In vitro mutation induction by NaN3 in ryegrass variety “Blue Heaven” and RAPD analysis for its variation

Abstract

Abstract: The ryegrass (Lolium multiflorum) variety “Blue Heaven” were treated by sodium azide (NaN₃). After selecting from M₂ generation and identification of M₃ generation, we obtained 60 mutants with the most significant variation traits. Two panicle mutants were chosen to identify by molecular markers. 100 RAPD (randomly amplified polymorphic DNA) random primers were used to analyze check (CK) and 2 panicle mutants. The results showed that 60 plants with stable variation traits were obtained from M₂ mutant lines. 21 panicle mutants were obtained and it was the most among these mutant types. 2 panicle mutants were compared with CK. Only one top spike on CK, 2 panicle mutants have more top spikelets. And mutants No.2 and CK have not secondary branch per panicle. Therefore, mutant No.1 has 5 secondary panicle braches. 45 RAPD primers can be amplified among the three materials. In addition, the three materials can be differentiated by 3 RAPD primers. So there were difference between CK and the 2 panicle mutants on molecular level.

CLC Number:

S543⁺.6

LI Jie-qin, WANG Li-hua, ZHAN Qiu-wen, DONG Hai-feng, LIU Jing-liang, LI Wan-xiang. In vitro mutation induction by NaN₃ in ryegrass variety “Blue Heaven” and RAPD analysis for its variation[J]. Acta Prataculturae Sinica, 2013, 22(1): 276-281.

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In vitro mutation induction by NaN₃ in ryegrass variety “Blue Heaven” and RAPD analysis for its variation

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