Cloning and analysis of the dehydrin (DHN) gene from Galega orientalis

Abstract

Abstract: Using rapid amplification of cDNA ends (RACE) method, a cDNA clone encoding dehydrin (GoDHN) was isolated from a cDNA SSH (suppression subtractive hybridization) library induced by salt stress from Galega orientalis. The full-length of cDNA sequence was 1 169 bp and included an 843 bp open reading frame. Molecular weight of this protein was 28.71 kDa. Results of real-time PCR indicated that the expression of quantity of GoDHN was higher in leaves and stems than in roots. Abiotic stress, such as NaCl and PEG, all induced the expression of GoDHN. Application of the exogenous hormone ABA also up-regulated mRNA accumulation. The expression of GoDHN increased with the induction time. These results indicated that the DHN gene may be involved in regulation of stress-resistance in G. orientalis. Construction of a pCAMBIA1302-DHN vector formed a basis for gene transformation.

CLC Number:

S816
Q943.2

Nikolay D, SUN Gui-zhi, GAO Hong-wen. Cloning and analysis of the dehydrin (DHN) gene from Galega orientalis[J]. Acta Prataculturae Sinica, 2012, 21(1): 176-183.

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