Karyotype comparison of poppy (Papaver somniferum) isoform collected from different areas in China

Abstract

Abstract: Through raditional quashing method, we studied the chromosome number and karyotype of 2 poppy(Papaver somniferum) isoform with 10 samples, which were collected from five provinces in China. The results showed that two subtypes Isoform No.1 and No.2 were diploid with 2n=2x=22 chromosomes.The composition of relative length chromosomes for Isoform No.1 and No.2 was 2L+3M2+5M1+1S.The karyotype formula for Isoform No.1 was 18sm+4st, and the asymmetry index was 72.04% (“4A” type). The karyotype formula for Isoform No.2 was 16sm+6st,and the asymmetry index was 73.00% (“4B” type). The karyotype of different sample were highly asymmetrical, but slight differences were present among them.

CLC Number:

WEI Yu-jie, HE Qing-xiang, ZHANG Jin-wen, ZHANG Mei-xiu. Karyotype comparison of poppy (Papaver somniferum) isoform collected from different areas in China[J]. Acta Prataculturae Sinica, 2012, 21(2): 171-179.

References

[1] 仉文升, 李安良. 药物化学[M]. 北京: 高等教育出版社, 1999: 181-193.
[2] Bucardo J, Brouwer K C, Magis-Rodríguez C, et al. Historical trends in the production and consumption of illicit drugs in Mexico: implications for the prevention of blood borne infections[J]. Drug Alcohol Dependence, 2005, 79(3): 281-293.
[3] Keith R, Solomon, Arturo Anadón, et al. Coca and poppy eradication in Colombia: environmental and human health assessment of aerially applied glyphosate[J]. Reviews of Environmental Contamination Toxicology, 2007, 190: 43-125.
[4] Clark P A, Sillup G P, Capo J A. Afghanistan, poppies, and the global pain crisis[J]. Medical Science Monitor, 2010, 16(3): 49-57.
[5] Sayaka H, Shinichiro O, Eri A, et al. The effects of spacer sequences on silencing efficiency of plant RNAi vectors[J]. Plant Cells Reports, 2007, 26(5): 651-659.
[6] 梁倩倩, 张金文, 魏玉杰, 等. 野生罂粟COR、BBE基因片段融合及其RNAi载体构建[J]. 中国生物工程杂志, 2009, 29(11): 41-47.
[7] 吴征镒, 庄璇, 苏志云, 等. 中国植物志(第32卷)[M]. 北京: 科学出版社, 1999: 51-60.
[8] Hrishi N J. Cytological studies on Papaver somniferum L. and Papaver setigerum D. C. and their hybrid[J]. Genetica, 1960, 31(1): 1-30.
[9] Espinasse A, Dosba F . Cytological analysis of hybrids between Papaver somniferum L and Papaver bracteatum Lind phylogenetic relationships between the two species[J]. Agronomie, 1982, 2(3): 281-286.
[10] Patra N K, Chauhan S P. Morphophysiology and genetics of induced mutants expressed in the M₁ generation in opium poppies[J]. Journal of Heredity, 1990, 81(5): 347-350.
[11] Bra II, Bra C I, Cpraru G, et al. The possible ways of speciation in papaveraceae family[J]. Genetics and Molecular Biology, 2007, (8): 223-233.
[12] 卢龙斗, Bra II, 杜启彦. 罂粟属细胞在离体和活体培养中核型的比较研究[J]. 河南师范大学学报(自然科学版), 1995, 23(2): 61-68.
[13] 卢龙斗, 孙富丛, Bra II. 伪东方罂粟愈伤组织细胞核型研究[J]. 河南师范大学学报(自然科学版), 1996, 24(3): 66-70.
[14] 孙富丛, 路淑霞, 周延清, 等. 罂粟属三种植物的核型研究[J]. 河南职技师院学报, 1999, 27(2): 17-19.
[15] 张世良. 虞美人PMC减数分裂中的染色体及其核型分析[J]. 河南师范大学学报(自然科学版), 1992, 20(3): 129-132.
[16] 路金才. 药用植物学实验[M]. 北京: 中国医药科技出版社, 2006: 28-29.
[17] 李懋学, 张赞平. 作物染色体及其研究技术[M]. 北京: 中国农业科技出版社, 1996: 1-22.
[18] 吴姝菊, 李新玲, 唐凤兰. 大头稗(005)与鹅头稗(031)的生物学性状与细胞学研究[J]. 草业学报, 2011, 20(3): 198-204.
[19] 陈智勇, 胡忠红, 蒋建雄, 等. 早熟禾族3种基因型草坪草的染色体核型分析[J]. 草业学报, 2010, 19(6): 281-285.
[20] 李懋学, 陈瑞阳. 关于植物核型分析的标准化问题[J]. 武汉植物学研究, 1985, 3(4): 297-302.
[21] Levan A, Fredg A K, Sandberg A A. Nomenclature for centrometic position on chromosomes[J]. Hereditas, 1964, 52(2): 201-220.
[22] Stebbins G L. Chromosomal Evolution in Higher Plants[M]. London: Edward Arnold, 1971: 85-104.
[23] Arano H. Cytological studies in subfamily Carduoidae of Japan Ⅸ. The karyotype analysis and phylogenic consideration on Perty a and Ainsliaea(2)[J]. The Botanical Magazine, Tokyo, 1963, 76: 32-39.
[24] 张晓燕, 毛培春, 孟林, 等. 三份偃麦草种质的染色体核型分析[J]. 草业学报, 2011, 20(4): 194-201.
[25] ERZSBET, Mihalik E. Biology of poppy. Ⅱ. 1. Taxonomy[A]. In: Bernáth J. Poppy: The Genus Papaver[M]. USA: Harwood Academic Publishers, 1998: 8-10.
[26] 王琼, 苏智先, 宋会兴, 等. 植物染色体数目及其变异与生境关系初探[J]. 生态学杂志, 2001, 20(5): 8-11.
[27] 钟军, 王坤, 仇萍, 等. 鱼腥草不同地理群体遗传结构与变异的研究[J]. 草业学报, 2011, 20(2): 227-232.