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Acta Prataculturae Sinica ›› 2012, Vol. 21 ›› Issue (4): 62-71.

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Effects of salt stress on seed germination and seedlings growth of salt-tolerant line and salt-sensitive line of maize

PENG Yun-ling, LI Wei-li,WANG Kun-ze, WANG Han-ning   

  1. Gansu Provincial Key Laboratory of Aridland Crop Science; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2012-02-17 Online:2012-04-25 Published:2012-08-20

Abstract: Salt stress affects plant growth and development and reduces productivity of crop. Maize (Zea mays) is moderate sensitive to salt. Screening and studying maize germplasm resistant to salt have certain directive function on improving salt-tolerant germplasm resources and breeding salt-tolerant variety, and have certain theoretical meaning of genetic mechanism of salt tolerance in maize. The study will focus on the comprehensive comparison of salt-tolerant properties change of salt-tolerant lines 8723, 81162 and salt-sensitive line P138 at germination and seedling stages under salt stress, in order to probe into salt-tolerant mechanism in maize, to provide the materials for breeding resistant-salt variety and discovering genes related to salt tolerance and to lay the foundation for salt-tolerant genetic mechanism in maize.Seed germination and seedling growth of salt-tolerant lines 8723, 81162 and salt-sensitive line P138 of maize under different concentrations of NaCl stress were determined in this experiment. The experimental results indicated that, with the increase of salt concentration, there were higher germination rate, germination potential, germination index, activity index, plumule length, and lower salt injury index, of salt-tolerant line with a small change at seed germination stage, compared with salt-sensitive line. A study of salt-tolerant lines and salt-sensitive line under different concentrations of NaCl stress in seedling stage showed that the height of seedlings, root length, fresh weight of the three inbred lines all decreased with increasing concentrations of NaCl. The decrease rate of these indices for the salt-tolerant lines was all less than those for the salt-sensitive inbred line. The results of physiological changes under different concentrations of salt stress indicated that there were higher relative water content and lower relative conductivity of organization of external seepage of salt-tolerant lines with a small change, compared with salt-sensitive line. To analyze all experimental results, salt-tolerant line 8723 had stronger tolerant than 81162.

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