Abstract: The effects of fertilizer combinations on the cation-anion balance of alfalfa were analysed using a quadratic regression orthogonal rotating combination design. The most common cation in alfalfa was K⁺, which ranged from 2.40% to 4.01%, followed by Cl^- (0.84%-1.09%) and S^2- (0.35%-0.58%), while the lowest was Na⁺, 0.09%-0.22%. The cation-anion balance ranged from 155 to 501 mEq/kg DM. The factors that had a significant negative impact on the cation-anion balance in alfalfa were potassium sulfate and magnesium oxide. Even though the effects of ammonium chloride and superphosphate on the cation-anion balance were not significant, the interactions between ammonium chloride, superphosphate, and magnesium oxide significantly affected the balance. All of the fertilizers lowered the balance to 216.55 mEq/kg DM, which was not significant. However, the combination of fertilizers lowered the balance to 80.55 mEq/kg DM. The fertilizer combination which retained the lowest balance was 50 kg/ha potassium sulfate, 275 kg/ha ammonium chloride, 1 000 kg/ha superphosphate, and 55 kg/ha magnesium oxide.