Effects of NaCl stress on proline metabolism of Achnatherum splendens seedling

Abstract

Abstract: Under varying levels of NaCl stress, the proline(Pro), pyrroline-5-carboxylate synthetase(P5CS), ornithine-oxo-acid transaminase(δ-OAT), and proline dehydragenase(ProDH) among leaves and roots of Achnatherum splendens were determined. The results showed that the proline metabolism synthesis path-way of the A. splendens seedling is mainly on the pathway of Glutamate.The proline content increased with the increase of NaCl,and the proline content in leaves and in roots were the highest that could be up to 585.77 and 303.36 μg/g FW respectively at the concentration of 500 mmol/L NaCl. The activity of δ-OAT was significantly lower than that in control(P<0.05), and declined with the increasing of the NaCl concentration. The activity of P5CS from the roots of Achnatherum splendens was significantly higher than leaves under the same concentration(P<0.05). At the concentration of 300 mmol/L NaCl,the P5CS activity in leaves and roots reached the 7.44 and 20.81 U/mg Protein respectively. The activity of ProDH in leaves were higher than in control, and could be up to 1.7 times than the control, but didn't change significantly in roots.

CLC Number:

DONG Qiu-li, XIA Fang-shan, DONG Kuan-hu. Effects of NaCl stress on proline metabolism of Achnatherum splendens seedling[J]. Acta Prataculturae Sinica, 2010, 19(5): 71-76.

References

[1] 燕丽萍, 夏阳, 梁慧敏, 等. 转BADH基因苜蓿T1代遗传稳定性和抗盐性研究[J]. 草业学报, 2009, 18(6): 65-71.
[2] 许祥明, 叶和春, 李国凤. 脯氨酸代谢与植物抗渗透胁迫的研究进展[J]. 植物学通报, 2000, 17(6): 536-542.
[3] Akihiro U, Yuko Y Y, Tetsuko T. Salt stress enhances proline utilization in the apical region of barley roots[J]. Biochemical and Biophysical Research Communications, 2007, 355: 61-66.
[4] 李玲, 余光辉, 曾富华. 水分胁迫下植物脯氨酸累积的分子机理[J]. 华南师范大学学报(自然科学版), 2003, 1: 126-134.
[5] 赵瑞雪, 程钰宏, 董宽虎, 等. 植物脯氨酸及其合成酶系研究进展[J]. 草业科学, 2008, 25(2): 90-96.
[6] 支立峰, 陈明清, 余涛, 等. p5cs转化水稻细胞系的研究[J]. 湖北师范学院学报, 2005, 25(14): 39-51.
[7] 陈吉宝, 景蕊莲, 毛新国, 等. 普通菜豆PvP5CS2基因对逆境胁迫的应答[J]. 作物学报, 2008, 34(7): 1121-1127.
[8] 徐春波, 米福贵, 王勇. 转基因冰草植株耐盐性研究[J]. 草地学报, 2006, 14(1): 20-23.
[9] Roosens N H, Bitar F A, Loenders K, et al. Overexpression of ornithine δ-arninotransferase increase proline biosynthesis and confers osmotolerance in transgenic plants[J]. Molecular Breeding, 2002, 9: 73-80.
[10] 赵福庚, 刘友良. 胁迫条件下高等植物体内脯氨酸代谢及调节的研究进展[J]. 植物学通报, 1999, 16(5): 540-546.
[11] 董社琴. 芨芨草的生物学特性与生态经济效益[J]. 科技情报开发与经济, 2004, 14(4): 190.
[12] 陶锦, 王果平, 陈韩飞, 等. 盐胁迫下芨芨草种子萌发中有机物及酶活性的变化[J]. 石河子大学学报(自然科学版), 2004, 22(6): 504-506.
[13] 王果平, 康喜亮, 陶锦, 等. 不同盐浓度对芨芨草种子萌发过程中几种生理指标的影响[J]. 干旱地区农业研究, 2006, 24(2): 139-142.
[14] 张耿, 高洪文, 王赞, 等. 偃麦草属植物苗期耐盐性指标筛选及综合评价[J]. 草业学报, 2007, 16(4): 55-61.
[15] Bates L S, Waldren R P, Teare I D. Rapid determination of free proline for water stress studies[J]. Plant Soil, 1973, 39: 205-207.
[16] Kavi Kishor P B K, Hong Z, Miao G, et al. Overexpression of 1-pyrroline-5-carboxylate synthetase increases proline over production and confers osmo-tolerance in transgenic plants[J]. Plant Physiology, 1995, 108: 1387-1394.
[17] Bradford M M. A rapid and sensitive method for the qantitation of microgram quantities of protein utilizing the principle of protein dye binding[J]. Analytic Biochemistry, 1976, 72: 248-254.
[18] Garcia-Rios M, Fujita T, Larosa P C, et al. Cloning of a polycistronic cDNA from tomato encoding γ-glutamy kinase and γ-glutamyl phosphate reductase[J]. Proceedings of the National Academy of Sciences of USA, 1997, 94: 8249-8254.
[19] Roosens N H, Thu T T, IskandarH M, et al. Isolation of theomithine-δ-aminotransferase cDNA and effect of salt stress on its expression in Arabidopsis thaliana[J]. Plant Physiology, 1998, 117: 263-271.
[20] Kim H R, Rho H W, Park J W, et al. Assay of ornithine aminotransferase with ninhydrin[J]. Ana1ytical Biochemistry, 1994, 223: 205-207
[21] Lutts S, Kinet J M, Bouharnlont J. NaCl-induced senescence in leaves of rice cultivars differing in salinity resistance[J]. Annals of Botany, 1999, 78: 389-398.
[22] 李红, 李波, 赵洪波, 等. 诱变处理苜蓿愈伤组织抗碱性的研究[J]. 草业科学, 2009, 26(7): 32-35.
[23] 张永锋, 梁正伟, 隋丽, 等. 盐碱胁迫对苗期紫花苜蓿生理特性的影响[J]. 草业学报, 2009, 18(4): 230-235.
[24] 陈托兄, 张金林, 陆妮, 等. 不同类型抗盐植物整株水平游离脯氨酸的分配[J]. 草业学报, 2006, 15(1): 36-41.
[25] 张金林, 陈托兄, 严学兵, 等. 烯效唑(S3307)对湖南稷子整株水平Na⁺、K⁺选择性和游离脯氨酸分配的影响[J]. 草业学报, 2006, 15(2): 42-47.
[26] 吴欣明, 王运琦, 刘建宁, 等. 羊茅属植物耐盐性评价及其对盐胁迫的生理反应[J]. 草业学报, 2007, 16(6): 67-73.
[27] 孟林, 尚春艳, 毛培春, 等. 偃麦草属植物种质材料苗期耐盐性综合评价[J]. 草业学报, 2009, 18(4): 67-74.
[28] 全先庆, 张渝洁, 单雷, 等. 高等植物脯氨酸代谢研究进展[J]. 生物技术通报, 2007, 1: 14-18.
[29] Delauney A J, HuC A A, Kishor P B K, et al. Cloning of ornithine-aminotransferase cDNA from Vigna aconitifolia by trans-complementation in Escherichia coli and regulation of proline biosynthesis[J]. Journal of Biological Chemistry, 1993, 268: 18673-18678.
[30] 黄诚梅, 杨丽涛, 江文, 等. 聚乙二醇胁迫对甘蔗生长前期叶片水势及脯氨酸代谢的影响[J]. 干旱地区农业研究, 2008, 26(1): 205-208.
[31] 王桂花, 米福贵, 刘娟, 等. P5CS基因在蒙农杂种冰草植株中的表达及耐盐性研究[J]. 华北农学报, 2007, 22(4): 33-36.