Effects of germplasm and changing temperature on seed germination of Leymus chinensis

doi:10.11686/cyxb2017327

Abstract

Abstract: To clarify the reasons for low seed germination rate, germination of 43 different germplasm lines of sheep grass was studied at constant temperatures of 16, 20, 22, 28 and 37 ℃, and under a variable temperature regime of 28 ℃ (12 h) and 16 ℃ (12 h). It was found that seed germination percentage of sheep grass differed between germplasm lines, with values ranging from 10% to 95%. Seed germination was low under constant temperature conditions, while the alternating 28 ℃/16 ℃ treatment increased the seed germination rate by 50%-90% (P<0.01). Furthermore, the longer the daily high temperature exposure, the higher germination rate was. The seed germination rate was increased very significantly (P<0.01) on 1 day or 2 days of variable temperature treatment, compared with the constant temperature control. However, on the first day of germination, variable temperature less than 2 hours cannot significantly promotes germination, and germination rate improves significantly in 8-12 hours treatments, which considers as the “window period”.

Key words: sheep grass, germplasm, seed germination, variable temperature treatment

YANG Wei-guang, LIU Pan-pan, YUAN Guang-xiao, MAO Pei-sheng, QI Dong-mei, DONG Xiao-bing, LIU Hui, LI Xiao-xia, LIU Gong-she. Effects of germplasm and changing temperature on seed germination of Leymus chinensis[J]. Acta Prataculturae Sinica, 2018, 27(7): 103-111.

References

[1] Zhao C X, Yang G F, Zhang G T, et al.Study on germination rate of sheepgrass seeds. Grassland of China, 1986, (5): 54-56, 80.
赵传孝, 杨根凤, 张国瞳, 等. 羊草种子发芽率的研究. 中国草原, 1986, (5): 54-56, 80.
[2] Yi J, Li Q F, Tian R H.Seed dormancy and hormone control of germination in Leymus Hochst. Acta Agrestia Sinca, 1997, (2): 93-100.
易津, 李青丰, 田瑞华. 赖草属牧草种子休眠与植物激素调控. 草地学报, 1997, (2): 93-100.
[3] Qi D M, Zhang W D, Liu G S.Leymus chinensis seed viability testing technology. Acta Prataculturae Sinica, 2004, (2): 89-93.
齐冬梅, 张卫东, 刘公社. 羊草种子生活力测定技术研究. 草业学报, 2004, (2): 89-93.
[4] Ma H Y.Mechanical lemma stress significantly inhibited the seed germination of proceedings of the symposium on plant stress physiology and molecular biology//Summary of papers of the national symposium on plant stress physiology and molecular biology in 2005. Zhanjiang: Committee of Environmental Physiology and Nutrition Physiology of the Chinese Society of Plant Physiology, 2005: 1.
马红媛. 外稃的机械物理胁迫显著抑制羊草种子萌发//2005年全国植物逆境生理与分子生物学研讨会论文摘要汇编. 湛江: 中国植物生理学会环境生理与营养生理专业委员会, 2005: 1.
[5] Ma H Y, Liang Z W, Chen Y.Research progress on improving germination rate of Leymus chinensis. Grassland of China, 2005, (4): 64-68.
马红媛, 梁正伟, 陈渊. 提高羊草种子发芽率方法研究进展. 中国草地, 2005, (4): 64-68.
[6] Lin J X, Shao S, Sui D, et al.Comparisons of several methods of improving seed germination percentage of Leymus chinensis. Chinese Journal Grassland, 2014, (3): 47-51.
蔺吉祥, 邵帅, 隋丹, 等. 几种提高羊草种子发芽率方法的比较. 中国草地学报, 2014, (3): 47-51.
[7] Ma H Y, Liang Z W.Effects of storage conditions and sowing methods on seed germination of Leymus chinensis. Chinese Journal of Applied Ecology, 2007, (5): 999-1004.
马红媛, 梁正伟. 不同贮藏条件和发芽方法对羊草种子萌发的影响. 应用生态学报, 2007, (5): 999-1004.
[8] He X Q, Hu X W, Wang Y R.Study on seed dormancy mechanism and breaking technique of Leymus chinensis. Acta Botanica Boreali-Occidentalia Sinica, 2010, (1): 120-125.
何学青, 胡小文, 王彦荣. 羊草种子休眠机制及破除方法研究. 西北植物学报, 2010, (1): 120-125.
[9] Wang P.Response of Leymus chinensis seedlings to sodium carbonate stress and mitigatory effect of external ABA. Acta Prataculturae Sinica, 1998, (1): 25-29.
王萍. 碳酸钠胁迫下羊草幼苗的生理效应及外源脱落酸的缓解效应. 草业学报, 1998, (1): 25-29.
[10] Liu J, Liu G S, Qi D M, et al.Effect of PEG on germination and active oxygen metabolism in wildrye (Leymus chinensis) seeds. Acta Prataculturae Sinica, 2002, (1): 59-64.
刘杰, 刘公社, 齐冬梅, 等. 聚乙二醇处理对羊草种子萌发及活性氧代谢的影响. 草业学报, 2002, (1): 59-64.
[11] Zhou C, Yang Y F.Water characteristics on salt-alkali resistance of two divergent types in experimental Leymus chinensis populations in the Songnen Plain of China. Acta Prataculturae Sinica, 2003, (1): 65-68.
周婵, 杨允菲. 松嫩平原两种趋异类型羊草实验种群对盐碱胁迫的水分响应. 草业学报, 2003, (1): 65-68.
[12] Jiao D Z, Gong M, Pan X Y, et al.Effects of various phytohormones on germination and growth of Leymus chinensis. Journal of Anhui Agricultural Sciences, 2010, (3): 1188-1190.
焦德志, 龚孟, 潘学岩, 等. 不同植物激素对羊草种子萌发和幼苗生长的影响. 安徽农业科学, 2010, (3): 1188-1190.
[13] Huang L H, Liang Z W, Ma H Y.Effects of different salts on seed germination and growth of Leymus chinensis. Journal of Agro-Environment Science, 2008, (5): 1974-1979.
黄立华, 梁正伟, 马红媛. 不同盐分对羊草种子萌发和根芽生长的影响. 农业环境科学学报, 2008, (5): 1974-1979.
[14] Gu A L, Yi J, Roman H, et al.Effects of low-temperatures on seed germination of Leymus chinensis and Pascopyrum smithii. Chinese Journal Grassland, 2005, (2): 50-54.
[15] Liu G S, Qi D M.Study of immature embryos of several species of Leymus via in vitro culture. Acta Prataculturae Sinica, 2004, (1): 70-73.
刘公社, 齐冬梅. 赖草属几种植物幼胚离体培养研究. 草业学报, 2004, (1): 70-73.
[16] Hu X W, Huang X H, Wang Y R.Hormonal and temperature regulation of seed dormancy and germination in Leymus chinensis. Plant Growth Regulation, 2012, (67): 199-207.
[17] Huang X H, Hu X W, Xu Z H, et al.Effects of exogenous hormones on the dormancy and germination of Leymus chinensis. Acta Prataculturae Sinica, 2013, (22): 183-189.
黄晓辉, 胡小文, 徐宗海, 等. 羊草种子休眠和萌发的激素调控研究. 草业学报, 2013, (22): 183-189.
[18] Bewley J D, Black M.Dormancy and the control of germination//Seeds springer. Boston: MA, 1994: 199-271.
[19] Kushiro T, Okamoto M, Nakabayashi K, et al.The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism. EMBO Journal, 2004, (23): 1647-1656.
[20] Chiwocha S D S, Cutler A J, Abrams S R, et al. The etr1-2 mutation in Arabidopsis thaliana affects the abscisic acid, auxin, cytokinin and gibberellin metabolic pathways during maintenance of seed dormancy, moist-chilling and germination. The Plant Journal, 2005, 42(1): 35-48.
[21] Lin J X, Li X Y, Zhang Z J, et al.Effect of temperature, salinity, alkalinity and their interactions on seed germination and seedling growth of Leymus chinensis. Acta Agrestia Sinca, 2011, (6): 1005-1009.
蔺吉祥, 李晓宇, 张兆军, 等. 温度与盐碱胁迫交互作用对羊草种子萌发与幼苗生长的影响. 草地学报, 2011, (6): 1005-1009.
[22] Ma H Y, Liang Z W, Kong X J, et al.Effects of salinity, temperature and their interaction on the germination percentage and seedling growth of Leymus chinensis. Acta Ecologica Sinica, 2008, 28(10): 4710-4717.
马红媛, 梁正伟, 孔祥军, 等. 盐分、温度及其互作对羊草种子发芽率和幼苗生长的影响. 生态学报, 2008, 28(10): 4710-4717.
[23] Ma H Y, Liang Z W, Wang Z C, et al.Lemmas and endosperms significantly inhibited germination of Leymus chinensis (Trin.) Tzvel. (Poaceae). Journal of Arid Environments, 2008, (72): 573-578.