Dynamic responses on anti-oxidative defense system and lipid peroxidation of Lespedeza davurica to drought stress and re-watering

Abstract

Abstract: The effects of different soil water levels [well-watered (CK), moderately stressed (DR₁) and severely stressed (DR₂)] and re-watering on antioxidant defense systems and membrane-lipid peroxidation of Lespedeza davurica was studied in a pot trial. The contents of malonaldehyde (MDA), ascorbic acid (AsA) and carotenoid (Car), as well as the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) in leaves of L. davurica under drought stress and re-watering were determined by sampling plants once every three days after the beginning of treatment, until 15 days of stress treatment. The results indicated that: 1) with increasing stress time, the activities of SOD, POD, CAT and APX in leaves were significantly higher in the water-stressed plants compared with well-watered plants. With an increase of drought stress intensity, SOD activity increased but activities of CAT and APX decreased. However, POD activity initially decreased but then increased after 6 days. In terms of anti-oxidants, the Car content in leaves were significantly higher in the water-stressed plants compared with well-watered plants, but the AsA content did not change significantly, although as the drought stress intensity increased, it initially decreased and then increased while the Car content was rising. Production of O₂^·- was increased by continuous drought stress treatment, and its accumulation resulted in lipid peroxidation, and in increased MDA content. In addition, as drought stress increased, MDA content increased in the first 6 days but there were no further significant changes in each treatment group although the O₂^·- content decreased. 2) After re-watering, the activities of APX and CAT, and the contents of Car and O₂^·- all increased, while the others decreased. 3) The antioxidant capacity of this plant was comprehensively evaluated by the subordinate function. L. davurica has higher drought resistance, and anti-oxidant capacity when moderately stressed than when severely stressed. Moderate stress may therefore be more suitable for the growth and development of this plant.

CLC Number:

Q945.78

DU Run-feng, HAO Wen-fang, WANG Long-fei. Dynamic responses on anti-oxidative defense system and lipid peroxidation of Lespedeza davurica to drought stress and re-watering[J]. Acta Prataculturae Sinica, 2012, 21(2): 51-61.

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