Abstract: Six parental cultivars of Lolium multiflorum and eighty F₂ progenies of nine cross combinations were used in this study. SRAP and SSR markers were used to analyze genetic relationship of 86 L. multiflorum accessions.15 and 16 primer pairs were selected by SRAP and SSR, respectively. 16.7 bands were amplified per primer pairs on average and percent of polymorphic bands was 68.88% in SRAP analysis. The average number of bands per primer pairs was 14.75 and percent of polymorphic bands was 58.05% by SSR markers. The conclusion of analyze by clustering tree showed that progenies from the same F₂ cross combination clustered together, except the combination Changjiang No.2×Jumbo, Changjiang No.2×Aubade;the genetic distance from F₂ progenies to male parent or female parent was so approximate. Meanwhile, the reliability of UPGMA dendrogram for L. multiflorum F₂ progenies and parental cultivars by SRAP markers was higher than SSR.