Physiological responses to PEG stress of Reaumuria soongorica seedlings from different geographical origins

Abstract

Abstract: The effects of different concentrations of PEG-6000 stress on relative permeatbility of plasma membrane, protective enzyme activity, and osmotic gulatory molecules in leaves of Reaumuria soongorica seedlings from Lanzhou, Wuwei, Zhangye and Jiuquan geographic origins in Gansu province were studied. Contents of proline (Pro) and soluble sugars (SS) in leaves initially increased but then decreased as the stress concentrations increased. The accumulation in plants from Wuwei was more than that in those from other origins. Contents of malondiadehyde (MDA) and relative permeability of plasma (RPP) increased as stress concentrations increased but the increased range in Wuwei origin plants was less than that of others, which indicated a stronger drought resistances than plants from the other origins. Protective enzyme activities such as superoxide dismutase (SOD), peroxide enzyme (POD) and catalase (CAT) increased initially but then decreased. POD and CAT activities of Wuwei origin plants were higher than those from other origins. There was a threshold of protective enzyme activity, and it was reduced when protective enzyme activity was out of the threshold level. Correlation analysis showed that there were significant positive correlations of relative permeability of plasma with MDA (P＜0.05), highly significant positive correlations with Pro and SS (P＜0.01), and significant negative correlations of relative permeability of plasma with SOD, CAT, and POD activity (P＜0.05). Drought resistance of R. soongorica from four geographic origins was in the following order Wuwei>Jiuquan>Zhangye>Lanzhou.

CLC Number:

Q945.78

CHONG Pei-fang, SU Shi-ping, LI Yi, SUN Zhao-cheng. Physiological responses to PEG stress of Reaumuria soongorica seedlings from different geographical origins[J]. Acta Prataculturae Sinica, 2013, 22(1): 183-192.

References

[1] 刘家琼, 邱明新, 蒲锦春, 等. 我国典型超旱生植物——红砂[J]. 植物学报, 1982, 24(5): 485-488.
[2] 马茂华, 孔令韶. 新疆呼图壁绿洲外缘的琵琶柴生物生态学特性研究[J]. 植物生态学报, 1998, 22(3): 237-244.
[3] 黄培祐, 聂湘萍. 准噶尔盆地中部琵琶柴群落的生境研究[J]. 新疆大学学报, 1988, 5(3): 66-71.
[4] 曾彦军, 王彦荣, 保平, 等. 几种生态因子对红砂和霸王种子萌发与幼苗生长的影响[J]. 草业学报, 2005, 14(5): 24-31.
[5] 王孝安. 安西荒漠植物群落和优势种的分布与环境的关系[J]. 植物学报, 1998, 40(11): 1047-1052.
[6] 马剑英, 周邦才, 夏敦胜, 等. 荒漠植物红砂叶绿素和脯氨酸累积与环境因子的相关分析[J]. 西北植物学报, 2007, 27(4): 769-775.
[7] 贾荣亮, 周海燕, 谭会娟, 等. 超旱生植物红砂与珍珠光合生理生态日变化特征初探[J]. 中国沙漠, 2006, 26(4): 631-636.
[8] 冯亮亮, 唐红, 李毅, 等. 甘肃红砂不同种群遗传多样性的ISSR分析[J]. 草业学报, 2011, 20(1): 125-130.
[9] 刘玉冰, 张腾国, 李新荣, 等. 红砂忍耐极度干旱的保护机制: 叶片脱落和茎中蔗糖累积[J]. 中国科学C辑生命科学, 2006, 36(4): 328-333.
[10] 王瑾, 刘桂茹, 杨学举. PEG胁迫下小麦再生植株根系特性与抗旱性的关系[J]. 麦类作物学报, 2006, 26(3): 117-119.
[11] 许桂芳. PEG胁迫对2种过路黄抗性生理生化指标的影响[J]. 草业学报, 2008, 17(1): 66-70.
[12] 高暝, 李毅, 种培芳, 等. 渗透胁迫下不同地理种源白刺的生理响应[J]. 草业学报, 2011, 20(3): 99-107.
[13] 王丽华, 姜春玲, 冯玉龙. 不同地理种源长白落叶松生理生态特性的研究[J]. 植物研究, 1999, 19(2): 165-171.
[14] 郝再彬, 苍晶, 徐仲. 植物生理实验[M]. 哈尔滨: 哈尔滨大学出版社, 2004: 101-108.
[15] 王学奎, 章文华, 郝再彬. 植物生理生化原理与技术[M]. 北京: 高等教育出版社, 2006: 20.
[16] 朱建军, 王洪春. 生物膜渗透特性的理论分析[J]. 植物生理学通讯, 1986, (1): 6-10.
[17] 李霞, 阎秀峰, 于涛. 水分胁迫对黄檗幼苗保护酶活性及脂质过氧化作用的影响[J]. 应用生态学报, 2005, 16(12): 2353-2356.
[18] 聂磊, 刘鸿先, 彭少麟. 水分胁迫对长期UV-B辐射下柚树苗生理特性的影响[J]. 植物资源与环境学报, 2001, 10(3): 19-24.
[19] 韩蕊莲, 李丽霞, 梁宗锁, 等. 干旱胁迫下沙棘膜脂过氧化保护体系研究[J]. 西北林学院学报, 2002, 17(4): 1-5.
[20] Erra J R, Sinelair T R. Osmolyte accumulation: can it really help increase crop yield under drought conditions[J]. Plant Cell and Environment, 2002, 25: 333-341.
[21] 曹仪植. 水分胁迫下小麦体内游离脯氨酸积累及其ABA 在其中的作用[J]. 植物生理学报, 1985, 11(1): 9-16.
[22] 谢贤健, 兰代萍, 白景文. 三种野生岩生草本植物的抗旱性综合评价[J]. 草业学报, 2009, 18(4): 75-80.
[23] 韩瑞宏, 卢欣石, 高桂娟, 等. 紫花苜蓿抗旱性主成分及隶属函数分析[J]. 草地学报, 2006, 14(2): 142-146.
[24] 种培芳, 苏世平, 李毅. 4个地理种群红砂的抗旱性综合评价[J]. 草业学报, 2011, 20(5): 26-33.