草业学报 ›› 2021, Vol. 30 ›› Issue (4): 180-190.DOI: 10.11686/cyxb2020184
李宏1,2(), 宋淑珍1(), 高良霜2, 郎侠1, 刘立山1, 宫旭胤1, 魏玉兵4, 吴建平1,3()
收稿日期:
2020-04-21
修回日期:
2020-07-02
出版日期:
2021-04-20
发布日期:
2021-03-16
通讯作者:
吴建平
作者简介:
Corresponding author. E-mail: wujp@gsagr.ac.cn基金资助:
Hong LI1,2(), Shu-zhen SONG1(), Liang-shuang GAO2, Xia LANG1, Li-shan LIU1, Xu-yin GONG1, Yu-bing WEI4, Jian-ping WU1,3()
Received:
2020-04-21
Revised:
2020-07-02
Online:
2021-04-20
Published:
2021-03-16
Contact:
Jian-ping WU
摘要:
为研究饲养水平对阿勒泰羊胃肠道发育、瘤胃发酵参数、消化酶活性及瘤胃微生物区系的影响,选取月龄相近(3~3.5月龄)、体重均一(19.16±0.54) kg、臀型一致、健康状况良好的阿勒泰母羔羊30只,随机分为3组,每组10只,自由采食60 d后,参照NRC(2007)饲养标准中维持能量(Em)需要,3个组分别按1.5Em(0.75 MJ·kg-1 W0.75)、1.0Em(0.50 MJ·kg-1 W0.75)和0.5Em(0.25 MJ·kg-1 W0.75)饲喂30 d。结果表明:1)随饲养水平的降低,羔羊全胃、瘤胃、小肠的重量逐渐降低,且1.5Em组全胃、瘤胃、小肠重量和瘤胃指数显著高于1.0Em和0.5Em组(P<0.05),1.5Em和1.0Em组小肠长度和小肠指数显著高于0.5Em组(P<0.05),但1.5Em和1.0Em组无显著差异(P>0.05);2)随饲养水平的降低,绵羊瘤胃液pH先升高后降低,1.5Em组pH显著低于0.5Em和1.0Em组(P<0.05),氨态氮(NH3-N)含量逐渐降低但3组之间的差异不显著(P>0.05),总挥发性脂肪酸(TVFA)以及乙酸、丙酸、丁酸、戊酸、异丁酸、异戊酸含量1.0Em组最低,且1.0Em组TVFA、乙酸、丙酸含量显著低于1.5Em和0.5Em组(P<0.05),丁酸、戊酸和乙酸/丙酸1.0Em和0.5Em组显著低于1.5Em组(P<0.05);3)随饲养水平的降低,瘤胃液消化酶活性降低,脂肪酶、蛋白酶活性1.5Em组显著高于1.0Em和0.5Em组(P<0.05),1.0Em和0.5Em组之间差异不显著(P>0.05),α-淀粉酶3组之间差异显著(P<0.05);4)瘤胃内容物中真菌、原虫的相对含量1.5Em组显著高于1.0Em和0.5Em组(P<0.05),但1.0Em和0.5Em组之间差异不显著(P>0.05),产琥珀酸丝状杆菌、白色瘤胃球菌1.0Em组最低,显著低于1.5Em和0.5Em组(P<0.05),黄色瘤胃球菌3组之间无显著变化(P>0.05)。由此可见,饲养水平对阿勒泰羊胃肠道发育、瘤胃发酵参数、消化酶活性及瘤胃微生物区系都具有显著影响。
李宏, 宋淑珍, 高良霜, 郎侠, 刘立山, 宫旭胤, 魏玉兵, 吴建平. 饲养水平对阿勒泰羊胃肠道发育、瘤胃发酵参数及瘤胃微生物区系的影响[J]. 草业学报, 2021, 30(4): 180-190.
Hong LI, Shu-zhen SONG, Liang-shuang GAO, Xia LANG, Li-shan LIU, Xu-yin GONG, Yu-bing WEI, Jian-ping WU. Effects of feeding level on the gastrointestinal development, rumen fermentation and rumen microbiota in Altay sheep[J]. Acta Prataculturae Sinica, 2021, 30(4): 180-190.
成分 Ingredient | 含量 Content (%) | 营养Nutrient 2 | 水平Level |
---|---|---|---|
苜蓿干草Alfalfa hay | 25.40 | 消化能Metabolizable energy (DE, MJ·kg-1) | 10.66 |
玉米秆Corn straw | 19.40 | 代谢能Digestible energy (ME, MJ·kg-1) | 8.67 |
小麦秆Wheat straw | 5.00 | 粗蛋白质Crude protein (%) | 12.51 |
玉米Corn | 30.00 | 粗脂肪Ether extract (%) | 2.13 |
大豆粕Soybean meal | 10.00 | 钙Calcium (%) | 0.65 |
麸皮Wheat bran | 5.00 | 总磷Total phosphorus (%) | 0.45 |
菜籽粕Rapeseed meal | 4.00 | 中性洗涤纤维Neutral detergent fibre (%) | 55.30 |
磷酸氢钙Calcium hydrogen phosphate | 0.20 | 酸性洗涤纤维Acid detergent fibre (%) | 32.22 |
碳酸钙Calcium carbonate | 0.35 | ||
氯化钠Sodium chloride | 0.50 | ||
预混料Premixture1 | 0.15 | ||
合计Total | 100.00 |
表1 饲粮组成及营养水平
Table 1 Composition and nutrient levels of experimental diets (Air-dry basis)
成分 Ingredient | 含量 Content (%) | 营养Nutrient 2 | 水平Level |
---|---|---|---|
苜蓿干草Alfalfa hay | 25.40 | 消化能Metabolizable energy (DE, MJ·kg-1) | 10.66 |
玉米秆Corn straw | 19.40 | 代谢能Digestible energy (ME, MJ·kg-1) | 8.67 |
小麦秆Wheat straw | 5.00 | 粗蛋白质Crude protein (%) | 12.51 |
玉米Corn | 30.00 | 粗脂肪Ether extract (%) | 2.13 |
大豆粕Soybean meal | 10.00 | 钙Calcium (%) | 0.65 |
麸皮Wheat bran | 5.00 | 总磷Total phosphorus (%) | 0.45 |
菜籽粕Rapeseed meal | 4.00 | 中性洗涤纤维Neutral detergent fibre (%) | 55.30 |
磷酸氢钙Calcium hydrogen phosphate | 0.20 | 酸性洗涤纤维Acid detergent fibre (%) | 32.22 |
碳酸钙Calcium carbonate | 0.35 | ||
氯化钠Sodium chloride | 0.50 | ||
预混料Premixture1 | 0.15 | ||
合计Total | 100.00 |
项目Item | 引物序列 Primer sequences (5'-3') | 退火温度Annealing temperature (℃) | 扩增片段Amplification size (bp) |
---|---|---|---|
细菌Bacterial | F: CCTACGGGAGGCAGCAG R: ATTACCGCGGCTGCTGG | 60 | 181[ |
原虫Protozoa | F: TGACTCAACACGGGGAAACT R: TCCACCAACTAAGAACGGCC | 60 | 109[ |
真菌Fungi | F: TGACTCAACACGGGGAAACT R: CCAACTAAGAACGGCCATGC | 60 | 105[ |
白色瘤胃球菌 Ruminococcu ablus | F: ATGCCGCGGTGAATACGTT R: TTCGACTGCTTCCTCCTTGC | 64 | 139[ |
产琥珀酸丝状杆菌 Fibrobacter succinogenes | F: GATGAGCTTGCGTCCGATT R: ATTCCCTACTGCTGCCTCC | 60 | 107[ |
黄色瘤胃球菌 Ruminococcus flavefaciens | F: ATTGTCCCAGTTCAGATTGC R: GGCGTCCTCATTGCTGTTAG | 60 | 131[ |
表2 瘤胃微生物引物序列
Table 2 The primer sequence of rumen microorganisms
项目Item | 引物序列 Primer sequences (5'-3') | 退火温度Annealing temperature (℃) | 扩增片段Amplification size (bp) |
---|---|---|---|
细菌Bacterial | F: CCTACGGGAGGCAGCAG R: ATTACCGCGGCTGCTGG | 60 | 181[ |
原虫Protozoa | F: TGACTCAACACGGGGAAACT R: TCCACCAACTAAGAACGGCC | 60 | 109[ |
真菌Fungi | F: TGACTCAACACGGGGAAACT R: CCAACTAAGAACGGCCATGC | 60 | 105[ |
白色瘤胃球菌 Ruminococcu ablus | F: ATGCCGCGGTGAATACGTT R: TTCGACTGCTTCCTCCTTGC | 64 | 139[ |
产琥珀酸丝状杆菌 Fibrobacter succinogenes | F: GATGAGCTTGCGTCCGATT R: ATTCCCTACTGCTGCCTCC | 60 | 107[ |
黄色瘤胃球菌 Ruminococcus flavefaciens | F: ATTGTCCCAGTTCAGATTGC R: GGCGTCCTCATTGCTGTTAG | 60 | 131[ |
项目Items | 1.5Em | 1.0Em | 0.5Em |
---|---|---|---|
全胃重 Whole stomach weight (kg) | 0.73±0.14a | 0.53±0.10b | 0.47±0.04b |
瘤胃重Rumen weight (kg) | 0.57±0.09a | 0.41±0.08b | 0.38±0.03b |
小肠重Small intestine weight (kg) | 0.61±0.03a | 0.55±0.06b | 0.43±0.03c |
小肠长Small bowel length (m) | 30.73±1.88a | 29.68±4.21a | 25.02±2.31b |
瘤胃占宰前活重比例Rumen ratio of live weight before slaughter (%) | 1.73±0.15a | 1.20±0.15b | 1.25±0.16b |
小肠占宰前活重比例Proportion of small intestine living weight before slaughter (%) | 1.88±0.24a | 1.64±0.31a | 1.43±0.21b |
表3 饲养水平对绵羊胃肠道发育的影响
Table 3 Effect of feeding level on the development of the gastrointestinal tract in sheep
项目Items | 1.5Em | 1.0Em | 0.5Em |
---|---|---|---|
全胃重 Whole stomach weight (kg) | 0.73±0.14a | 0.53±0.10b | 0.47±0.04b |
瘤胃重Rumen weight (kg) | 0.57±0.09a | 0.41±0.08b | 0.38±0.03b |
小肠重Small intestine weight (kg) | 0.61±0.03a | 0.55±0.06b | 0.43±0.03c |
小肠长Small bowel length (m) | 30.73±1.88a | 29.68±4.21a | 25.02±2.31b |
瘤胃占宰前活重比例Rumen ratio of live weight before slaughter (%) | 1.73±0.15a | 1.20±0.15b | 1.25±0.16b |
小肠占宰前活重比例Proportion of small intestine living weight before slaughter (%) | 1.88±0.24a | 1.64±0.31a | 1.43±0.21b |
项目Items | 1.5Em | 1.0Em | 0.5Em |
---|---|---|---|
pH | 6.69±0.17b | 7.31±0.10a | 7.17±0.09a |
氨态氮NH3-N (mg·dL-1) | 7.61±2.86a | 6.11±0.70a | 5.88±1.04a |
总挥发性脂肪酸Total volatile fatty acid (mmol·L-1) | 39.79±9.07a | 25.30±1.02b | 36.54±3.28a |
乙酸Acetic acid (mmol·L-1) | 21.55±5.93a | 12.41±0.91b | 19.19±2.85a |
丙酸Propionic acid (mmol·L-1) | 10.86±1.72a | 8.20±0.15b | 10.89±0.75a |
丁酸Butyrate (mmol·L-1) | 4.61±1.28a | 2.77±0.19b | 3.45±0.41b |
戊酸Valerate (mmol·L-1) | 0.83±0.10a | 0.68±0.02b | 0.81±0.12ab |
异丁酸Isobutyric acid (mmol·L-1) | 0.83±0.08b | 0.80±0.10b | 0.95±0.06a |
异戊酸Isovalerate (mmol·L-1) | 1.10±0.12ab | 1.03±0.17b | 1.24±0.08a |
乙酸/丙酸Acetate/propionate | 1.95±0.29a | 1.51±0.10b | 1.77±0.27ab |
表4 饲养水平对绵羊瘤胃发酵参数的影响
Table 4 Effect of feeding level on rumen fermentation in sheep
项目Items | 1.5Em | 1.0Em | 0.5Em |
---|---|---|---|
pH | 6.69±0.17b | 7.31±0.10a | 7.17±0.09a |
氨态氮NH3-N (mg·dL-1) | 7.61±2.86a | 6.11±0.70a | 5.88±1.04a |
总挥发性脂肪酸Total volatile fatty acid (mmol·L-1) | 39.79±9.07a | 25.30±1.02b | 36.54±3.28a |
乙酸Acetic acid (mmol·L-1) | 21.55±5.93a | 12.41±0.91b | 19.19±2.85a |
丙酸Propionic acid (mmol·L-1) | 10.86±1.72a | 8.20±0.15b | 10.89±0.75a |
丁酸Butyrate (mmol·L-1) | 4.61±1.28a | 2.77±0.19b | 3.45±0.41b |
戊酸Valerate (mmol·L-1) | 0.83±0.10a | 0.68±0.02b | 0.81±0.12ab |
异丁酸Isobutyric acid (mmol·L-1) | 0.83±0.08b | 0.80±0.10b | 0.95±0.06a |
异戊酸Isovalerate (mmol·L-1) | 1.10±0.12ab | 1.03±0.17b | 1.24±0.08a |
乙酸/丙酸Acetate/propionate | 1.95±0.29a | 1.51±0.10b | 1.77±0.27ab |
项目 Item | 1.5Em | 1.0Em | 0.5Em |
---|---|---|---|
α-淀粉酶 α-amylase | 50.88±5.45a | 25.18±5.81c | 33.18±6.28b |
脂肪酶 Lipase | 12.21±7.15a | 6.92±1.95b | 5.86±0.91b |
胃蛋白酶 Pepsase | 116.56±21.90a | 71.42±11.43b | 52.33±21.78b |
表5 饲养水平对绵羊瘤胃消化酶活性的影响
Table 5 Effect of feeding level on the activity of the digestive enzymes in the rumen
项目 Item | 1.5Em | 1.0Em | 0.5Em |
---|---|---|---|
α-淀粉酶 α-amylase | 50.88±5.45a | 25.18±5.81c | 33.18±6.28b |
脂肪酶 Lipase | 12.21±7.15a | 6.92±1.95b | 5.86±0.91b |
胃蛋白酶 Pepsase | 116.56±21.90a | 71.42±11.43b | 52.33±21.78b |
项目 Item | 1.5Em | 1.0Em | 0.5Em |
---|---|---|---|
真菌 Fungi | 3.98±1.79a | 1.34±0.17b | 1.21±0.12b |
原虫 Protozoa | 1.97±0.76a | 0.40±0.19b | 0.63±0.08b |
产琥珀酸丝状杆菌 F. succinogenes | 1.15±0.29a | 0.60±0.14b | 1.25±0.29a |
白色瘤胃球菌 R. ablus | 1.60±0.43a | 1.01±0.12b | 1.63±0.32a |
黄色瘤胃球菌 R. flavefaciens | 1.16±0.75a | 2.54±1.42a | 1.79±0.50a |
表6 饲养水平对阿勒泰羊瘤胃微生物区系的影响
Table 6 Effect of feeding level on rumen microbial flora in Altay sheep
项目 Item | 1.5Em | 1.0Em | 0.5Em |
---|---|---|---|
真菌 Fungi | 3.98±1.79a | 1.34±0.17b | 1.21±0.12b |
原虫 Protozoa | 1.97±0.76a | 0.40±0.19b | 0.63±0.08b |
产琥珀酸丝状杆菌 F. succinogenes | 1.15±0.29a | 0.60±0.14b | 1.25±0.29a |
白色瘤胃球菌 R. ablus | 1.60±0.43a | 1.01±0.12b | 1.63±0.32a |
黄色瘤胃球菌 R. flavefaciens | 1.16±0.75a | 2.54±1.42a | 1.79±0.50a |
1 | Li Y, Gao M, Hu H L, et al. Absorption mechanism of volatile fatty acids in rumen of ruminants. Chinese Journal of Animal Nutrition, 2018, 30(6): 2070-2078. |
李洋, 高民, 胡红莲, 等. 反刍动物瘤胃挥发性脂肪酸的吸收机制. 动物营养学报, 2018, 30(6): 2070-2078. | |
2 | Liu K L, Wang J Q, Bu P D. Advances in ruminant nutrition research, 2008-2009. Microbial diversity and function in rumen. Animal Husbandry and Veterinary Medicine, 2010, 37(2): 5-14. |
刘开朗, 王加启, 卜登攀. 2008-2009年反刍动物营养研究进展.瘤胃微生物多样性与功能. 中国畜牧兽医, 2010, 37(2): 5-14. | |
3 | Zhang J K, Li L R, Wu W X, et al. Combination effect of straw supplementary alfalfa on rumen fermentation of goats. Pratacultural Science, 2014, 31(2): 313-320. |
张吉鹍, 李龙瑞, 吴文旋, 等. 稻草补饲苜蓿对山羊瘤胃发酵的组合效应. 草业科学, 2014, 31(2): 313-320. | |
4 | Qi H W, Zhong W G, Wang Y T, et al. Changes and influencing factors of microbacteriosis in cow tumor and stomach. Journal of China of Agricultural University, 2018, 23(9): 75-80. |
祁宏伟, 仲伟光, 王玉婷, 等. 牛瘤胃微生物菌群变化及其影响因素. 中国农业大学学报, 2018, 23(9): 75-80. | |
5 | Jiang S Z, Yang Z B, Yang W R, et al. Diets of differentially processed wheat alter ruminal fermentation parameters and microbial populations in beef cattle. Journal of Animal Science, 2015, 98(11): 5378-5385. |
6 | Wang Y Q, Zhao Q M, Shen Y, et al. Effects of rice bran and corn germ meal on rumen fermentation and microbial flora in lactating cows. Journal of Yangzhou University (Agriculture and Life Sciences Edition), 2019, 40(3): 65-71. |
王玉强, 赵倩明, 沈宇, 等. 日粮中添加米糠和玉米胚芽粕对泌乳奶牛瘤胃发酵及微生物菌群的影响. 扬州大学学报(农业与生命科学版), 2019, 40(3): 65-71. | |
7 | Ceconi I, Ruiz-Moreno M J, Dilorenzo N, et al. Effect of urea inclusion in diets containing corn dried distillers grains on feedlot cattle performance, carcass characteristics, ruminal fermentation, total tract digestibility, and purine derivatives-to-creatinine index. Journal of Animal Science, 2015, 93(1): 357-369. |
8 | Qi M L, Diao Q Y, Zhang N F. Progress of rumen development and its influencing factors in lambs. Chinese Journal of Animal Husbandry, 2015, 51(9): 77-81. |
祁敏丽, 刁其玉, 张乃锋. 羔羊瘤胃发育及其影响因素研究进展. 中国畜牧杂志, 2015, 51(9): 77-81. | |
9 | Liu B S, Wang W J, Sun X, et al. Effects of dietary fiber sources on growth performance, intestinal development and digestive enzyme activity of piglets. Acta Prataculturae Sinica, 2018, 27(9): 175-182. |
刘伯帅, 王文静, 孙骁, 等. 饲粮纤维源对仔猪生长性能、肠道发育及其消化酶活性的影响. 草业学报, 2018, 27(9): 175-182. | |
10 | Wang H R. Analysis of the mechanism of gastric acid poisoning in ruminant tumor and its nutritional control measures. Chinese Journal of Animal Nutrition, 2014, 26(10): 3140-3148. |
王洪荣. 反刍动物瘤胃酸中毒机制解析及其营养调控措施. 动物营养学报, 2014, 26(10): 3140-3148. | |
11 | Zhao W L. A study on the relationship between adipose tissue differentially expressed gene RETN, CAV1, PLA2G16 and lipid metabolism in Altay sheep tail. Shihezi: Shihezi University, 2015. |
赵伟利. 脂肪组织差异表达基因RETN、CAV1、PLA2G16与阿勒泰羊尾脂沉积代谢关系的研究. 石河子: 石河子大学, 2015. | |
12 | Wang Y W. Studies on polymorphism and expression of fat deposition related genes in Altay sheep. Beijing: Chinese Academy of Agricultural Sciences, 2014. |
王耀武. 阿勒泰羊脂肪沉积相关基因的多态性与表达研究. 北京: 中国农业科学院, 2014. | |
13 | Zhang M. Genetic analysis of quality traits and KRT36 genes in Altay wool. Urumqi: Xinjiang Agricultural University, 2016. |
张敏. 阿勒泰羊毛绒品质性状及KRT36基因遗传效应分析. 乌鲁木齐: 新疆农业大学, 2016. | |
14 | Xu X L. Genetic diversity analysis of Altai sheep, Sunita sheep and Urank sheep based on ecological morphological characteristics and structural genome. Yangzhou: Yangzhou University, 2013. |
徐晓莉. 阿勒泰羊、苏尼特羊和乌冉克羊基于生态形态特征和结构基因座的遗传多样性分析. 扬州: 扬州大学, 2013. | |
15 | Xiong B H, Luo Q Y, Zhao F, et al. Development of a table of feed ingredients and nutritional values in China (26th edition, 2015). Feed Wide Angle, 2015(23): 21-31. |
熊本海, 罗清尧, 赵峰, 等. 中国饲料成分及营养价值表(2015年第26版)制订说明. 饲料广角, 2015(23): 21-31. | |
16 | Feng Z C, Gao M. Improvement of the method for determination of ammonia nitrogen content in rumen liquid by colorimetry. Animal Husbandry and Feed Science, 2010, 31(Z1): 37. |
冯宗慈, 高民. 通过比色测定瘤胃液氨氮含量方法的改进. 畜牧与饲料科学, 2010, 31(Z1): 37. | |
17 | Muyzer G, De Waal E C, Uitterlinden A G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16SrRNA. Applied and Environmental Microbiology, 1993, 59(3): 695-700. |
18 | Zhou R, Zhao S G, Liu L S, et al. Effects of oat hay content on rumen fluid pH and microbiota in sheep. Chinese Journal of Animal Nutrition, 2016, 28(5): 1589-1597. |
周瑞, 赵生国, 刘立山, 等. 饲粮中燕麦干草含量对绵羊瘤胃液pH 及微生物区系的影响. 动物营养学报, 2016, 28(5): 1589-1597. | |
19 | Wang H R. Effects of different dietary concentration ratio and nitrogen source on the degradation of microbacteria and fibre substances in sheep rumen. Hohhot: Inner Mongolia Agricultural University, 2006. |
王海荣. 不同日粮精粗比及氮源对绵羊瘤胃纤维降解菌群和纤维物质降解的影响. 呼和浩特: 内蒙古农业大学, 2006. | |
20 | Yang C B, Yin Y L, Huang R L, et al. Principle and method of real-time quantitative RT-PCR. Journal of Immunology, 2003, 19(3): 145-150. |
阳成波, 印遇龙, 黄瑞林, 等. 实时定量RT-PCR的原理及方法. 免疫学杂志, 2003, 19(3): 145-150. | |
21 | Qi M L. Effects of dietary energy and protein level on growth performance and gastrointestinal development of lambs. Beijing: Chinese Academy of Agricultural Sciences, 2016. |
祁敏丽. 日粮能量和蛋白质水平对羔羊生长性能和胃肠道发育的影响. 北京: 中国农业科学院, 2016. | |
22 | Qi M L, Ma T W, Diao Q Y, et al. Effects of dietary nutrition restriction on growth, slaughter performance and organ development of sheep lambs in weaned lake. Journal of Animal Husbandry and Veterinary Medicine, 2016, 47(8): 1601-1609. |
祁敏丽, 马铁伟, 刁其玉, 等. 饲粮营养限制对断奶湖羊羔羊生长、屠宰性能以及器官发育的影响. 畜牧兽医学报, 2016, 47(8): 1601-1609. | |
23 | Tian F, Jin H, Xue S Y, et al. Effects of different nutrient levels on production performance and visceral development of bamee sheep. Breeding and Feed, 2017(2): 38-41. |
田丰, 金海, 薛树媛, 等. 不同营养水平日粮对巴美肉羊生产性能和内脏发育的影响. 养殖与饲料, 2017(2): 38-41. | |
24 | Qi M L, Chai J M, Wang B, et al. Effects of dietary nutrition restriction on growth performance and visceral organ development of early weaned lamb. Chinese Journal of Animal Nutrition, 2016, 28(2): 444-454. |
祁敏丽, 柴建民, 王波, 等. 饲粮营养限制对早期断奶湖羊羔羊生长性能以及内脏器官发育的影响. 动物营养学报, 2016, 28(2): 444-454. | |
25 | Lv X K. Effects of different diets on rumen development of 20-60 day old goat. Beijing: Chinese Academy of Agricultural Sciences, 2019. |
吕小康. 不同饲粮对20~60日龄山羊羔羊瘤胃发育的影响. 北京: 中国农业科学院, 2019. | |
26 | Diao Q Y. Key technologies for healthy sheep raising. Beijing: Chemical Industry Press, 2014. |
刁其玉. 图说健康养羊关键技术. 北京: 化学工业出版社, 2014. | |
27 | Xu G S, Diao Q Y, Deng K D, et al. Effects of limited feeding on the growth and development of tissues and organs of meat lambs. Chinese Herbivorous Animal Science, 2012(S1): 302-304. |
许贵善, 刁其玉, 邓凯东, 等. 限饲对肉用羔羊组织器官生长发育的影响. 中国草食动物科学, 2012(S1): 302-304. | |
28 | Zhou R, Liu L S, Wu J P, et al. Effects of oregano essential oil on nutrient degradation rate, fermentation characteristics and CH4 yield of sheep rumen in vitro. Acta Prataculturae Sinica, 2019, 28(11): 168-176. |
周瑞, 刘立山, 吴建平, 等. 牛至精油对绵羊瘤胃体外养分降解率、发酵特性及CH4产量的影响. 草业学报, 2019, 28(11): 168-176. | |
29 | Song R Y, Wang H R, Wang W, et al. Influence of diet with different nitrogen and energy sync hronous release on the ruminal fermentation in vitro and microbial protein synthesis in rumen of dairy cows. China Dairy Cattle, 2010(5): 711. |
30 | Bai D Y. Effects of dietary energy levels on fattening performance, rumen fermentation and nutrient metabolism of simmental hybrid bulls. Shijiazhuang: Hebei Agricultural University, 2019. |
白大洋. 日粮能量水平对西门塔尔杂交公牛育肥性能、瘤胃发酵及养分代谢的影响. 石家庄: 河北农业大学, 2019. | |
31 | National Research Council. Nutrient requirements of beef cattle 7th. Revised Edition Washington, D C: National Academy Press, 1996: 85-97. |
32 | Luo Y. Combination effect of mulberry leaves and Leymus chinensis and its effect on rumen fermentation, growth performance, fat deposition and meat quality in sheep. Yangzhou: Yangzhou University, 2018. |
罗阳.桑树叶与羊草的组合效应及其对绵羊瘤胃发酵、生长性能、脂肪沉积和肉品质的影响. 扬州: 扬州大学, 2018. | |
33 | Thao N T, Wanapat M, Cherdthong A, et al. Effects of eucalyptus crude oils supplementation on rumen fermentation, microorganism and nutrient digestibility in swamp buffaloes. Asian-Australasian Journal of Animal Sciences, 2014, 27(1): 46-54. |
34 | Zhao G Q, Jia Y H, Chen X L, et al. Effects of different NDF/NFE ratios on fermentation parameters of goat rumen. Chinese Livestock Magazine, 2006(13): 29-33. |
赵国琦, 贾亚红, 陈小连, 等. 不同NDF/NFE比的日粮对山羊瘤胃发酵参数影响的研究. 中国畜牧杂志, 2006(13): 29-33. | |
35 | Wang Y Y. Effects of dietary nutrient levels on the bacterial flora, pH and I VFA levelsiin rumen of Taan sheep. Yangling: Northwest Agriculture and Forestry University of Science and Technology, 2017. |
王尧悦. 日粮营养水平对滩羊瘤胃细菌区系及pH和VFA的影响. 咸阳: 西北农林科技大学, 2017. | |
36 | Wang G C, Yang J L, Wang H R, et al. Effects of nutritional restriction and compensation on rumen environment and serum urea nitrogen and creatinine in Mongolian lambs. Feed Industry, 2015, 36(19): 40-43. |
王桂超, 杨金丽, 王海荣, 等. 营养限制与补偿对蒙古羔羊瘤胃内环境及血清尿素氮和肌酐的影响. 饲料工业, 2015, 36(19): 40-43. | |
37 | Xia C Q. Effects of nutrient level on growth performance, blood biochemical indexes, rumen fermentation, slaughtering performance and meat quality of holstein milk bull. Beijing: China Agricultural University, 2018. |
夏传齐. 营养水平对荷斯坦奶公牛生长性能、血液生化指标、瘤胃发酵、屠宰性能及肉品质的影响. 北京: 中国农业大学, 2018. | |
38 | Sun H X. Study on changes of digestive enzyme activity in small intestine of Lamb. Shijiazhuang: Hebei Agricultural University, 2003. |
孙洪新. 羔羊小肠消化酶活性变化规律研究. 石家庄: 河北农业大学, 2003. | |
39 | Dong J J, Gao Y X, Li Y, et al. Effects of yeast polysaccharide on gastrointestinal development and digestive En. Chinese Journal of Animal Nutrition, 2018, 30(12): 5247-5254. |
董金金, 高艳霞, 李妍, 等. 酵母多糖对哺乳犊牛胃肠道发育及消化酶活性的影响. 动物营养学报, 2018, 30(12): 5247-5254. | |
40 | Jin X P, Peng Q H, Hu R, et al. Effects of cold season feeding on morphological development of small intestine and gene expression of nutrient transporter in Tibetan sheep. Journal of Animal Husbandry and Veterinary Medicine, 2017, 48(2): 260-271. |
景小平, 彭全辉, 胡瑞, 等. 冷季补饲对藏羊小肠形态发育及营养物质转运载体基因表达量的影响. 畜牧兽医学报, 2017, 48(2): 260-271. | |
41 | Chen J Q, Ding L M, Gao Q, et al. Effects of limited feeding and nutrition compensation on growth performance, digestion and metabolism, and rumen cellulase activity in small-tailed sheep. Chinese Journal of Animal Nutrition, 2015, 27(7): 2085-2093. |
陈军强, 丁路明, 高强, 等. 限饲与营养补偿对小尾寒羊生长性能、消化代谢和瘤胃液纤维素酶活性的影响. 动物营养学报, 2015, 27(7): 2085-2093. | |
42 | Jing X P, Wang Z S, Peng Q H, et al. Effect of cold season supplementary feeding on digestive tract enzyme activity and apparent digestibility of Tibetan sheep. Chinese Journal of Animal Nutrition, 2016, 28(8): 2404-2413. |
景小平, 王之盛, 彭全辉, 等. 冷季补饲对藏羊消化道消化酶活性及表观消化率的影响. 动物营养学报, 2016, 28(8): 2404-2413. | |
43 | Li L J, Cheng S R, Diao Q Y, et al. Effects of different NFC/NDF levels on rumen fermentation parameters and microbial flora diversity of calves. Journal of Animal Husbandry and Veterinary Medicine, 2017, 48(12): 2347-2357. |
李岚捷, 成述儒, 刁其玉, 等. 不同NFC/NDF水平饲粮对犊牛瘤胃发酵参数和微生物区系多样性的影响. 畜牧兽医学报, 2017, 48(12): 2347-2357. | |
44 | Koike S, Kobayashi Y. Fibrolytic rumen bacteria: Their ecology and functions. Asian-Australasian Journal of Animal Sciences, 2009, 22(1): 131-138. |
45 | Wang Y Y, Zhao Z Y, Wang X T, et al. The effects of dietary nutrient level on the pH and VFA contents of rumen-associated microbial flora in 150 and 180 days old Tan sheep. Journal of Animal Husbandry and Veterinary Medicine, 2016, 47(10): 2060-2070. |
王尧悦, 赵钊艳, 王兴涛, 等. 日粮营养水平对150~180日龄滩羊瘤胃相关微生物菌群数量、pH和VFA含量的影响. 畜牧兽医学报, 2016, 47(10): 2060-2070. | |
46 | Wang B X, Chen G J, Guo C H, et al. Effects of energy level on growth performance, slaughter performance, rumen fermentation parameters and number of rumen microorganisms. Animal Husbandry and Veterinary Medicine, 2017, 44(2): 469-475. |
王斌星, 陈光吉, 郭春华, 等. 能量水平对舍饲育肥牦牛生长性能、屠宰性能、瘤胃发酵参数和瘤胃微生物数量的影响. 中国畜牧兽医, 2017, 44(2): 469-475. | |
47 | Zhao Y X, Ao C J, Bao Z B, et al. Effects of scallions and their extracts on rumen fermentation and microbial flora of sheep. Chinese Journal of Animal Nutrition, 2019, 31(5): 2313-2322. |
赵亚星, 敖长金, 包志碧, 等. 沙葱及其提取物对肉羊瘤胃发酵及微生物区系的影响. 动物营养学报, 2019, 31(5): 2313-2322. | |
48 | Lu Y, Lin B, Wang T, et al. Effects of garlic oil on rumen fermentation, methane production and microbiota in vitro. Chinese Journal of Animal Nutrition, 2010, 22(2): 386-392. |
陆燕, 林波, 王恬, 等. 大蒜油对体外瘤胃发酵、甲烷生成和微生物区系的影响. 动物营养学报, 2010, 22(2): 386-392. | |
49 | Li D Y, Meng Q X, Ren L P, et al. Application of plant extract in ruminant breeding. Chinese Journal of Animal Nutrition, 2012, 24(11): 2085-2091. |
李德勇, 孟庆翔, 任丽萍, 等. 植物提取物在反刍动物饲养中的应用. 动物营养学报, 2012, 24(11): 2085-2091. | |
50 | Bird S H, Leng R A. Further studies on the effects of the presence or absence of protozoa in the rumen on live-weight gain and wool growth of sheep. British Journal of Nutrition, 1984, 52(3): 607-611. |
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