Cloning and expression analysis of the promoter of Caragana korshinskii gene

Abstract

Abstract: A genomic walking technique was used to clone the promoter sequence of the CkNCED1 gene from Caragana korshinskii. The plant CARE cis-element analysis revealed that, besides the basic elements, the promoter sequence included two ABA responsive elements (ABRE) and many other stress-response elements. The PGWB533 expression vector containing this promoter sequence was constructed with the GUS reporter gene. Histochemical staining analysis showed that the GUS reporter gene was expressed in the tissues of leaves, root, stem, flower and silique, and was especially higher in leaves. These results identified the localization and expression of the CkNCED1 gene in vivo, which suggested that CkNCED1 gene plays an important role in biosynthesis regulation of ABA. The study lay a foundation for further analysis of the gene function.

CLC Number:

REN Ai-qin, YI Jin, GAO Hong-wen, LI Jun, WANG Xue-min. Cloning and expression analysis of the promoter of Caragana korshinskii gene[J]. Acta Prataculturae Sinica, 2013, 22(2): 165-170.

References

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