-bryogenesis in Paspalum[J]. Crop Science, 1997, 37(4): 1297-1303.
[31] 马生健, 曾富华, 蓝海婷, 等.假俭草的组织培养与植株再生[J]. 植物生理学通讯, 2004, 40(1): 62.
[32] Yoshikazu S, Tomonori N, Daisuke Y, et al. Induction of callus from seed and regeneration from the induced callus of warm season turfgrass[J]. Plant Cell Physiology Supplement, 2004, 45: 728-728.
[33] Takeuchi M, Tetuo N, Huruya P. Plant Tissue Culture Technology[M]. Asakura Publishing Co, Lid, 1984: 105-106.
[34] Jin Y Z, Du L, Wang J Y. Sifting a technology of bearing cold mutant from rice somatic cell[J]. Crop Variety Resources, 1999, 2: 27-29.
[35] Li G, Quiros C F. Sequence-related amplified polymorphisim (SRAP), a new marker systembased on a simple PCR reaction: its applicationto mapping and gene tagging in Brassica[J]. Theoretical and Applied Genetics, 2001, 103: 455-461.