Polyploid induction and identification of Oncidium

Abstract

Abstract: The thin cell layers (TCLs) of protocorm like-bodies (PLBs) of Oncidium were used as explants for polyploid induction with colchicines in vitro. The technical system for ployploid chemical induction of Oncidium in vitro was established. The effects of different concentrations of colchicine induction on PLB formation and shoot regeneration from TCLs of Oncidium after different time treatments were investigated. Morphological and histological characters of lower epidermal cells of polyploid plantlet leaves were compared with diploid ones. Chromosomes of root tip cells of polyploid plantlets were identified. Colchicines greatly affected PLB formation and shoot regeneration from TCLs of Oncidium in vitro. The rate at which TCLs formed PLBs and the numbers of shoots were reduced, but the rate of polyploid plantlet production was higher in the treatments with higher concentrations of colchicines for longer times. Polyploid plants were short and stout with thick leaves. There were some differences in the structure of lower epidermal tissue and cell and stomata of diploid and polyploidy plantlets. The nuclei of polyploid cells were bigger and the number of chromosomes was doubled.

CLC Number:

CUI Guang-rong, ZHANG Zi-xue, ZHANG Cong-yu, HU Neng-bing, SUI Yi-hu, LI Jie-qin. Polyploid induction and identification of Oncidium[J]. Acta Prataculturae Sinica, 2010, 19(1): 184-190.

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