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Acta Prataculturae Sinica ›› 2020, Vol. 29 ›› Issue (6): 144-152.DOI: 10.11686/cyxb2019502

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Physiological response of continuously cropped pea seedlings to inoculation with compound rhizobia preparations

YANG Ning1, MA Shao-ying2, MA Lei3, ZHANG Xu-hui1, WANG Na3, LI Sheng1,3,*, CHAI Qiang3,*   

  1. 1. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. Basic Experimental Educational Center of Gansu Agricultural University, Lanzhou 730070, China;
    3. Gansu Provincial Key Laboratory of Arid Land Crop Science, Lanzhou 730070, China
  • Received:2019-11-14 Revised:2020-01-21 Online:2020-06-20 Published:2020-06-20

Abstract: Continuous cropping poses a serious threat to the sustainability of agricultural production, especially in leguminous crops. In this experiment, pea varieties ‘Dingwan 8’ and ‘Longwan 6’ were inoculated with preparations of pea-rhizobium strains ‘ACCC15657’ and ‘ACCC15735’ and alfalfa-rhizobium strains ‘Da99’ were used as microbial materials. The experiment involved planting continuously cropped pea seedlings in pots, and evaluation of the effects of different rhizobial preparations on aboveground biomass, activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and concentrations of proline (Pro), soluble sugars (SS) and malondialdehyde (MDA). It was found that, compared to continuous crop planting without rhizobial inoculation (CK), in ‘Longwan 6’ seedlings, inoculation with a single rhizobium, ‘ACCC15735’, significantly reduced MDA content, while inoculation with two rhizobia, ‘ACCC15657’+‘ACCC15735’, significantly increased plant aboveground biomass, shoot Pro concentration, and SOD, POD and CAT activities. Inoculation with three rhizobia, ‘Da99’+‘ACCC15657’+‘ACCC15735’, significantly increased the soluble sugar concentration. In ‘Dingwan 8’, seedlings, compared to CK, when inoculated with the two rhizobia ‘ACCC15657’+‘ACCC15735’, the POD activity and MDA content were significantly lower, while the SOD activity and Pro concentration were significantly improved. When inoculated with three rhizobia, ‘DA99’+‘ACCC15657’+‘ACCC15735’, the aboveground biomass, the CAT activity and the soluble sugar content were significantly increased. Multivariate analysis showed that the average score under each treatment with rhizobia inoculation was higher than CK. The average value of the multivariate score was highest (indicating the strongest resilience to continuous cropping) in ‘Dingwan8’ seedlings when inoculated with the rhizobial combination ‘DA99’+‘ACCC15657’+‘ACCC15735’, and was highest in ‘Longwan 6’ seedlings when inoculated with ‘ACCC15657+ACCC15735’ was the highest. In summary, inoculation with rhizobial preparations improved the resilience of pea seedlings to the deleterious effects of continuous cropping, and the effect of inoculation with compound rhizobial preparations was superior to that of using a single rhizobium strain.

Key words: continuous cropping, pea, symbiotic rhizobium, physiological index