Abstract: Seashore paspalum (Paspalum vaginatum) is a salt-tolerant plant that does not require good-quality water for irrigation. Therefore, it has the potential to greatly reduce fresh water consumption and waste. To date, however, no new seashore paspalum cultivars have been bred in China. Mutation is an important method for breeding new varieties of plants, including turfgrass. The objective of this study was to breed new strains of seashore paspalum with the ⁶⁰Co-γ radiation method. Sprigs of “Sea Isle 2000”, “Platinum”, “Supreme”, and “Salam” were radiated at five different radiation intensities, and then favorable mutants were screened by comparing leaf width, leaf length, plant height, stolon length, stolon diameter, and stolon density with those of unirradiated controls. Nine mutants were acquired by ⁶⁰Co-γ irradiation of sprigs of seashore paspalum. Compared with the control plants, the mutants showed greater plant height, leaf length, and leaf width. The turf qualities of the mutants “SI-50-1”, “PL-40-2” and “SLM-45-1” were better than those of the control. In conclusion, we have identified the appropriate radiation intensity of ⁶⁰Co-γ for mutation of seashore paspalum, and we have generated several new mutants of this important plant.