Construction and analyses of two suppression subtractive hybridization libraries of Medicago falcata and Medicago truncatula under drought stress

Abstract

Abstract: Drought is one of the commonest abiotic stresses for plant growth and development. Understanding the mechanisms underlying the tolerance of plants to drought stress is of critical importance for the improvement of plant drought tolerance. Suppression subtractive hybridization (SSH) is a useful tool to identify genes that are differentially expressed. In this study, two cDNA libraries from the leguminous plants Medicago falcata and M. truncatula were constructed and sequenced. The sequences of the two cDNA libraries were classified by GO and KEGG, and the differential expression was analyzed. The number of genes associated with drought resistance was greater in M. falcata than in M. truncatula. The expression levels of the two genes were positively correlated with the tolerance of the two species to drought stress and were higher in M. falcata than in M. truncatula. These results may provide a molecular explanation for some of the greater tolerance to drought stress of M. falcata than M. truncatula.

CLC Number:

WANG Tian-zuo, ZHAO Min-gui, ZHANG Wen-hao. Construction and analyses of two suppression subtractive hybridization libraries of Medicago falcata and Medicago truncatula under drought stress[J]. Acta Prataculturae Sinica, 2012, 21(6): 175-181.

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