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草业学报 ›› 2021, Vol. 30 ›› Issue (4): 180-190.DOI: 10.11686/cyxb2020184

• 研究论文 • 上一篇    下一篇

饲养水平对阿勒泰羊胃肠道发育、瘤胃发酵参数及瘤胃微生物区系的影响

李宏1,2(), 宋淑珍1(), 高良霜2, 郎侠1, 刘立山1, 宫旭胤1, 魏玉兵4, 吴建平1,3()   

  1. 1.甘肃省农业科学院畜草与绿色农业研究所,甘肃省牛羊种质与秸秆饲料化重点实验室,甘肃 兰州 730070
    2.甘肃农业大学动物科学技术学院,甘肃 兰州 730070
    3.西北师范大学新农村发展研究院,甘肃 兰州 730070
    4.平山湖蒙古族乡畜牧兽医站,甘肃 张掖 734000
  • 收稿日期:2020-04-21 修回日期:2020-07-02 出版日期:2021-04-20 发布日期:2021-03-16
  • 通讯作者: 吴建平
  • 作者简介:Corresponding author. E-mail: wujp@gsagr.ac.cn
    李宏(1993-),男,藏族,甘肃天祝人,在读硕士。E-mail: mattlh520@163.com
    宋淑珍(1980-),女,甘肃通渭人,副研究员,博士。E-mail: songshuzhen@gsagr.ac.cn第一联系人:共同第一作者These authors contributed equally to these work.
  • 基金资助:
    国家自然科学基金项目(31460592)

Effects of feeding level on the gastrointestinal development, rumen fermentation and rumen microbiota in Altay sheep

Hong LI1,2(), Shu-zhen SONG1(), Liang-shuang GAO2, Xia LANG1, Li-shan LIU1, Xu-yin GONG1, Yu-bing WEI4, Jian-ping WU1,3()   

  1. 1.Institute of Livestock and Green Agriculture Research,Gansu Academy of Agricultural Sciences,Key Laboratory of Cattle and Sheep Germplasm and Straw Feed in Gansu Province,Lanzhou 730070,China
    2.College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China
    3.College of New Rural Development Research,Northwest Normal University,Lanzhou 730070,China
    4.Animal Husbandry and Veterinary Station of Pingshanhu,Zhangye 734000,China
  • Received:2020-04-21 Revised:2020-07-02 Online:2021-04-20 Published:2021-03-16
  • Contact: Jian-ping WU

摘要:

为研究饲养水平对阿勒泰羊胃肠道发育、瘤胃发酵参数、消化酶活性及瘤胃微生物区系的影响,选取月龄相近(3~3.5月龄)、体重均一(19.16±0.54) kg、臀型一致、健康状况良好的阿勒泰母羔羊30只,随机分为3组,每组10只,自由采食60 d后,参照NRC(2007)饲养标准中维持能量(Em)需要,3个组分别按1.5Em(0.75 MJ·kg-1 W0.75)、1.0Em(0.50 MJ·kg-1 W0.75)和0.5Em(0.25 MJ·kg-1 W0.75)饲喂30 d。结果表明:1)随饲养水平的降低,羔羊全胃、瘤胃、小肠的重量逐渐降低,且1.5Em组全胃、瘤胃、小肠重量和瘤胃指数显著高于1.0Em和0.5Em组(P<0.05),1.5Em和1.0Em组小肠长度和小肠指数显著高于0.5Em组(P<0.05),但1.5Em和1.0Em组无显著差异(P>0.05);2)随饲养水平的降低,绵羊瘤胃液pH先升高后降低,1.5Em组pH显著低于0.5Em和1.0Em组(P<0.05),氨态氮(NH3-N)含量逐渐降低但3组之间的差异不显著(P>0.05),总挥发性脂肪酸(TVFA)以及乙酸、丙酸、丁酸、戊酸、异丁酸、异戊酸含量1.0Em组最低,且1.0Em组TVFA、乙酸、丙酸含量显著低于1.5Em和0.5Em组(P<0.05),丁酸、戊酸和乙酸/丙酸1.0Em和0.5Em组显著低于1.5Em组(P<0.05);3)随饲养水平的降低,瘤胃液消化酶活性降低,脂肪酶、蛋白酶活性1.5Em组显著高于1.0Em和0.5Em组(P<0.05),1.0Em和0.5Em组之间差异不显著(P>0.05),α-淀粉酶3组之间差异显著(P<0.05);4)瘤胃内容物中真菌、原虫的相对含量1.5Em组显著高于1.0Em和0.5Em组(P<0.05),但1.0Em和0.5Em组之间差异不显著(P>0.05),产琥珀酸丝状杆菌、白色瘤胃球菌1.0Em组最低,显著低于1.5Em和0.5Em组(P<0.05),黄色瘤胃球菌3组之间无显著变化(P>0.05)。由此可见,饲养水平对阿勒泰羊胃肠道发育、瘤胃发酵参数、消化酶活性及瘤胃微生物区系都具有显著影响。

关键词: 饲养水平, 阿勒泰羊, 胃肠道发育, 发酵参数, 消化酶, 瘤胃微生物区系

Abstract:

The purpose of this experiment was to study the effects of relative feeding level on rate of bodyweight gain, carcass dressing out performance, meat quality and organ development in Altay sheep. Thirty 3.0 to 3.5 month old Altay female lambs with a similar body weight of (19.16±10.54) kg and uniform tail size were randomly divided into 3 groups with 10 lambs per group. After 60 daysof adlibitumfeeding, three differential feeding levels were imposed for a further 30 days. The differential feeding levels were calculated based on feeding standards published by the National Research Council, USA, in 2007, defining maintenance energy (Em) requirements and lambs in each group were fed diets supplying 1.5× (1.5Em), 1.0× (1.0Em) or 0.5× (0.5Em) the calculated Em (0.75, 0.50 and 0.25 MJ metabolizable energy per kg0.75 body weight, respectively). It was found that: 1) The weights of the whole stomach, rumen, and small intestine and a rumen size index decreased with decrease in feeding level, and the weights of the whole stomach, rumen and small intestine fed at 1.5Em were significantly higher (P<0.05) than in groups fed 1.0Em and 0.5Em, while the length of small intestine and a small intestine size index in groups fed 1.5Em and 1.0Em were significantly increased (P<0.05) compared with the group fed 0.5Em. And there was no significant difference between the 1.5Em and the 1.0Em groups (P>0.05). 2) The pH of rumen fluid was highest in the 1.0Em group and rumen pH for the group fed 1.5Em was significantly lower (P<0.05) than both the 1.0Em and 0.5Em groups. NH3-N content decreased gradually with decrease feeding level, but the differences between the three groups was not statistically significant (P>0.05). Total volatile fatty acids (TVFA), acetic acid, propionic acid, butyric acid, valeric acid, isobutyric acid and isovaleric acid concentrations in rumen fluid of lambs fed 1.0Em were the lowest, and rumen TVFA, acetic acid and propionic acid levels of lambs fed 1.0Em were significantly lower (P<0.05) than in lambs fed 1.5Em and 0.5Em. The length of the small intestine and the small intestine size index of lambs fed 1.5Em and 1.0Em were increased significantly (P<0.05) compared to lambs fed 0.5Em. Rumen butyric acid and valproic acid concentrations and acetic∶propionic acid in groups fed 1.0Em and 0.5Em groups were significantly lower (P<0.05) than in the group fed 1.5Em. 3) The activities of digestive enzymes in rumen fluid decreased with the decrease of feeding level; the activities of lipase and protease in the group fed 1.5Em were significantly higher (P<0.05) than in the groups fed 1.0Em and 0.5Em, and there was no significant difference (P>0.05) between the groups fed 1.0Em and 0.5Em. Alpha-amylase activity differed significantly (P<0.05) among three groups. 4) The relative abundance of fungi and protozoa in rumen fluid of lambs fed 1.5Em was significantly higher (P<0.05) than in lambs fed 1.0Em and 0.5Em, and the differences between 1.0Em and 0.5Em groups were not significant (P>0.05). Counts of Fibrobacter succinogenes and Ruminococcus albus were the lowest in lambs fed 1.0Em, and were significantly lower (P<0.05) than counts in the 1.5Em and 0.5Em groups. Ruminococcus flavefaciens counts did not differ significantly among the three groups (P>0.05). These results show that feeding level has a significant effect on the gastrointestinal development, rumen fermentation parameters, activities of digestive enzymes and rumen microbiota in Altay sheep.

Key words: feeding level, Altay sheep, gastrointestinal development, fermentation parameters, digestive enzyme, microbial flora