Potential allelopathic effects of water extracts of three grasses on germination of their own seeds and seedling growth

doi:10.11686/cyxb2020031

Abstract

Abstract: Knowledge of the allelopathic potential of grass species is of great theoretical and practical significance for unraveling the degradation mechanisms in sown perennial pastures in alpine regions. The purpose of this study was to explore the potential allelopathic effects of Elymus nutans, Poa crymophila, and Festuca sinensis. Water extracts were prepared from whole plants and from plant powder of the perennial grass species. The effects of water extract on seed germination and seedling growth were investigated by laboratory bioassay. The whole plant extract of E. nutans significantly reduced the germination rate of E. nutans, P. crymophila and F. sinensis by 10.6, 7.0 and 21.4 percentage points (P<0.05), respectively, compared with the control. Seedling growth of the three grass species was also inhibited by the extract. The whole plant extract of P. crymophila inhibited the root length of E. nutans, P. crymophila and F. sinensis seedlings, the inhibition rates were 41.9%, 61.5% and 32.1%, respectively. The whole plant extract of F. sinensis significantly inhibited the dry weight of P. crymophila and E. nutans seedlings (P<0.05) by 51.2% and 46.4%, respectively. The powder extracts of E. nutans, P. crymophila, and F. sinensis significantly reduced (P<0.05) the germination rate, germination index, seedling height and root length of their own seeds and seedlings and those of other species. The different extracts of the three grasses had autotoxic and allelopathic effects on the germination and seedling growth of their own seeds and the those of other species, and the allelopathic effect of the powder extracts on the three grasses was stronger than that of whole plant extracts. A multivariate evaluation of the allelopathic effects of different extracts of the three grasses on their own seed germination and seedling growth of the other grass species ranked the allelopathic activity in the order: P. crymophila>E. nutans>F. sinensis. It is recommended to consider the inter-species allelopathic effect when establishing mixed seeding pasture in the Tibetan plateau area.

Key words: seeded pasture, seed germination, seedling growth, allelopathic effect

LIANG Jun, QUAN Xiao-long, ZHANG Jie-xue, SHI Hui-lan, DUAN Zhong-hua, QIAO You-ming. Potential allelopathic effects of water extracts of three grasses on germination of their own seeds and seedling growth[J]. Acta Prataculturae Sinica, 2020, 29(7): 81-89.

References

[1] Hu Z Z. The importance of artificial grassland in the development of prataculture and the control of environment in China of 21 century. Grassland and Turf, 2000, (1): 12-15.
胡自治. 人工草地在我国21世纪草业发展和环境治理中的重要意义. 草原与草坪, 2000, (1): 12-15.
[2] Wu G L, Liu Z H, Zhang L, et al. Effects of artificial grassland establishment on soil nutrients and carbon properties in a black-soil-type degraded grassland. Plant and Soil, 2010, 333(1): 469-479.
[3] Shi J J, Ma Y S, Dong Q M, et al. The selection experiment of fine forages in ‘Black Soil Type' degraded grasslands. Acta Agrestia Sinica, 2007, 15(6): 543-549, 555.
施建军, 马玉寿, 董全民, 等. “黑土型”退化草地优良牧草筛选试验. 草地学报, 2007, 15(6): 543-549, 555.
[4] Wu S N, Zhang X, Gao X X, et al. Succession dynamics of a plant community of degraded alpine meadow during the human-induced restoration process in the three rivers source region. Acta Ecologica Sinica, 2019, 39(7): 2444-2453.
武胜男, 张曦, 高晓霞, 等. 三江源区“黑土滩”型退化草地人工恢复植物群落的演替动态. 生态学报, 2019, 39(7): 2444-2453.
[5] Zhou H K, Zhao X Q, Zhao L, et al. The community characteristics and stability of the Elymus nutans artificial grassland in alpine meadow. Chinese Journal of Grassland, 2007, 29(2): 13-25.
周华坤, 赵新全, 赵亮, 等. 高山草甸垂穗披碱草人工草地群落特征及稳定性研究. 中国草地学报, 2007, 29(2): 13-25.
[6] Shi J J, Ma Y S, Dong Q M, et al. Effects of artificial regulation on production performance of perennial grass mixtures in the region of Three Rivers Sources. Acta Agrestia Sinica, 2018, 26(4): 907-916.
施建军, 马玉寿, 董全民, 等. 人工调控对三江源区多年生禾草混播草地生产性能的影响. 草地学报, 2018, 26(4): 907-916.
[7] Rice E L. Allelopathy 2nd. New York: Academic Press, 1984.
[8] Hou Y X, Song X Y, Yin Y L, et al. Allelopathic effects of allelochemicals of Ginkgo biloba leaf on fusarium wilt (Fusarium oxysporum) of hot pepper. Allelopathy Journal, 2016, 39(1): 103-114.
[9] Sitthinoi P, Lertmongkol S, Chanprasert W, et al. Allelopathic effects of jungle rice (Echinochloa colona (L.) Link) extract on seed germination and seedling growth of rice. Agriculture and Natural Resources, 2017, 51(2): 74-78.
[10] Ridenour W M, Callaway R M. The relative importance of allelopathy in interference: The effects of an invasive weed on a native bunchgrass. Oecologia, 2001, 126(3): 444-450.
[11] Viard-Crétat F, Baptist F, Hanna S F.et al. The allelopathic effects of Festuca paniculata depend on competition in subalpine grasslands. Plant Ecology, 2012, 213: 1963-1973.
[12] Zhang B C, Bai X F, Gu L H, et al. Study on allelopathy and natural degradation phenomena of artificial grassland on alpine meadow. Acta Ecologica Sinica, 1989, 9(2): 115-120.
张宝琛, 白雪芳, 顾立华, 等. 生化他感作用与高寒草甸上人工草场自然退化现象的研究. 生态学报, 1989, 9(2): 115-120.
[13] Ma R J, Wang M L, Zhao K, et al. Allelopathy of aqueous extract from Ligularia virgaurea, a dominant weed in psychro-grassland, on pasture plants. Chinese Journal of Applied Ecology, 2006, 17(5): 845-850.
马瑞君, 王明理, 赵坤, 等. 高寒草场优势杂草黄帚橐吾水浸液对牧草的化感作用. 应用生态学报, 2006, 17(5): 845-850.
[14] Shang Z H, Tang Y, Long R J. Allelopathic effect of Aconitum pendulum (Ranunculaceae) on seed germination and seedlings of five native grass species in the Tibetan Plateau. Nordic Journal of Botany, 2011, 29(4): 488-494.
[15] Liu Q, Wu C H, Peng A F, et al. Flavonolignans from Elymus natans L. and phytotoxic activities. Journal of Agriculture Food Chemistry, 2017, 65(7): 1320-1327.
[16] Liang J, Quan X L, Shi H L, et al. Allelopathic potential of Pedicularis kansuensis on five forage grasses. Acta Agrestia Sinica, 2019, 27(4): 889-897.
梁军, 全小龙, 史惠兰, 等. 甘肃马先蒿对五种禾草的化感潜力研究. 草地学报, 2019, 27(4): 889-897.
[17] Zeng R S. Review on bioassay methods for allelopathy research. Chinese Journal of Applied Ecology, 1999, 10(1): 125-128.
曾任森. 化感作用研究中的生物测定方法综述. 应用生态学报, 1999, 10(1): 125-128
[18] Williamson G B, Richardson D. Bioassays for allelopathy: Measuring treatment responses with independent controls. Journal of Chemical Ecology, 1988, 14(1): 181-187.
[19] Shen S C, Xu G F, Zhang F D, et al. Allelopathic effects of water extracts from sweet potato (Ipomoea batatas) leaves on five major farming weeds. Acta Ecologica Sinica, 2017, 37(6): 1931-1938.
申时才, 徐高峰, 张付斗, 等. 红薯叶片浸提液对5种主要农田杂草种子萌发及幼苗生长的化感作用. 生态学报, 2017, 37(6): 1931-1938.
[20] Lin W X, Kim K U, Smin D H. Rice allelopathic potential and its modes of action on barnyard grass (Echinochloa crusgalli). Allelopathy, 2000, 7(2): 215-224.
[21] Peng X. Allelopathic effects of water extracts of maize leaf on three Chinese herbal medicinal plants. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2019, 47(1): 194-200.
[22] Hu Y B, Chen J, Xiao T H, et al. Research on allelopathy of aqueous extract from Astragalus strictus. Acta Prataculturae Sinica, 2013, 22(6): 136-142.
胡远彬, 陈俊, 肖天昊, 等. 劲直黄芪水浸提液化感作用研究. 草业学报, 2013, 22(6): 136-142.
[23] Li W J, Luo K Y, Wu D, et al. Response of the native plant species Sophora davidii to the allelopathic effect of Eupatorium adenophorum. Acta Ecologica Sinica, 2017, 37(16): 5361-5367.
李苇洁, 罗开源, 吴迪, 等. 乡土植物白刺花对紫茎泽兰化感作用的响应. 生态学报, 2017, 37(16): 5361-5367.
[24] Kong C H. Problems needed attention on plant allelopathy research. Chinese Journal of Applied Ecology, 1998, 9(3): 332-336.
孔垂华. 植物化感作用研究中应注意的问题. 应用生态学报, 1998, 9(3): 332-336.
[25] Hu J, Li D Y, Zhu H S, et al. Effect of water extractions from the leaves and rhizomes of Poa pratensis on seed germination of three herbaceous ornamentals. Acta Agrestia Sinica, 2019, 27(1): 178-184.
胡婧, 李德颖, 朱慧森, 等. 草地早熟禾叶和根茎浸提液对3种花卉植物种子萌发的影响. 草地学报, 2019, 27(1): 178-184.
[26] Huang Y M, Zhang Y X, Liu Q L, et al. Research on allelopathy of aqueous extract from Tagetes patula to four garden plants. Acta Prataculturae Sinica, 2015, 24(6): 150-158.
黄玉梅, 张杨雪, 刘庆林, 等. 孔雀草水浸提液对4种园林植物化感作用的研究. 草业学报, 2015, 24(6): 150-158.
[27] Wu A P, Yu H, Gao S Q, et al. Differential belowground allelopathic effects of leaf and root of Mikania micrantha. Trees, 2009, 23(1): 11-17.