甘肃马先蒿寄生对禾草内生真菌共生体共生关系的影响

doi:10.11686/cyxb2019242

草业学报 ›› 2020, Vol. 29 ›› Issue (2): 42-51.DOI: 10.11686/cyxb2019242

甘肃马先蒿寄生对禾草内生真菌共生体共生关系的影响

鲍根生^1,2,*, 宋梅玲^1,2, 王玉琴^1,2, 李春杰³

1.青海大学畜牧兽医科学院,青藏高原优良牧草种质资源研究重点实验室,青海西宁 810016;
2.青海大学省部共建三江源生态与高原农牧业国家重点实验室,青海西宁 810003;
3.兰州大学草地农业生态系统国家重点实验室,甘肃兰州 730020

收稿日期:2019-04-10 修回日期:2019-07-08 出版日期:2020-02-20 发布日期:2020-02-20
通讯作者: E-mail: baogensheng2008@hotmail.com
作者简介:鲍根生(1980-),男,青海乐都人,博士。E-mail: baogensheng2008@hotmail.com
基金资助:
国家自然科学基金项目(31660690, 31700098)和青海省“高端创新人才千人计划”拔尖人才培养计划项目资助

Effects of parasitism by a root hemiparasite on mutualistic relationship between host grasses and their Epichloë endophytes

BAO Gen-sheng^1,2,*, SONG Mei-ling^1,2, WANG Yu-qin^1,2, LI Chun-jie³

1.Key Laboratory of Qinghai-Tibet Plateau Forage Germplasm Research, Academy of Animal and Veterinary Medicine, Xining 810016, China;
2.State Key Laboratory Plateau Ecology and Agriculture, Qinghai University, Xining 810003, China;
3.Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Received:2019-04-10 Revised:2019-07-08 Online:2020-02-20 Published:2020-02-20
Contact: E-mail: baogensheng2008@hotmail.com

摘要/Abstract

摘要： 根寄生植物-甘肃马先蒿是高寒草地重度退化阶段主要滋生毒杂草种类,利用根部吸器与侵染内生真菌的禾草(紫花针茅和麦宾草)建立寄生关系。当禾草成为甘肃马先蒿和内生真菌共同寄主(宿主)时,有关内生真菌调控甘肃马先蒿对禾草根寄生危害的相关研究较少。以侵染Epichloë属内生真菌(E+)和未侵染内生真菌(E-)的紫花针茅和麦宾草为研究对象,观测甘肃马先蒿寄生对E+、E-紫花针茅和麦宾草株高、分蘖、根茎比、生物量变化和根部吸器数量及显微结构差异。结果表明:甘肃马先蒿寄生显著降低紫花针茅和麦宾草株高、分蘖和生物量,内生真菌侵染显著影响甘肃马先蒿根寄生处理禾草的生长特性。紫花针茅E+、E-植株被甘肃马先蒿寄生后,E+植株分蘖、生物量和根茎比明显高于E-植株,而E-植株根部功能性吸器数量明显高于E+植株; 麦宾草与甘肃马先蒿建立寄生关系后,E-植株株高、分蘖、生物量和根茎比明显高于E+植株,E+植株根部功能性吸器数量明显高于E-植株。由此可见,甘肃马先蒿与禾草建立寄生关系后,内生真菌与禾草间的共生关系处于互惠共生和相互拮抗的动态变化,草地群落其他植物与禾草间相互作用能调节禾草与内生真菌的共生关系。

关键词: 内生真菌, 紫花针茅, 根寄生植物, 互利共生, 拮抗, 甘肃马先蒿

Abstract: Pedidcularis kansuensis, a root hemiparasite plant, is widely distributed in the degraded grassland of Qinghai-Tibet Plateau. It can establish parasitic root associations with host grasses through haustoria. However, few studies examine the effect of Epichloë endophyte on host grass performance when the host grasses are common hosts of both P. kansuensis and Epichloë endophyte. To study this question, we evaluated the effects of endophyte infection and parasitism of P. kansuensis on plant height, tiller number, biomass, root∶shoot ratio and haustorial anatomy of two host grasses, Stipa purpurea and Elymus tangutorum. Plant height, tiller number, and biomass of the host plants decreased in the presence of P. kansuensis. However, the impact of Epichloë endophyte on host grass performance differed for the two host species studied. Tiller number, biomass and root:shoot ratio of E+ S. purpurea were significantly higher than for their E- counterparts, while the number functional haustoria (FHa) of E+ S. purpurea was lower than in E- plants. By contrast, plant height, tiller number, biomass and root:shoot ratio of E- E. tangutorum were higher than for the E+ counterparts, while the number of FHa of E+ E. tangutorum plants was more than for E- plants. Hence, these results suggest that interactions between the Epichloë endophyte and host grasses are species-dependent, ranging from mutualism to parasitism, and also display a plant genotype x endophyte strain interaction that can strongly affect the mutualistic interaction between the host grass and the Epichloë endophyte.

Key words: Epichloë endophyte;, Stipa purpurea, root hemiparasite, mutualism, antagonism, Pedicularis kansuensis

鲍根生, 宋梅玲, 王玉琴, 李春杰. 甘肃马先蒿寄生对禾草内生真菌共生体共生关系的影响[J]. 草业学报, 2020, 29(2): 42-51.

BAO Gen-sheng, SONG Mei-ling, WANG Yu-qin, LI Chun-jie. Effects of parasitism by a root hemiparasite on mutualistic relationship between host grasses and their Epichloë endophytes[J]. Acta Prataculturae Sinica, 2020, 29(2): 42-51.

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甘肃马先蒿寄生对禾草内生真菌共生体共生关系的影响

Effects of parasitism by a root hemiparasite on mutualistic relationship between host grasses and their Epichloë endophytes

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