欢迎访问《草业学报》官方网站,今天是 分享到:

草业学报 ›› 2020, Vol. 29 ›› Issue (6): 14-26.DOI: 10.11686/cyxb2019418

• 研究论文 • 上一篇    下一篇

甘草属杂交区物种形态特征的数值分类学研究

陈雯清1, 陆嘉惠1,2,*, 王倩倩1, 辛倩1, 徐影1   

  1. 1.石河子大学生命科学学院,新疆植物药资源利用教育部重点实验室,新疆 石河子 832003;
    2.石河子大学甘草研究所,新疆 石河子 832003
  • 收稿日期:2019-09-29 修回日期:2019-12-03 出版日期:2020-06-20 发布日期:2020-06-20
  • 通讯作者: * E-mail: jiahuil@shzu.edu.cn
  • 作者简介:陈雯清(1994-),女,河南信阳人,在读硕士。E-mail: 1607749254@qq.com
  • 基金资助:
    国家自然科学基金项目(31760046)资助

Numerical taxonomic study of morphological characteristics of species in Glycyrrhiza hybrid zones

CHEN Wen-qing1, LU Jia-hui1,2,*, WANG Qian-qian1, XIN Qian1, XU Ying1   

  1. 1. College of Life Sciences, Key Laboratory of Xinjiang Phytomedicine Resource Utilization, Ministry of Education, Shihezi University, Shihezi 832003, China;
    2. Institute of Licorce, Shihezi University, Shihezi 832003, China
  • Received:2019-09-29 Revised:2019-12-03 Online:2020-06-20 Published:2020-06-20

摘要: 本研究对来自新疆和甘肃9个甘草属杂交区物种的形态分类性状进行数值分类分析,以区别和鉴定不同杂交区的亲本种组成及杂交类型,为药用甘草种质资源利用和杂交物种形成研究奠定形态分类学基础。通过野外调查和标本采集,对形态学特征及数据进行记录和测量;数值化处理后,进行主成分分析,提取具有分类价值的主要特征性状;通过R聚类对29个性状进行分类;通过OTU散点和Q聚类对不同杂交区的亲本种及其杂交类型进行分类。结果表明:在前3个主成分中果荚膨胀度(LeI)、小叶数上限(ULF)、小叶数下限(LLF)、果荚被腺体毛(LeG)、复叶长(CLL)、每荚含种子数(LePS)、复叶轴长(CRL)、小叶叶缘(LM)、果穗长/果穗宽(EL/EW)、果穗长(EL)、小叶长/小叶宽(LL/LW)、小叶宽(LW)的绝对权重值均在0.7以上,这12个性状在甘草属植物的分类学研究中具有重要价值。29个性状被归为2类:A类主要为叶片和果穗相关的性状;B类主要为果荚和复叶相关的性状,两类性状间的相关系数皆为1,表明这些性状相伴发生,具有很高的相关性。344份材料被分为A类胀果甘草;B类胀果甘草和乌拉尔甘草的杂交类型;C类乌拉尔甘草;D类光果甘草;E类胀果甘草和光果甘草的杂交类型。A类胀果甘草包括新疆阿拉尔、库尔勒和甘肃瓜州、民勤的胀果甘草,聚为一支,表明胀果甘草的不同居群遗传分化较小,形态特征不存在地域分布差异;C类甘肃民勤、张掖和新疆库尔勒、焉耆、博湖的乌拉尔甘草,分别被聚为两支,表明乌拉尔甘草出现种内遗传分化,甘肃和新疆不同分布区存在明显地域差异;D类光果甘草包括新疆阿拉尔的密腺甘草和光果甘草,支持密腺甘草为光果甘草的变种。9个自然杂交区类型分为3种:新疆阿拉尔、吐鲁番为胀果甘草与光果甘草的种间杂交区;新疆焉耆、库尔勒和甘肃瓜州为胀果甘草与乌拉尔甘草的种间杂交区;鄯善为胀果甘草、光果甘草及乌拉尔甘草的混合杂交区。所有杂交区的杂交类型均具有不同的种间镶嵌式形态特征。

关键词: 甘草属, 杂交区, 形态特征, 数值分类

Abstract: In this study, the morphological characters of Glycyrrhiza specimens from nine regions of Xinjiang and Gansu Provinces where natural hybrids occur were classified by numerical taxonomic study to identify the parent species and hybridization type in different geographic areas where hybrids occur. Following field investigation and specimen collection, data on morphological traits were measured and recorded. After the numerical data collection, principal component analysis was carried out to identify the most important traits for classification purposes, and 29 traits were evaluated by R-cluster. The parental species and their hybrids in the different areas of hybrid occurrence were classified by operational taxonomic unit scatter and Q clustering. Principal component analysis result shows that Among the first three principal components, degree of legume inflation, upper limit of foliolate , lower limit of foliolate, presence of legume glandular hair, compound leaf length, no. of seeds per pod, compound rachis length, leaflet margin morphology, infructescence length, infructescence length:width ratio, leaflet length:leaflet width ratio, and leaflet width all are above 0.7, meaning that these twelve characters play an important role in taxonomic study on Glycyrrhiza species. The 29 traits could be classified into 2 categories: the first, mainly comprising leaflet and inflorescence traits, and the second, mainly comprising legume and compound leaf traits. The correlation coefficient between scores of the two categories of traits was 1, indicating that these trait groupings occur in conjunction and have high relevance as taxonomic indicators. Glycyrrhiza accessions (n=344) were classified into groups as follows: A, Glycyrrhiza inflata; B, The hybrid of G. inflata and Glycyrrhiza uralensis; C, G. uralensis; D, Glycyrrhiza glabra; E, the hybrid of G. inflata and G. glabra. A variation of G. inflata includes plants from Alaer and Korla in Xinjiang, and Guazhou and Minqin in Gansu Provinces, which are gathered into one branch, as these populations of G. inflata are less genetically differentiated. Plants of group C from Minqin, Zhangye in Gansu and from Korla, Yanqi, and Bohu in Xinjiang, were divided into two branches, as intravarietal genetic differentiation had occurred. Group D representing G. glabra. includes G. glabra and Glycyrrhiza glabra var. glandulosa from Alaer, Xinjiang, as G. glabra var. glandulosa is a variety of G. glabra. Nine geographic areas where natural interspecific hybrids occur could be divided into three categories: Alar and Turpan in Xinjiang Province are interspecific hybridization areas of G. inflata and G. glabra; Yanqi and Korla in Xinjiang and Guazhou in Gansu are interspecific hybridization areas of G. inflata and G. uralensis; Shanshan in Xinjiang is a mixed hybridization area of G. inflata, G. glabra and G. uralensis. All hybrid specimens of Glycyrrhiza examined, have distinctive and different inter-species combinations of morphological features. This study laid the foundation of morphological taxonomy for the utilization of medicinal Glycyrrhiza germplasm resources and the formation ofhybrid species.

Key words: Glycyrrhiza, hybrid zones, morphological features, numerical taxonomy