Abstract: To develop a new variety of Chinese herbal medicine mixoploid, the seeds of diploid Lycium barbarum var. Changxuan No.1 were treated with a mixture of colchicine (C₂₂H₂₅O₆N) and DMSO at a range of temperatures for different times. The ideal inducing condition was for seeds treated intermittently with a solution of 0.05% colchicine and 2% DMSO for 48 hours at a culture temperature of 15-16℃. The rates of survival and of induced plants were 54.0% and 8.5%, respectively. Morphological and cytological observation showed that induced mutations were mixoploids with 53.3% 2n=2x=48, 35.1% 2n=4x=24 and other chromosome numbers of 22, 19, and 12 cells. A new mixoploid feeding L. barbarum cultivar Tianjing No.1 was successfully selected and bred. Compared with its diploid L. barbarum Changxuan No.1, Tianjing No.1 possesses taller plants, thicker and stronger stems, longer tender branches, larger leaf area, low selfing setting percentage, and thriving vegetative growth. Its forage was 102.8% higher than that of the check variety Ningqicai No.1. In addition, its crude protein, amino acid, LBP(L. barbarum polysaccharide) and flavone contents were higher than those of the check variety. So Tianjing No.1 has broad development prospects for Chinese herbal medicine-type forage grass.