Effects of bio-fertilizers with different PGPR strain combinations on yield and quality of alfalfa

doi:10.11686/cyxb20130512

Abstract

Abstract: In recent years, bio-fertilizers have emerged as an important component of the integrated nutrient supply system and hold great promise to improve crop yields because increasing use of chemical fertilizers and high productive systems are creating environmental problems. The effects of different combinations of PGPR strains on yield and quality of alfalfa (Medicago sativa) were studied in randomized block field experiments using bio-fertilizers. These were produced using different combinations of three phosphate solubilizing bacteria (Bacillus sp., Pseudomonas sp. and Azotobacter sp.) and one rhizobium (Sinorhizobium meliloti) strain isolated from the rhizosphere of alfalfa and wheat. Hay yield and quality of alfalfa decreased when treated with mono-strain bio-fertilizer (containing 50% phosphatic fertilizer) compared with the control (100% phosphatic fertilizer) although there was no significant difference. Hay yield, content of crude protein (CP), calcium, phosphate and ether extract (EE) increased 10.6%, 16.4%, 14.1%, 11.9% and 4.2% respectively, while acid detergent fiber (ADF) and neutral detergent fiber (NDF) were reduced by 10.9% and 7.7%. The benefit of mono strain bio-fertilizer was inferior to that of mixed strain bio-fertilizer. Strains S7 and Jm170+Jm92+Lx191+S7 with 50% phosphatic fertilizer are recommended as the best treatments to substitute for 100% phosphatic fertilize in alfalfa production.

CLC Number:

HAN Hua-wen, SUN Li-na, YAO Tuo, RONG Liang-yan, LIU Qing-hai, LU Hu, MA Hui-ling. Effects of bio-fertilizers with different PGPR strain combinations on yield and quality of alfalfa[J]. Acta Prataculturae Sinica, 2013, 22(5): 104-112.

References

葛均青, 于贤昌, 王竹红. 微生物肥料效应及其应用展望. 中国生态农业学报, 2003,11(3): 87-88.
刘健, 李俊, 葛诚. 微生物肥料作用机理的研究新进展. 微生物学杂志, 2001, 21(1): 33-36.
曹宏,章会玲, 盖琼辉, 等. 22个紫花苜蓿品种的引种试验和生产性能综合评价. 草业学报, 2011, 20(6): 219-229.
任立飞, 张文浩, 李衍素. 低磷胁迫对黄花苜蓿生理特性的影响. 草业学报, 2012, 21(3): 242-249.
谭周进, 肖罗, 谢丙炎, 等.假单胞菌的微生态调节作用. 核农学报, 2004, 18(1): 72-76.
韩文星, 姚拓, 席琳乔, 等. PGPR菌肥制作及其对燕麦生长和品质影响的研究. 草业学报, 2008, 17(2): 75-84.
韩光, 张磊, 邱勤, 等. 复合型PGPR和苜蓿对新垦地土壤培肥效果研究. 土壤学报, 2011, 48(2): 405-411.
李玉娥, 姚拓, 荣良燕. 溶磷菌溶磷和分泌IAA特性及对苜蓿生长的影响. 草地学报, 2010, 18(1): 84-88.
刘慧霞, 申晓蓉, 郭正刚. 硅对紫花苜蓿种子萌发及幼苗生长发育的影响. 草业学报, 2011, 20(1): 155-160.
Esitken A, Yildiz H E, Ercisli S, et al. Effects of plant growth promoting bacteria (PGPB) on yield, growth and nutrient contents of organically grown strawberry. Scientia Horticulturae, 2010, 124:62-66.
张堃, 姚拓, 张德罡, 等. 高寒地区联合固氮菌肥对青稞的促生效应研究. 植物营养与肥料学报, 2010, 16 (3): 708-713.
Farzana Y, Radziah O, Kamaruzaman S, et al. Effect of PGPR Inoculation on growth and yield of sweetpotato. Journal of Biological Sciences, 2007, 7(2): 421-424.
Ashrafuzzaman M, Farid A H, Razi Ismail M, et al. Efficiency of plant growth-promoting rhizobacteria(PGPR) for the enhancement of rice growth.African Journal of Biotechnology, 2009, 8 (7): 1247-1252.
Mohammad Y, Mohammad A B, Hemmatollah P, et al. Effect of phosphate solubilization microorganisms (PSM) and plant growth promoting rhizobacteria (PGPR) on yield and yield components of corn (Zea mays L.).World Academy of Science, Engineering and Technology, 2009, 49: 90-91.
甘肃农业大学. 草原生态化学实验指导书. 北京: 农业出版社, 1987.
姚拓. 高寒地区燕麦根际联合固氮菌研究Ⅱ.固氮菌的溶磷性和分泌植物生长素特性测定. 草业学报, 2004,13(3): 85-90.
姚拓, 蒲小鹏, 张德罡, 等.高寒地区燕麦根际联合固氮菌研究Ⅰ.固氮菌对燕麦生长的影响及其固氮量测定. 草业学报, 2004, 13(5): 101-105.
席琳乔, 姚拓, 张德罡. 固氮菌对燕麦不同生育期促生作用的研究. 草业学报, 2007,16(3): 38-42.
占新华, 蒋延惠, 徐阳春, 等.微生物制剂促进植物生长机理的研究进展. 植物营养与肥料学报,1999,5(2): 97-105.
张余莽, 周海军, 张景野, 等. 生物有机肥的研究进展. 吉林农业科学,2010, 35(3): 37-40.
Chen M M, Chen B D, Marschner P. Plant growth and soil microbial community structure of legumes and grasses grown in monoculture or mixture. Journal of Environmental Sciences, 2008, 20: 1231-1237.
同小娟, 李俊, 李维炯. 长期施用有效微生物肥对冬小麦-夏玉米生长和产量的影响. 华北农业学报, 2007, 27(6): 165-170.
刘旭, 沼孝侯, 毛欣, 等.EM生物有机肥对甜玉米生长发育及土壤特性的影响. 江苏农业科学, 2010, (1): 301-304.
李玉华, 许前欣, 李明悦, 等.微生物菌肥对库尔勒香梨产量、品质的影响. 天津农业科学, 2006, 12(2): 48-50.
希斯M E.牧草一草地农业科学. 北京: 农业出版社,1989
王富民, 张彦, 吴皓琼, 等. 解磷固氮菌剂的研制及其对小麦的增产效应. 生物技术, 1994, 4(4): 15-18.
饶正华, 林启美, 孙焱鑫, 等. 解钾菌与解磷菌及固氮菌的相互作用. 生态学杂志, 2002, 21(2): 71-73.
冯瑞章, 姚拓, 周万海, 等. 溶磷菌和固氮菌溶解磷矿粉时的互作效应. 生态学报, 2006, 26(8): 2764-2769.
Hortencia G M, Victor O. Alteration of tomato fruit quality by root inoculation with plant growth-promoting rhizobacteria (PGPR): Bacillus subtilis BEB-13bs. Scientia Horticulturae, 2007, 113(1): 103-106.
李保会, 李青云, 李建军, 等. 复合微生物菌肥对连作草莓产量和品质的影响. 河北农业科学, 2007, 11(1): 15-19.
易艳梅, 黄为一. 溶磷细菌Enterobacteria sp. EnHy-401对盐渍土壤中小麦生长的影响. 土壤通报, 2011, 42(6): 1391-1395.
Yanni Y G, Rizk R Y, El-Fattah F K, et al. The beneficial plant growth-promoting association of Rhizobium leguminosarum bv. trifolii with rice roots. Australian Journal of Plant Physiology, 2001, 28: 845-870.
栗丽, 洪坚平, 谢英荷, 等. 生物菌肥对采煤塌陷复垦土壤生物活性及盆栽油菜产量和品质的影响. 中国生态农业学报, 2010, 18(5): 939-944.
张堃, 姚拓, 张德罡, 等.不同剂型联合固氮菌肥对青稞促生效应和固氮能力研究. 草地学报, 2010, 18(3): 426-430.
Yanni Y G, Rizk R Y, Corich V et al. Natural endophytic association between Rhizobium leguminosarum bv. trifolii and rice roots and assessment of its potential to promote rice growth. Plant and Soil, 1997, 194: 99-114.
Govindarajan M, Balandreau J, Muthukumarasamy R, et al. Improved yield of micropropagated sugarcane following inoculation by endophytic Burkholderia vietnamiensis. Plant and Soil, 2006, 280: 239-252.

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