Cloning of a S-adenosyl methionine synthetase gene from Cleistogenes songorica and its expression under drought stress

Abstract

Abstract: To identify more genes for the improvement of drought-tolerance in plants, Cleistogenes songorica was used to investigate differential gene expression under drought stress. A full-length cDNA of the S-adenosyl methionine synthetase (SAMS) gene was isolated from C. songorica. It was named CsSAMS1 and was 1 399 bp in length and encoded 397 amino acids with the three typical domains of SAMS. The CsSAMS1 protein was a hydrophilic protein with no transmembrane domain but with an α-helix spatial structure constituting a random coil. Multiple alignment analysis based on amino acids of SAMS genes from kindred plants indicated that SAMS was very conserved between different species with 92%-99% similarity in amino acid level. CsSAMS1 had the highest similarity with that of Oryza sativa (99%). The expression patterns of CsSAMS1 under drought were investigated by semi-quantitative and real-time quantitative RT-PCR analysis. The results showed that drought stress induced a large amount of gene expression in roots, but there was no significant change in leaves. The CsSAMS1 gene was induced by drought stress, and it is a basis for further study on genetic improvement of grass drought resistance.

CLC Number:

KONG Ling-fang, ZHANG Ji-yu, LIU Zhi-peng, WANG Yan-rong. Cloning of a S-adenosyl methionine synthetase gene from Cleistogenes songorica and its expression under drought stress[J]. Acta Prataculturae Sinica, 2013, 22(1): 268-275.

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