Isolation and characterization of PGPR from the rhizosphere of the Avena sativa in saline-alkali soil

Abstract

Abstract: Two strains of plant growth-promoting rhizobacteria (PGPR) containing ACC deaminase, A-2 and A-4 were isolated from saline-alkali rhizosphere soil of Avena sativa, based upon their ability to utilize 1-aminocyclopropane-1-carboxylate (ACC) as a sole nitrogen source. Using morphology, physiological biochemical characteristics and 16S rDNA analysis, strain A-2 was identified as Enterobacter sp. and A-4 was confirmed as Pseudomonas sp.. The ACC deaminase activity of A-2 and A-4 were (1.89±0.12) μmol α-KA/(mg Pr·h) and (2.63±0.12) μmol α-KA/(mg Pr·h), respectively. When the concentration of L-Trp increased, the IAA synthesis of A-2 increased, while that of A-4 did not change significantly. The ability of A-2 to synthesize siderophore was greater than that of A-4. The height of oat plants treated with A-2 and A-4 were from 12.24 cm up to 13.98 and 14.07 cm, the plant fresh weights were from 34.285 g up to 35.012 and 35.102 g, respectively. The length of roots were from 14.13 cm up to 16.23 and 17.60 cm, and root fresh weights were from 7.790 g up to 8.065 and 8.187 g, respectively. These results suggest that strains A-2 and A-4 contain ACC deaminase that can increase resistance in oat to saline-alkali stress.

CLC Number:

Q945.3

LIU Jia-li, FANG Fang, SHI Xu-han, CHEN Hong-yan, YAO Lin, GUO Chang-hong. Isolation and characterization of PGPR from the rhizosphere of the Avena sativa in saline-alkali soil[J]. Acta Prataculturae Sinica, 2013, 22(2): 132-139.

References

[1] Penrose D M, Glick B R. Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria[J]. Physiologia Plantarum, 2003, 118(1): 10-15.
[2] 胡江春, 薛德林, 马成新, 等. 植物根际促生菌(PGPR)的研究与应用前景[J]. 应用生态学报, 2004, 15(10): 1963-1966.
[3] 孟宪法, 隆小华, 康健, 等. 菊芋内生固氮菌分离、鉴定及特性研究[J]. 草业学报, 2011, 20(6): 157-163.
[4] 郭丽琢, 张虎天, 何亚慧, 等. 根瘤菌接种对豌豆/玉米间作系统作物生长及氮素营养的影响[J], 草业学报, 2012, 21(1): 43-49.
[5] 冯欣, 刁治民, 曹玲珍, 等. PGPR作为微生物肥料的研究进展[J]. 安徽农学通报, 2005, 11(6): 85-87.
[6] Belimov A A, Hontzeas N, Safronova V I, et al. Cadmium-tolerant plant growth-promoting bacteria associated with the roots of Indian mustard[J]. Soil Biology and Biochemistry, 2005, 37: 241-250.
[7] 姚军朋, 姚拓, 王小利. ACC脱氨酶的应用研究进展与评述[J]. 生物技术, 2010, 20(2): 87-91.
[8] 高文俊, 徐静, 谢开云, 等. Na₂CO₃和NaHCO₃胁迫下冰草的生长及生理响应[J]. 草业学报, 2011, 20(4): 299-304.
[9] 纪荣花, 于磊, 鲁为华, 等. 盐碱胁迫对芨芨草种子萌发的影响[J]. 草业科学, 2011, 28(2): 245-250.
[10] 赵玲, 柴兆祥, 李金花, 等. 四株胡萝卜软腐欧文氏杆菌胡萝卜亚种新菌株的分离鉴定[J]. 草业学报, 2011, 20(4): 244-251.
[11] 李振东, 陈秀蓉, 李鹏, 等. 珠芽蓼内生菌Z5产IAA和抑菌能力测定及其鉴定[J]. 草业学报, 2010, 19(2): 61-68.
[12] Schwyn B, Neilands J B. Universal chemical assay for the detection and determination of siderophores[J]. Analytical Biochemistry, 1987, 160: 47-56.
[13] 王平, 董飚, 李阜棣, 等. 小麦根圈细菌铁载体的检测[J]. 微生物学通报, 1994, 21(6): 323-326.
[14] Li J, Ovabin D H, Charles T C, et al. An ACC deaminase minus mutant of Enterobacter cloacae UW4 no longer promotes root elongation[J]. Current Microbiology, 2000, 41: 101-105.
[15] Penrose D M, Glick B R. Levels of ACC and related compounds in exudates and extracts of canoda seeds treated with ACC deaminase-containing plant growth-promoting bacteria[J]. Canadian Journal of Microbiology, 2001, 47: 368-372.
[16] Ma W, Penrose D M, Glick B R. Strategies used by rhizobia to lower plant ethylene levels and increase nodulation[J]. Canadian Journal of Microbiology, 2002, 48(11): 947-954.
[17] Belimov A A, Dodd I C, Hontzeas N, et al. Rhizosphere bacteria containing 1-aminocyclopropane-1-carboxylate deaminase increase yield of plants grown in drying soil via both local and systemic hormone signaling[J]. New Phytologist, 2009, 181(2): 413-423.
[18] Farwell A J, Vesely S, Nero V, et al. Tolerance of transgenic canola plants (Brassica napus) amended with plant growth-promoting bacteria to flooding stress at a metal-contaminated field site[J]. Environmental Pollution, 2007, 147(3): 540-545.
[19] Gamalero E, Berta G, Massa N, et al. Interactions between Pseudomonas putida UW4 and Gigaspora rosea BEG9 and their consequences for the growth of cucumber under salt-stress conditions[J]. Journal of Applied Microbiology, 2010, 108(1): 236-245.
[20] Sheng X F, He L Y, Zhou L, et al. Characterization of Microvacterium sp. F10a and its role in polycyclic aromatic hydrocarbon removal in low-temperature soil[J]. Canadian Journal of Microbiology, 2009, 55(5): 529-535.
[21] 孙乐妮, 何琳燕, 张艳峰, 等. 海州香薷(Elsholtzia splendens)根际铜抗性细菌的筛选及生物多样性[J]. 微生物学报, 2009, 49(10): 1360-1366.
[22] 赵骥民, 辛树权, 何正飚, 等. 碱胁迫下PGPR菌株对水稻萌发及水稻幼苗生长的促生作用[J]. 安徽农业科学, 2007, 35(34): 11060-11062.
[23] 辛树权, 王贵, 高扬, 等. 植物促生菌CS2,CC9和草炭土添加量对盐碱土育稻秧的作用效果[J]. 广东农业科学, 2011, 38(7): 82-85.
[24] Mayak S, Tirosh T, Glick B R. Plant growth-promoting bacteria confer resistance in tomato plants to salt stress[J]. European Journal of Medicinal Chemistry, 2004, 42(6): 565-572.
[25] Saima M, Shazia A, Shahida H. Effect of bacterial monoculture inoculations on the early growth of Triticum aestivum var. Inqlab-91 under NaCl stress[J]. Pakistan Journal of Biological Sciences, 2002, 5(6): 643-647.
[26] Jalili F, Khavazi K, Pazira E, et al. Isolation and characterization of ACC deaminase-producing fluorescent pseudomonads to alleviate salinity stress on canola (Brassicanapus L.) growth[J]. Journal of Plant Physiology, 2009, 166(6): 667-674.
[27] Glick B R, Liu C, Ghosh S, et al. Early development of canola seedlings in the presence of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2[J]. Soil Biology and Biochemistry, 1997, 29(8): 1233-1239.
[28] Kloepper J W, John L, Teintze M. Enhanced plant growth by siderophore produced by plant growth-promoting rhizobacteria[J]. Nature, 1980, 286: 885-886.
[29] Siebner F H, Hadar Y, Chen Y. Siderophores sorbed on Ca-montmorillonite as an iron source for plants[J]. Plant Soil, 2003, 251: 115-124.