Two strategies of cloning Medicago sativa phytochrome A and B genes

Abstract

Abstract: Short days are the main environmental factor leading to fall dormancy (FD) in alfalfa (Medicago sativa) which is considered to be a photoperiodic response with phytochromes as the main receptors. This study explored efficient methods for cloning phytochromes of M. sativa, including phytochrome A (PHYA) and phytochrome B (PHYB), which are the main phytochromes and have not been cloned in this species. Based on principles of comparative genomics and a bioinformatics approach, PHYA and PHYB genes were cloned by two different experimental strategies: using mRNA at the beginning and then the RACE technique, or by genomic DNA then genome walking. The sequences will be fundamental to studies of the relationship between FD in alfalfa and photoperiod. We also found thatM. truncatula could be used as a model plant in cloning unknown genes of M. sativa.

CLC Number:

LI Ping, YANG Ling-ling, CHEN Qi-xin, SHI Ying-hua, YAN Xue-bing, CHEN Zhan-kuan, WANG Cheng-zhang. Two strategies of cloning Medicago sativa phytochrome A and B genes[J]. Acta Prataculturae Sinica, 2011, 20(6): 85-92.

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