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Acta Prataculturae Sinica ›› 2012, Vol. 21 ›› Issue (1): 190-198.

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Genetic diversity and genetic relationships among three species of Medicago sativa complex in Xinjiang Province of China

LI Fei-fei1, CUI Da-fang2,3, YANG Hai-jun3, DENG Chao-hong1, LI Qing-yan3   

  1. 1.College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;
    2.College of Forestry, South China Agricultural University, Guangzhou 510642, China;
    3..Center of Experimental Teaching for Common Basic Courses, South China Agricultural University, Guangzhou 510642, China
  • Online:2012-02-20 Published:2012-02-20

Abstract: The genetic diversity and the genetic relationships among 10 populations of Medicago falcata, M. varia and M. sativa, belonging to the M. sativa complex were studied by using 15 pair of SSR primers and 10 ISSR primers. The results showed that population VG of M. varia had the highest percentage of polymorphism bands (PPB), Shannon’s information index (I) and Nei’s genetic diversity index (h) of 10 populations in SSR results; population FH of M. falcata had the 3 highest indices in ISSR results; population FH had private bands in SSR and ISSR results, which indicated VG of M. sativa and FH of M. falcata had the highest genetic diversity and thus need to be protected further. Three species could be distinguished clearly by UPGMA cluster analysis and combined their distribution in Xinjiang area, our study supported their classification at the species level.

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