Effects of phosphorus application and inoculation with arbuscular mycorrhizal fungi on alfalfa yield and phosphorus use efficiency

doi:10.11686/cyxb2022251

Abstract

Abstract:

In order to investigate the effects of phosphorus application and inoculation with arbuscular mycorrhizal fungi on dry matter yield， phosphorus content and soil alkaline phosphatase activity （AKP） of alfalfa， the soil available phosphorus and total phosphorus of alfalfa under different inoculation and phosphorus application regimes were analyzed. The relationship between alfalfa dry matter yield and inoculation and phosphorus application and the relationship between yield and various other indicators were further clarified. In this experiment， a two-factor completely randomized design was adopted. Four inoculation treatments were included： Single inoculation with Funneliformis mosseaes （Fm， T₁）， Glomus etunicatum （Ge， T₂）， double inoculation （Fm×Ge， T₃） and no fungal treatment （CK， T₀）. As the second factor， four phosphorus application levels were included： P₂O₅ 0 （P₀）， 50 （P₁）， 100 （P₂）， 150 （P₃） mg·kg^-1 making a total of 16 treatments defining the fungus-phosphorus interaction. Each treatment was repeated 10 times. It was found that： 1） With the same inoculation treatment， the dry matter yield， phosphorus content of leaves， stems and roots， soil pH value and alkaline phosphatase activity of alfalfa all initially increased first and then decreased with increasing phosphorus application. All reached the maximum value in the P₂ treatment， and the yield of the alfalfa P fertilization treatment was significantly greater than the no P fertilization treatment （P<0.05）. The content of available phosphorus and total phosphorus in rhizosphere and non-rhizosphere soil increased gradually with increasing phosphorus application rate， and the agronomic efficiency of alfalfa phosphorus fertilizer showed a decreasing trend with increase in phosphorus application rate. 2） With the same inoculation treatment， dry matter yield， phosphorus content of plant leaves， stems and roots， rhizosphere and non-rhizosphere soil available phosphorus， total phosphorus content， phosphorus use efficiency， soil alkaline phosphatase activity was significantly greater than that of uninoculated treatment （P<0.05）， and the soil total phosphorus and available phosphorus contents were maximised in the T₃ treatment. The pH value of soil in the double inoculation treatment was significantly lower than that in the uninoculated treatment （P<0.05）， and reached the minimum value in the T₃ treatment. Based on these results， the phosphorus application （P₂O₅） treatment of 100 mg·kg^-1， coupled with the mixed inoculation of the two arbuscular mycorrhizal fungi could could be recommended and this treatment effectively promoted the absorption of available phosphorus in the soil by the roots of alfalfa plants， improved the efficiency of phosphorus use， and enhanced dry matter yield in alfalfa.

Key words: alfalfa, arbuscular mycorrhizal fungi （AMF）, phosphorus, phosphorus utilization efficiency, phosphatase activity

Xiao-xia AN, Ying-ying ZHANG, Chun-hui MA, Man LI, Qian-bing ZHANG. Effects of phosphorus application and inoculation with arbuscular mycorrhizal fungi on alfalfa yield and phosphorus use efficiency[J]. Acta Prataculturae Sinica, 2023, 32(6): 71-84.

Figures/Tables 9

References 35

1	Liu X S， Zhao J W， Liu J Y， et al. Water-phosphorus coupling enhances fine root turnover and dry matter yield of alfalfa under drip irrigation. Agronomy Journal， 2021， 113（5）： 4161-4175.
2	Zhang Q B， Liu J Y， Liu X S， et al. Optimizing water and phosphorus management to improve hay yield and water and phosphorus-use efficiency in alfalfa under drip irrigation. Food Science and Nutrition， 2020， 8（5）： 2406-2418.
3	Liu J Y， Liu X S， Zhang Q B， et al. Response of alfalfa growth to arbuscular mycorrhizal fungi and phosphate-solubilizing bacteria under different phosphorus application levels. AMB Express， 2020， 10（1）： 1-13.
4	Tshibangu K A， Lwalaba W L J， Kirika A B， et al. Effect of phosphorus and arbuscular mycorrhizal fungi （AMF） inoculation on growth and productivity of maize （Zea mays L.） in a tropical ferralsol. Gesunde Pflanzen， 2022， 74（1）： 159-165.
5	Suriyagoda L D B， Ryan M H， Renton M， et al. Above- and belowground interactions of grass and pasture legume species when grown together under drought and low phosphorus availability. Plant and Soil， 2011， 348（1）： 281-297.
6	Frosi G， Barros V A， Oliveira M T， et al. Increase in biomass of two woody species from a seasonal dry tropical forest in association with AMF with different phosphorus levels. Applied Soil Ecology， 2016， 102（2）： 46-52.
7	Popescu G C， Popescu M. Role of combined inoculation with arbuscular mycorrhizal fungi， as a sustainable tool， for stimulating the growth， physiological processes， and flowering performance of lavender. Sustainability， 2022， 14（2）： 951.
8	Chen M， Arato M， Borghi L， et al. Beneficial services of arbuscular mycorrhizal fungi-from ecology to application. Frontiers in Plant Science， 2018， 9（4）： 1270-1284.
9	Shrivastava P， Kumar R. Soil salinity： A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences， 2015， 22（2）： 123-131.
10	Kaschuk G， Kuyper T W， Leffelaar P A， et al. Are the rates of photosynthesis stimulated by the carbon sink strength of rhizobial and arbuscular mycorrhizal symbioses？ Soil Biology and Biochemistry， 2009， 41（6）： 1233-1244.
11	Tshibangu K A， Baert G， Lwalaba W L J， et al. Increasing of NPK fertilizer efficiency by arbuscular mycorrhiza in common bean （Phaseolus Vulgaris L.）. Gesunde Pflanzen， 2020， 72（4）： 303-310.
12	Cofre N， Becerra A G， Marro N， et al. Soybean growth and foliar phosphorus concentration mediated by arbuscular mycorrhizal fungi from soils under different no-till cropping systems. Rhizosphere， 2020， 16： 100254.
13	Lang M， Zhang C， Su W， et al. Long-term P fertilization significantly altered the diversity， composition and mycorrhizal traits of arbuscular mycorrhizal fungal communities in a wheat-maize rotation. Applied Soil Ecology， 2022， 170： 104261.
14	Meng L L， Srivastava A K， Kuča K， et al. Earthworm （Pheretima guillelmi）-mycorrhizal fungi （Funneliformis mosseae） association mediates rhizosphere responses in white clover. Applied Soil Ecology， 2022， 172： 104371.
15	Shan L W. Effects of AMF on photosynthetic characteristics and productivity of grassland plants under different treatment conditions. Harbin： Harbin Normal University， 2020.
	单立文. AMF在不同处理条件下对草地植物光合特征及其生产力影响的研究. 哈尔滨：哈尔滨师范大学， 2020.
16	Xie K Y， Sun L L， Zhang L W， et al. Effect of arbuscular mycorrhizal fungi and rhizobium inoculation on the biomass of alfalfa and smooth brome in mixed culture. Acta Agrestia Sinica， 2021， 29（1）： 182-188.
	谢开云，孙伶俐，张力文，等. 菌根真菌和根瘤菌接种对紫花苜蓿和无芒雀麦混播牧草生物量的影响. 草地学报， 2021， 29（1）： 182-188.
17	Liu J Y， Hui J F， Sun M Y， et al. Effects of phosphorus application and inoculating arbuscular mycorrhizae fungi （AMF） and phosphate solubilizing bacteria （PSB） on dry matter yield and phosphorus use efficiency of alfalfa. Transactions of the Chinese Society of Agricultural Engineering， 2020， 36（19）： 142-149.
	刘俊英，回金峰，孙梦瑶，等. 施磷水平和接种AMF与解磷细菌对苜蓿产量及磷素利用效率的影响. 农业工程学报， 2020， 36（19）： 142-149.
18	Fan J W， Du Y L， Wang B R， et al. Forage yield， soil water depletion， shoot nitrogen and phosphorus uptake and concentration， of young and old stands of alfalfa in response to nitrogen and phosphorus fertilisation in a semiarid environment. Field Crops Research， 2016， 198（11）： 247-257.
19	Lu R K. Methods of soil agricultural chemical analysis. Beijing： China Agricultural Science and Technology Press， 2000.
	鲁如坤. 土壤农业化学分析方法. 北京：中国农业科技出版社， 2000.
20	Wang Q， Bao Y， Liu X， et al. Spatio-temporal dynamics of arbuscular mycorrhizal fungi associated with glomalin-related soil protein and soil enzymes in different managed semiarid steppes. Mycorrhiza， 2014， 24（7）： 525-538.
21	Bundrett M C， Ashwath N， Jasper D A. Mycorrhizas in the kakadu region of tropical Australia. Plant and Soil， 1996， 184（1）： 173-184.
22	Xue Y F， Xia H Y， Christie P， et al. Crop acquisition of phosphorus iron and zinc from soil in cereal/legume intercropping systems： A critical review. Annals of Botany， 2016， 117（3）： 363-377.
23	Pacheco I， Ferreira R， Correia P， et al. Microbial consortium increases maize productivity and reduces grain phosphorus concentration under field conditions. Saudi Journal of Biological Sciences， 2021， 28（1）： 232-237.
24	Tran C T K， Watts-Williams S J， Smernik R J， et al. Effects of plant roots and arbuscular mycorrhizas on soil phosphorus leaching. Science of the Total Environment， 2020， 722： 137847.
25	Sun T， Li Z， Wu Q， et al. Effects of alfalfa intercropping on crop yield， water use efficiency， and overall economic benefit in the corn belt of Northeast China. Field Crops Research， 2018， 216（7）： 109-119.
26	Wang J， Liu X J， Hao F， et al. Study on nitrogen utilization characteristics of alfalfa with different nitrogen efficiency in different growth periods. Acta Agrestia Sinica， 2021， 29（11）： 2461-2469.
	王静，刘晓静，郝凤，等. 不同氮效率紫花苜蓿各生育期氮利用特征研究. 草地学报， 2021， 29（11）： 2461-2469.
27	Drevon J J. Efficacité d’utilisation du phosphore pour la fixation symbiotique de l’azote et phytases des nodules de légumineuses. Innovations Agronomiques， 2017， 60： 3-10.
28	Hu J， Lin X， Bentivenga S P， et al. Intraradical and extraradical communities of AM fungi associated with alfalfa respond differently to long-term phosphorus fertilization. Flora， 2019， 258： 151424.
29	Beauregard M S， Hamel C， St-Arnaud M. Long-term phosphorus fertilization impacts soil fungal and bacterial diversity but not AM fungal community in alfalfa. Microbial Ecology， 2010， 59（2）： 379-389.
30	Püschel D， Janoušková M， Voříšková A， et al. Arbuscular mycorrhiza stimulates biological nitrogen fixation in two Medicago spp. through improved phosphorus acquisition. Frontiers in Plant Science， 2017， 8： 390.
31	Liu S， Xu J， Huang H， et al. Changes in the mycorrhizal fungal community in host roots over five host generations under low and high phosphorus conditions. Plant and Soil， 2020， 456（1）： 27-41.
32	Cavagnaro T R， Bender S F， Asghari H R， et al. The role of arbuscular mycorrhizas in reducing soil nutrient loss. Trends in Plant Science， 2015， 20（5）： 283-290.
33	Darch T， Blackwell M S A， Chadwick D， et al. Assessment of bioavailable organic phosphorus in tropical forest soils by organic acid extraction and phosphatase hydrolysis. Geoderma， 2016， 284（8）： 93-102.
34	Gessa C E， Mimmo T， Deiana S， et al. Effect of aluminium and pH on the mobility of phosphate through a soil-root interface model. Plant and Soil， 2005， 272（1）： 301-311.
35	Tshibangu A K， Shutcha M N， Baert G， et al. Effect of soil properties on arbuscular mycorrhizae fungi （AMF） activity and assessment of some methods of common bean （Phaseolus vulgaris L.） inoculation in Lubumbashi region （DR. Congo）. Scholars Bulletin， 2020， 6（8）： 198-207.

编号 Number	处理 Treatments	NH₄H₂PO₄ （mg·pot^-1）	CN₂H₄O （mg·pot^-1）	摩西球囊霉 F. mosseae （g·pot^-1）	幼套球囊酶 G. etunicatum （g·pot^-1）
1	T₀P₀	0.0	105.3	0	0
2	T₀P₁	35.1	70.2	0	0
3	T₀P₂	70.2	35.1	0	0
4	T₀P₃	105.3	0.0	0	0
5	T₁P₀	0.0	105.3	10	0
6	T₁P₁	35.1	70.2	10	0
7	T₁P₂	70.2	35.1	10	0
8	T₁P₃	105.3	0.0	10	0
9	T₂P₀	0.0	105.3	0	10
10	T₂P₁	35.1	70.2	0	10
11	T₂P₂	70.2	35.1	0	10
12	T₂P₃	105.3	0.0	0	10
13	T₃P₀	0.0	105.3	5	5
14	T₃P₁	35.1	70.2	5	5
15	T₃P₂	70.2	35.1	5	5
16	T₃P₃	105.3	0.0	5	5

编号 Number	处理 Treatments	NH₄H₂PO₄ （mg·pot^-1）	CN₂H₄O （mg·pot^-1）	摩西球囊霉 F. mosseae （g·pot^-1）	幼套球囊酶 G. etunicatum （g·pot^-1）
1	T₀P₀	0.0	105.3	0	0
2	T₀P₁	35.1	70.2	0	0
3	T₀P₂	70.2	35.1	0	0
4	T₀P₃	105.3	0.0	0	0
5	T₁P₀	0.0	105.3	10	0
6	T₁P₁	35.1	70.2	10	0
7	T₁P₂	70.2	35.1	10	0
8	T₁P₃	105.3	0.0	10	0
9	T₂P₀	0.0	105.3	0	10
10	T₂P₁	35.1	70.2	0	10
11	T₂P₂	70.2	35.1	0	10
12	T₂P₃	105.3	0.0	0	10
13	T₃P₀	0.0	105.3	5	5
14	T₃P₁	35.1	70.2	5	5
15	T₃P₂	70.2	35.1	5	5
16	T₃P₃	105.3	0.0	5	5

处理 Treatment	第1茬 First cut	第2茬 Second cut	第3茬 Third cut
T₀P₀	14.03±0.95Cc	16.22±0.70Bc	11.63±0.92Db
T₀P₁	21.73±0.60Ba	16.50±1.22Cc	14.57±0.51Ca
T₀P₂	18.73±0.81Db	21.67±0.51Ca	14.47±0.55Ca
T₀P₃	21.57±0.61Ba	18.13±0.90Bb	13.43±0.55Ca
T₁P₀	17.73±0.85ABc	17.05±0.78Bd	15.23±0.40Bc
T₁P₁	22.95±0.84Bb	21.67±0.51ABb	15.78±0.62Bc
T₁P₂	25.90±0.80Ba	23.85±0.65Ba	18.65±0.56ABa
T₁P₃	23.07±0.87Ab	19.30±0.69ABc	17.35±0.65ABb
T₂P₀	16.63±0.61Bc	16.89±0.64Bd	13.93±0.85Cd
T₂P₁	21.80±0.50Bb	20.50±1.06Bb	15.03±0.90BCc
T₂P₂	23.43±0.61Ca	21.90±0.72Ca	18.10±0.80Ba
T₂P₃	22.57±0.76ABab	18.17±0.76Bc	16.32±0.51Bb
T₃P₀	19.03±0.70Ac	19.00±0.66Ac	16.77±0.58Ab
T₃P₁	24.38±0.94Ab	22.47±0.55Ab	17.30±0.72Ab
T₃P₂	27.23±0.83Aa	25.87±0.68Aa	19.45±0.56Aa
T₃P₃	23.70±0.79Ab	20.13±0.75Ac	17.58±0.45Ab
Fungus treatment （T）	**	**	**
Phosphorus treatment （P）	**	**	**
Fungus phosphorus interaction （T×P）	**	**	**

处理 Treatment	第1茬 First cut	第2茬 Second cut	第3茬 Third cut
T₀P₀	14.03±0.95Cc	16.22±0.70Bc	11.63±0.92Db
T₀P₁	21.73±0.60Ba	16.50±1.22Cc	14.57±0.51Ca
T₀P₂	18.73±0.81Db	21.67±0.51Ca	14.47±0.55Ca
T₀P₃	21.57±0.61Ba	18.13±0.90Bb	13.43±0.55Ca
T₁P₀	17.73±0.85ABc	17.05±0.78Bd	15.23±0.40Bc
T₁P₁	22.95±0.84Bb	21.67±0.51ABb	15.78±0.62Bc
T₁P₂	25.90±0.80Ba	23.85±0.65Ba	18.65±0.56ABa
T₁P₃	23.07±0.87Ab	19.30±0.69ABc	17.35±0.65ABb
T₂P₀	16.63±0.61Bc	16.89±0.64Bd	13.93±0.85Cd
T₂P₁	21.80±0.50Bb	20.50±1.06Bb	15.03±0.90BCc
T₂P₂	23.43±0.61Ca	21.90±0.72Ca	18.10±0.80Ba
T₂P₃	22.57±0.76ABab	18.17±0.76Bc	16.32±0.51Bb
T₃P₀	19.03±0.70Ac	19.00±0.66Ac	16.77±0.58Ab
T₃P₁	24.38±0.94Ab	22.47±0.55Ab	17.30±0.72Ab
T₃P₂	27.23±0.83Aa	25.87±0.68Aa	19.45±0.56Aa
T₃P₃	23.70±0.79Ab	20.13±0.75Ac	17.58±0.45Ab
Fungus treatment （T）	**	**	**
Phosphorus treatment （P）	**	**	**
Fungus phosphorus interaction （T×P）	**	**	**

处理 Treatment	苜蓿叶片磷含量 Phosphorus content in alfalfa leaves （mg·kg^-1）			苜蓿茎磷含量 Phosphorus content in alfalfa stem （mg·kg^-1）
处理 Treatment	第1茬First cut	第2茬Second cut	第3茬Third cut	第1茬First cut	第2茬Second cut	第3茬Third cut
T₀P₀	0.203±0.008Ba	0.252±0.007Cb	0.219±0.006Bb	0.155±0.003Abc	0.146±0.009Bc	0.111±0.003Bb
T₀P₁	0.210±0.005Ba	0.270±0.008Db	0.284±0.014Ba	0.175±0.002Ba	0.182±0.003Cb	0.141±0.013BCa
T₀P₂	0.207±0.008Da	0.388±0.007Ba	0.277±0.002Ba	0.167±0.008Cab	0.216±0.010Ba	0.151±0.003Ca
T₀P₃	0.204±0.009Ca	0.383±0.018Ba	0.233±0.008Cb	0.150±0.007Cc	0.207±0.001Ba	0.143±0.003Ca
T₁P₀	0.216±0.002ABb	0.297±0.001Bc	0.233±0.006Bb	0.161±0.008Ac	0.184±0.003Ac	0.119±0.005Bc
T₁P₁	0.254±0.009Aa	0.350±0.006Cb	0.240±0.007Cb	0.169±0.008Bbc	0.216±0.004ABb	0.149±0.005Bb
T₁P₂	0.232±0.002Cb	0.378±0.015Ba	0.288±0.007Ba	0.193±0.010ABa	0.237±0.006Aa	0.190±0.007Ba
T₁P₃	0.226±0.011Bb	0.377±0.011Ba	0.281±0.006Ba	0.179±0.005Bab	0.219±0.006ABb	0.186±0.005Aa
T₂P₀	0.209±0.004ABb	0.286±0.013Bc	0.223±0.009Bc	0.153±0.007Ab	0.196±0.001Ac	0.114±0.002Bc
T₂P₁	0.245±0.010Aa	0.374±0.019Bb	0.249±0.004Cb	0.176±0.009Ba	0.208±0.008Bbc	0.134±0.003Cb
T₂P₂	0.255±0.002Ba	0.389±0.001ABa	0.271±0.011Ba	0.191±0.004Ba	0.232±0.009Aa	0.156±0.004Ca
T₂P₃	0.254±0.008Aa	0.368±0.016Bb	0.266±0.011Ba	0.178±0.009Ba	0.211±0.002Bb	0.152±0.008Ca
T₃P₀	0.225±0.009Ab	0.328±0.003Ab	0.277±0.007Ac	0.164±0.005Ac	0.199±0.004Ac	0.146±0.003Ac
T₃P₁	0.241±0.008Ab	0.391±0.016Aa	0.304±0.005Ab	0.197±0.004Ab	0.228±0.003Aab	0.167±0.007Ab
T₃P₂	0.292±0.006Aa	0.399±0.001Aa	0.333±0.008Aa	0.220±0.010Aab	0.216±0.007Bb	0.207±0.001Aa
T₃P₃	0.231±0.007Bb	0.389±0.014Aa	0.325±0.006Aa	0.207±0.007Aa	0.230±0.007Aa	0.172±0.006Bb
T	**	**	**	**	**	**
P	**	**	**	**	**	**
T×P	**	**	**	**	**	**
处理 Treatment	苜蓿根部磷含量 Phosphorus content in alfalfa roots （mg·kg^-1）			磷肥农学效率 Agronomic efficiency of phosphate fertilizer （%）
处理 Treatment	第1茬First cut	第2茬Second cut	第3茬Third cut	磷肥农学效率 Agronomic efficiency of phosphate fertilizer （%）
T₀P₀	0.173±0.006Bc	0.182±0.003Cc	0.144±0.003Cb	—
T₀P₁	0.224±0.006Aa	0.216±0.008Cb	0.178±0.001Ba	24.26±1.20Aa
T₀P₂	0.198±0.006Cb	0.245±0.006Ca	0.170±0.004Da	14.43±0.70Bb
T₀P₃	0.190±0.002Db	0.231±0.010Ca	0.172±0.006Ca	8.33±0.41Ac
T₁P₀	0.201±0.008Ac	0.217±0.006Bc	0.151±0.003Cd	—
T₁P₁	0.216±0.004Ab	0.265±0.009ABa	0.170±0.003Bc	23.07±1.67Aa
T₁P₂	0.240±0.009ABa	0.271±0.001Ba	0.181±0.004Cb	20.43±1.08Aa
T₁P₃	0.222±0.004Bb	0.246±0.004Bb	0.196±0.003Ba	7.19±0.39Ab
T₂P₀	0.204±0.004Ab	0.212±0.005Bb	0.162±0.004Bc	—
T₂P₁	0.214±0.006Ab	0.253±0.005Ba	0.175±0.004Bb	21.96±1.13Aa
T₂P₂	0.234±0.006Ba	0.267±0.010Ba	0.199±0.007Ba	17.76±0.85ABb
T₂P₃	0.205±0.004Cb	0.255±0.007Ba	0.180±0.006Cb	7.11±0.37Ac
T₃P₀	0.212±0.007Ac	0.243±0.004Ac	0.188±0.003Ac	—
T₃P₁	0.227±0.004Ab	0.273±0.006Ab	0.215±0.008Ab	20.78±1.07Aa
T₃P₂	0.253±0.006Aa	0.304±0.004Aa	0.233±0.003Aa	19.72±0.77Aa
T₃P₃	0.241±0.012Aa	0.295±0.013Aa	0.219±0.006Ab	4.90±0.31Ab
T	**	**	**	NS
P	**	**	**	**
T×P	**	NS	**	*