控水处理对紫花苜蓿抗寒性影响的代谢组学分析

doi:10.11686/cyxb2018756

摘要/Abstract

摘要： 为研究控水处理对紫花苜蓿抗寒性影响的机制,本研究设置了80%和25%最大持水量[充分浇水(WW)和水分亏缺(WD)]两种水分处理,对比了处理后的苜蓿分别在常温和低温环境中的抗寒性(LT₅₀),并采用LC-MS技术分析了在试验过程中根颈内代谢物的变化。结果显示,无论是在常温还是在低温环境下,WD处理苜蓿的抗寒性都要优于WW处理的,控水处理可以提高苜蓿的抗寒性。在对渗透调节物质可溶性糖含量的分析中,WD处理中苜蓿根颈细胞内可溶性糖的含量显著高于WW处理中的。代谢组分析发现,与WW处理相比,WD控水处理提高了甘油磷脂类和脂肪酰基类物质的含量,而它们不仅是细胞膜的组成成分,还涉及到低温胁环境下细胞膜的流动性和稳定性,这对提高苜蓿抗寒性起到非常重要的作用。此外,糖类物质也是在京都基因与基因组百科全书富集分析中参与显著代谢通路的物质,鉴于可溶性糖渗透调节和冷冻保护剂的作用,控水处理下D-麦芽糖含量的上调,可能也是导致苜蓿抗寒性提高的原因。

关键词: 紫花苜蓿, 水分胁迫, 抗寒性, 代谢组, LC-MS

Abstract: This research examined the influence of water deficit on alfalfa freezing tolerance. Two treatments with alfalfa plants grown at 80% and 25% soil water holding capacity were set up, and the semilethal temperature (LT₅₀) determined and compared for the contrasting watering regimes. Metabolite levels in alfalfa crowns were analyzed using an LC-MS instrument. Results showed the freezing tolerance of alfalfa plants in water deficit (WD) to be superior to that of well-watered plants (WW), with higher total soluble sugar levels WD than WW. Compared with the WW treatment, WD plants displayed up-regulated glycerophospholipids and fatty acyls which are the main constituents of the plasma membrane and have a function of enhancing membrane fluidity and stability. Hence, the up-regulated compounds are very important to the capability to resist cold damage. In addition, from KEGG analysis of carbohydrate profiles of the two treatments, the up-regulation of D-maltose levels in WD plants appears to be another important contributing factor to the enhanced freezing tolerance in the WD plants.

Key words: alfalfa, water deficit, freezing tolerance, metabolomics, LC-MS

徐洪雨, 李向林. 控水处理对紫花苜蓿抗寒性影响的代谢组学分析[J]. 草业学报, 2020, 29(1): 106-116.

XU Hong-yu, LI Xiang-lin. A metabolomics analysis of the effect of water deficit on the freezing tolerance of alfalfa (Medicago sativa)[J]. Acta Prataculturae Sinica, 2020, 29(1): 106-116.

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