Effects of nitrogen and phosphorus addition on litter decomposition in Hulunber steppe

doi:10.11686/cyxb2022038

Abstract

Abstract:

Litter decomposition is an important component process in grassland ecosystem energy flows and cycling of carbon （C）， nitrogen （N）， phosphorus （P） and other elements. Litter decomposition rate depends on its initial chemical composition （internal factors） and soil environment （external factors）. N and P as essential macronutrients for plants， are important to regulation of internal and external factors in litter decomposition. Here we report a two-year N and P controlled addition experiment with two N levels （0， 10 g N·m^-2·yr^-1） and 2 P levels （0， 10 g N·m^-2·yr^-1） in their four combinations to define the N×P interaction. There are five replicates. The experiment studied Leymus chinensis， Cleistogenes squarrosa and Thermopsis lanceolata， which have different nutrient utilization patterns and used the net-bag decomposition method to study the effects of N and P addition on litter decomposition. It was found that the litter decomposition process was affected by both internal factors and external factors， with the external factors having a greater effect than the internal factors. In the external factors， nutrient addition promoted the decomposition of all three litter types. With respect to the internal factors， nutrient addition promoted the degradation of C. Squarrosa and T. lanceolata， but inhibited the degradation of L. chinensis. The rate of release of N and P was positively correlated with the contents of N and P in the initial litter and soil environment. This study revealed that the differences of nitrogen and phosphorus nutrient in soil were the main factors affecting litter decomposition and nutrient release. In future studies， more attention should be paid to the influence of external factors on the litter decomposition process.

Key words: meadow steppe, nitrogen and phosphorus addition, litter decomposition, nutrient release, decomposition rate

Peng-chong DU, Yu-zhen PAN, Shuang-li HOU, Zhi-hui WANG, Hong-yi WANG. Effects of nitrogen and phosphorus addition on litter decomposition in Hulunber steppe[J]. Acta Prataculturae Sinica, 2023, 32(2): 44-53.

Figures/Tables 6

References 43

1	Carrera A L， Bertiller M B．Combined effects of leaf litter and soil microsite on decomposition process in arid rangelands. Journal of Environmental Management， 2013， 114（15）： 505-511.
2	Li H， Chen W M， Chen X， et al. Poisonous weed species and their harmfulness in the grassland of Yili regions， Xinjiang Uygur Autonomous Region of China. Pratacultural Science， 2010， 27（11）： 171-173.
	李宏，陈卫民，陈翔，等. 新疆伊犁草原毒害草种类及其发生与危害. 草业科学， 2010， 27（11）： 171-173.
3	Garibald L A， Semmartin M， Chaneton E J. Grazing-induced changes in plant composition affect litter quality and nutrient cycling in flooding Pampa grasslands. Oecologia， 2017， 151（4）： 650-662.
4	Wang Z H， Wang Z R， Li T P， et al. N and P fertilization enhanced carbon decomposition function by shifting microbes towards an r-selected community in meadow grassland soils. Ecological Indicators， 2021， 132： 108306.
5	Chen J， Li Y， Huang J H. Decomposition of mixed litter of four dominant species in an Inner Mongolia steppe. Chinese Journal of Plant Ecology， 2011， 35（1）： 9-16.
	陈瑾，李扬，黄建辉. 内蒙古典型草原4种优势植物凋落物的混合分解研究. 植物生态学报， 2011， 35（1）： 9-16.
6	Yang L L， Gong J R， Liu M， et al. Advances in the effect of nitrogen deposition on grassland litter decomposition. Chinese Journal of Plant Ecology， 2017， 41（8）： 894-913.
	杨丽丽，龚吉蕊，刘敏，等. 氮沉降对草地凋落物分解的影响研究进展. 植物生态学报， 2017， 41（8）： 894-913.
7	Zhao H M， Huang G， Ma J， et al. Study on dynamic status of litter decomposition and nutrients of typical desert plants. Arid Zone Research， 2012， 29（4）： 628-634.
	赵红梅，黄刚，马健，等. 典型荒漠植物凋落物分解及养分动态研究. 干旱区研究， 2012， 29（4）： 628-634.
8	Hou S L， Hättenschwiler S， Yang J J， et al. Increasing rates of long-term nitrogen deposition consistently increased litter decomposition in a semi-arid grassland. New Phytologist， 2021， 229： 296-307.
9	Wang M M， Hou F J. Influence of main factors on grass litter decomposition. Pratacultural Science， 2012， 29（10）： 1631-1637.
	王苗苗，侯扶江. 草地凋落物分解的主要影响因素. 草业科学， 2012， 29（10）： 1631-1637.
10	Zhang Y H， Feng J C， Isbell F， et al. Productivity depends more on the rate than the frequency of N addition in a temperate grassland. Scientific Reports， 2015， 5： 12558.
11	Peñuelas J， Poulter B， Sardans J， et al. Human-induced nitrogen-phosphorus imbalances alter natural and managed ecosystems across the globe. Nature Communications， 2013， 4： 2934.
12	Zhan S X， Wang Y， Zhu Z C， et al. Nitrogen enrichment alters plant N∶P stoichiometry and intensifies phosphorus limitation in a steppe ecosystem. Environmental and Experimental Botany， 2017， 134： 21-32.
13	Berg B， Staaf H. Decompostion rate and chemical changes in decomposing needle litter of Scotspine Ⅱ. Influence of chemical composition. Ecological Bulletins-NFR， 1980， 32： 373-390.
14	Keeler B L， Hobbie S E， Kellogg L E. Effects of long-term nitrogen addition on microbial enzyme activity in eight forested and grassland sites： Implications for litter and soil organic matter decomposition. Ecosystems， 2009， 12（1）： 1-15.
15	Galloway J N， Townsend A R， Erisman J W， et al. Transformation of the nitrogen cycle： Recent trends， questions and potential solutions. Science， 2008， 320（5）： 889-892.
16	Hou S L， Lv X T. Mixing effects of litter decomposition at plant organ and species levels in a temperate grassland. Plant Soil， 2021， 459： 387-396.
17	Dukes J S， Field C B. Diverse mechanisms for CO₂ effects on grassland litter decomposition. Global Change Biology， 2000， 6（6）： 145-154.
18	Michs P， Aber J D， Boone R D， et al. Short-term soil respiration and nitrogen immobilization response to nitrogen applications in contron and nitrogen-enriched temperate forests. Forest Ecology & Management， 2004， 196（1）： 57-70.
19	Wang H Y， Chang J F， Wang Z W. Responses of community species diversity and productivity to nitrogen and phosphorus addition during restoration of degraded grassland. Scientia Agricultura Sinica， 2020， 53（13）： 2604-2613.
	王洪义，常继方，王正文. 退化草地恢复过程中群落物种多样性及生产力对氮磷养分的响应. 中国农业科学， 2020， 53（13）： 2604-2613.
20	Bao S D. Soil and agricultural chemistry analysis. Beijing： China Agriculture Press， 2013.
	鲍士旦. 土壤农化分析. 北京：中国农业出版社， 2013.
21	Wood T G. Field investigations on the decomposition of leaves of Eucalyptus delegatensis in relation to environmental factors. Pedobiology， 1991， 14： 343-371.
22	Li W Y， Qiu X， Bai L， et al. Effects of nitrogen and phosphorus addition on litter decomposition on the Stipa baicalensis steppe. Acta Prataculturae Sinica， 2017， 26（8）： 43-53.
	李文亚，邱璇，白龙，等. 氮、磷添加对贝加尔针茅草原凋落物分解的影响. 草业学报， 2017， 26（8）： 43-53.
23	Gong X J. The response of litter decomposition to carbon， nitrogen and phosphorus additions for four typical tree species in mid-subtropical zone. Nanchang： Nanchang University， 2011.
	弓晓静. 中亚热带四种树种凋落叶分解对碳氮磷调控的响应. 南昌：南昌大学， 2011.
24	He Y T， Qi Y C， Dong Y S， et al. Advances in the influence of external nitrogen input on soil microbiological characteristics of grassland Ecosystem. Advances in Earth Science， 2010， 25（8）： 877-885.
	何亚婷，齐玉春，董云社，等. 外源氮输入对草地土壤微生物特性影响的研究进展. 地球科学进展， 2010， 25（8）： 877-885.
25	Olander L P， Vitousek P M. Regulation of soil phosphatase and chitinase activity by N and P availability. Biogeochemistry， 2000， 49（2）： 175-191.
26	Berg B， McClaugherty C. Plant litter： Decomposition， humus formation， carbon sequestration. Berlin： Springer， 2003.
27	Song X Z， Jiang H， Ma Y D. Litter decomposition across climate zone in Eastern China： The integrated influence of climate and litter quality. Acta Ecologica Sinica， 2009， 29（10）： 5219-5226.
	宋新章，江洪，马元丹. 中国东部气候带凋落物分解特征-气候和基质质量的综合影响. 生态学报， 2009， 29（10）： 5219-5226.
28	Wen H Y， Fu H， Guo D. Influence of nitrogen addition on Stipa bungeana and Heteropappus altaicus litter decomposition and nutrient release in a steppe located on the Loess Plateau. Acta Ecologica Sinica， 2017， 37（6）： 2014-2022.
	文海燕，傅华，郭丁. 黄土高原典型草原优势植物凋落物分解及养分释放对氮添加的响应. 生态学报， 2017， 37（6）： 2014-2022.
29	Sarah E， Hobbie， Laura G. Litter decomposition in moist acidic and non-acidic tundra with different glacial histories. Oecologia， 2004， 140（1）： 113-124.
30	Liu H L， Li X L， Liang Y M. Effects of raising soil fertilities by planting different forage sepcies on newly-built terrence land on China loess hilly regions. Acta Botanica Boreali-Occidentalia Sinica， 1998， 18（2）： 287-291.
	刘洪岭，李香兰，梁一民. 禾本科及豆科牧草对黄土丘陵区台田土壤培肥效果的比较研究. 西北植物学报， 1998， 18（2）： 287-291.
31	Harpole W S， Tilman D. Grassland species loss resulting from reduce niche dimension. Nature， 2007， 446： 791-793.
32	Hobbie S E. Interactions between litter lignin and soil nitrogen availability during leaf litter decomposition in a Hawaiian montane forest. Ecosystems， 2000， 3： 484-494.
33	Ye R H， Shan Y M， Zhang P J， et al. Effects of nitrogen and water addition on litter decomposition in desert grassland under different grazing intensities. Acta Ecologica Sinica， 2019， 31（11）： 3631-3638.
	晔薷罕，单玉梅，张璞进，等. 荒漠草原不同放牧强度背景下添加氮水对凋落物分解的影响. 生态学报， 2019， 31（11）： 3631-3638.
34	Wang H Y， Wang Z W， Ding R， et al. The impacts of nitrogen deposition on community N∶P stoichiometry do not depend on phosphorus availability in a temperate meadow steppe. Environmental Pollution， 2018， 242： 82-89.
35	Berg B. Litter decomposition and organic matter turnover in northern forest soils. Forest Ecology and Management， 2000， 133： 13-22.
36	Kondo R， Iimori T， Imamura H， et al. Polymerization of DHP and depolymerization of DHP-glucoside by lignin oxidizing enzymes. Biotechnology， 1990， 13： 181-188.
37	Zhao Y T， Li X F， Han S J， et al. Soil enzyme activities under two forest types as affected by different levels of nitrogen deposition. Chinese Journal of Applied Ecology， 2008， 19（12）： 2769-2773.
	赵玉涛，李雪峰，韩士杰，等. 不同氮沉降水平下两种林型的主要土壤酶活性. 应用生态学报， 2008， 19（12）： 2769-2773.
38	Ge X M， Wu L， Tang L Z. Review on research progress of relationships between enzyme and litter decomposition. World Forestry Research， 2013， 26（1）： 43-47.
	葛晓敏，吴麟，唐罗忠. 森林凋落物分解与酶的相互关系的研究进展. 世界林业研究， 2013， 26（1）： 43-47.
39	Cornwell W K， Cornelissen J H C， Amatangelo K， et al. Plant species traits are the predominant control on litter decomposition rates within biomes worldwide. Ecology Letters， 2008， 11（10）： 1065-1071.
40	Chen Y， Wang F M， Mo Q F， et al. Effects of nitrogen and phosphorus additions on woody debris decomposition in a secondary tropical forest of South China. Chinese Journal Applied Environmental Biology， 2015， 21（4）： 747-753.
	陈瑶，王法明，莫其锋，等. 氮磷添加对华南热带森林尾叶桉木质残体分解和养分动态的影响. 应用与环境生物学报， 2015， 21（4）： 747-753.
41	Liao L P， Gao H， Wang S L， et al. The effect of nitrogen addition on soil nutrient leaching and the decomposition of Chinese fir leaf litter. Chinese Journal of Plant Ecology， 2000， 24（1）： 34-39.
	廖利平，高洪，汪思龙，等. 外加氮源对杉木叶凋落物分解及土壤养分淋失的影响. 植物生态学报， 2000， 24（1）： 34-39.
42	Berg B， Matzer E. Effect to N deposition on decomposition of plant litter and soil organic matter in forest ecosystems. Enviornmental Reviews， 1997， 5（1）： 1-25.
43	Yang J S， Liu J S， Yu J B， et al. Decomposition and nutrient dynamics of marsh litter in the Sanjiang Plain， China. Acta Ecologica Sinica， 2006， 26（5）： 1207-1212.
	杨继松，刘景双，于君宝，等. 三江平原沼泽湿地枯落物分解及营养动态. 生态学报， 2006， 26（5）： 1207-1212.

凋落物种类 Litter species	养分添加 Nutrient addition	碳 Carbon （g·kg^-1）	氮 Nitrogen （g·kg^-1）	磷 Phosphorus （g·kg^-1）	碳氮比 C/N
羊草 L. chinensis	CK	305.0±4.5a	10.7±0.1b	1.08±0.11c	28.5±4.5ab
	N	296.0±8.5a	14.5±2.2a	0.98±0.08c	20.8±8.5c
	P	278.6±9.1b	9.4±0.3c	3.18±0.04b	29.6±9.1a
	NP	264.6±0.3c	13.8±0.1a	3.90±0.26a	22.4±0.2bc
糙隐子草 C. squarrosa	CK	221.5±4.4a	10.1±0.5bc	1.54±0.04c	21.9±0.6a
	N	199.1±8.9b	11.4±1.0ab	0.76±0.10d	17.5±0.6b
	P	199.1±10.9b	9.1±0.9c	3.70±0.28a	22.1±1.1a
	NP	190.0±14.8b	12.2±0.2a	2.80±0.03b	20.9±1.4a
披针叶黄华 T. lanceolata	CK	252.5±4.2a	18.0±0.1b	1.26±0.03b	14.0±1.3ab
	N	259.3±8.1a	21.1±0.1a	1.18±0.09b	12.3±0.3b
	P	223.7±0.1b	15.4±1.2c	1.27±0.02b	14.6±0.1a
	NP	208.1±2.0c	15.9±0.9c	1.64±0.01a	13.1±0.6b

凋落物种类 Litter species	养分添加 Nutrient addition	碳 Carbon （g·kg^-1）	氮 Nitrogen （g·kg^-1）	磷 Phosphorus （g·kg^-1）	碳氮比 C/N
羊草 L. chinensis	CK	305.0±4.5a	10.7±0.1b	1.08±0.11c	28.5±4.5ab
	N	296.0±8.5a	14.5±2.2a	0.98±0.08c	20.8±8.5c
	P	278.6±9.1b	9.4±0.3c	3.18±0.04b	29.6±9.1a
	NP	264.6±0.3c	13.8±0.1a	3.90±0.26a	22.4±0.2bc
糙隐子草 C. squarrosa	CK	221.5±4.4a	10.1±0.5bc	1.54±0.04c	21.9±0.6a
	N	199.1±8.9b	11.4±1.0ab	0.76±0.10d	17.5±0.6b
	P	199.1±10.9b	9.1±0.9c	3.70±0.28a	22.1±1.1a
	NP	190.0±14.8b	12.2±0.2a	2.80±0.03b	20.9±1.4a
披针叶黄华 T. lanceolata	CK	252.5±4.2a	18.0±0.1b	1.26±0.03b	14.0±1.3ab
	N	259.3±8.1a	21.1±0.1a	1.18±0.09b	12.3±0.3b
	P	223.7±0.1b	15.4±1.2c	1.27±0.02b	14.6±0.1a
	NP	208.1±2.0c	15.9±0.9c	1.64±0.01a	13.1±0.6b

凋落物种类 Litter species	分解位点 Decomposition site	养分添加 Nutrient addition	回归方程 Equation	分解系数 Decomposition coefficient	半分解时间 Half-decaying time （yr）	分解95%时间 95% decaying time （yr）
羊草 L. chinensis	样方内 Inside quadrat	CK	y=e^-0.303^t	0.303	2.28	9.87
		N	y=e^-0.356^t	0.356	1.94	8.40
		P	y=e^-0.362^t	0.362	1.91	8.25
		NP	y=e^-0.403^t	0.403	1.71	7.41
	样方外 Outside quadrat	CK	y=e^-0.300^t	0.300	2.34	10.10
		N	y=e^-0.288^t	0.288	2.40	11.31
		P	y=e^-0.321^t	0.321	2.16	9.33
		NP	y=e^-0.273^t	0.273	2.53	10.94
糙隐子草 C. squarrosa	样方内 Inside quadrat	CK	y=e^-0.412^t	0.412	1.68	7.26
		N	y=e^-0.454^t	0.454	1.52	6.59
		P	y=e^-0.469^t	0.469	1.47	6.38
		NP	y=e^-0.487^t	0.487	1.42	6.14
	样方外 Outside quadrat	CK	y=e^-0.405^t	0.405	1.71	7.38
		N	y=e^-0.421^t	0.421	1.65	7.12
		P	y=e^-0.423^t	0.423	1.63	7.08
		NP	y=e^-0.432^t	0.432	1.60	6.92
披针叶黄华 T. lanceolata	样方内 Inside quadrat	CK	y=e^-0.427^t	0.427	1.62	7.01
		N	y=e^-0.476^t	0.476	1.45	6.29
		P	y=e^-0.504^t	0.504	1.37	5.94
		NP	y=e^-0.533^t	0.533	1.30	5.61
	样方外 Outside quadrat	CK	y=e^-0.433^t	0.433	1.60	6.91
		N	y=e^-0.444^t	0.444	1.56	6.74
		P	y=e^-0.499^t	0.499	1.38	6.01
		NP	y=e^-0.464^t	0.464	1.49	6.45

凋落物种类 Litter species	分解位点 Decomposition site	养分添加 Nutrient addition	回归方程 Equation	分解系数 Decomposition coefficient	半分解时间 Half-decaying time （yr）	分解95%时间 95% decaying time （yr）
羊草 L. chinensis	样方内 Inside quadrat	CK	y=e^-0.303^t	0.303	2.28	9.87
		N	y=e^-0.356^t	0.356	1.94	8.40
		P	y=e^-0.362^t	0.362	1.91	8.25
		NP	y=e^-0.403^t	0.403	1.71	7.41
	样方外 Outside quadrat	CK	y=e^-0.300^t	0.300	2.34	10.10
		N	y=e^-0.288^t	0.288	2.40	11.31
		P	y=e^-0.321^t	0.321	2.16	9.33
		NP	y=e^-0.273^t	0.273	2.53	10.94
糙隐子草 C. squarrosa	样方内 Inside quadrat	CK	y=e^-0.412^t	0.412	1.68	7.26
		N	y=e^-0.454^t	0.454	1.52	6.59
		P	y=e^-0.469^t	0.469	1.47	6.38
		NP	y=e^-0.487^t	0.487	1.42	6.14
	样方外 Outside quadrat	CK	y=e^-0.405^t	0.405	1.71	7.38
		N	y=e^-0.421^t	0.421	1.65	7.12
		P	y=e^-0.423^t	0.423	1.63	7.08
		NP	y=e^-0.432^t	0.432	1.60	6.92
披针叶黄华 T. lanceolata	样方内 Inside quadrat	CK	y=e^-0.427^t	0.427	1.62	7.01
		N	y=e^-0.476^t	0.476	1.45	6.29
		P	y=e^-0.504^t	0.504	1.37	5.94
		NP	y=e^-0.533^t	0.533	1.30	5.61
	样方外 Outside quadrat	CK	y=e^-0.433^t	0.433	1.60	6.91
		N	y=e^-0.444^t	0.444	1.56	6.74
		P	y=e^-0.499^t	0.499	1.38	6.01
		NP	y=e^-0.464^t	0.464	1.49	6.45

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[10]	LIU Jing, XIE Wan-yu, ZHANG Qiao-ming, XU Shao-jun. Leaf decomposition and nutrient release characteristics of different plant species in the Loess Hilly region [J]. Acta Prataculturae Sinica, 2018, 27(9): 25-33.
[11]	LUO Yong-qing, YUE Xiang-fei, LI Yu-qiang, ZHAO Xue-yong, DING Jie-ping, YAN Zhi-qiang. Effect of precipitation patterns on litter decomposition in three annual herb species in the Horqin Sandy Land [J]. Acta Prataculturae Sinica, 2018, 27(2): 206-212.
[12]	LI Wen-Ya, QIU Xuan, BAI Long, YANG Dian-Lin. Effects of nitrogen and phosphorus addition on litter decomposition on the Stipa baicalensis steppe [J]. Acta Prataculturae Sinica, 2017, 26(8): 43-53.
[13]	NA Ya, SUN Qi-Zhong, WANG Hong-Mei. Fatty acid characteristics of forage silages from the Hulunbeir Meadow Steppe [J]. Acta Prataculturae Sinica, 2017, 26(2): 215-223.
[14]	HU Fei-Long, YAN Yan, LU Xiao-Qiang, WU Jun, DING Hui, LIU Zhi-Min. Biomass allocation patterns in the temperate meadow steppe in Inner Mongolia [J]. Acta Prataculturae Sinica, 2016, 25(4): 36-44.
[15]	LIU Jia, ZHANG Jie, QIN Wen-Jing, YANG Cheng-Chun, XIE Jie, XIANG Xing-Jia, CAO Wei-Dong, XU Chang-Xu. Decomposition and nutrient release characteristics of different Vicia villosa green manure applications in red soil uplands of South China [J]. Acta Prataculturae Sinica, 2016, 25(10): 66-76.