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草业学报 ›› 2020, Vol. 29 ›› Issue (12): 112-120.DOI: 10.11686/cyxb2020068

• 研究论文 • 上一篇    下一篇

枯草芽孢杆菌和3种放线菌对盐胁迫下鬼针草和鳢肠种子萌发及幼苗生长的影响

王桔红1(), 史生晶2, 陈文3(), 甘桂媚1, 陈赛娜1, 李张伟4   

  1. 1.韩山师范学院食品工程与生物科技学院,广东 潮州 521041
    2.西北师范大学生命科学学院,甘肃 兰州 730070
    3.韩山师范学院烹饪与酒店管理学院,广东 潮州 521041
    4.韩山师范学院化学与环境工程学院,广东 潮州 521041
  • 收稿日期:2020-02-21 修回日期:2020-03-23 出版日期:2020-12-28 发布日期:2020-12-28
  • 通讯作者: 陈文
  • 作者简介:Corresponding author. E-mail: cyw1018@sina.com
    王桔红(1963-), 女, 河北无极人,教授。E-mail: wjuh1918@163.com
  • 基金资助:
    国家自然科学基金项目(31770584);广东省科技计划项目(2017a030303086);潮州市科技计划项目(2017gy04)

Effects of Bacillus subtilis and three actinomycetes on seed germination and seedling growth of Bidens pilosa and Eclipta prostrata under salt stress

Ju-hong WANG1(), Sheng-jing SHI2, Wen CHEN3(), Gui-mei GAN1, Sai-na CHEN1, Zhang-wei LI4   

  1. 1.College of Food Technology and Life Science,Hanshan Normal University,Chaozhou 521041,China
    2.College of Life Sciences,Northwestern Normal University,Lanzhou 730070,China
    3.School of Culinary Arts and Hospitality Management,Hanshan Normal University,Chaozhou 521041,China
    4.College of Chemistry and Environmental Engineering,Hanshan Normal University,Chaozhou 521041,China
  • Received:2020-02-21 Revised:2020-03-23 Online:2020-12-28 Published:2020-12-28
  • Contact: Wen CHEN

摘要:

为探查枯草芽孢杆菌和放线菌对植物抗盐性的影响,采用培养皿法研究枯草芽孢杆菌和3种放线菌(粉孢类群2-5、淡紫灰类群6-3和烬灰类群1-6)对盐胁迫下鬼针草和鳢肠种子萌发及幼苗生长的影响。结果表明:1)枯草芽孢杆菌和3种放线菌在25~75 mmol·L-1盐浓度下提高鬼针草种子发芽率,而4种菌液对盐胁迫下鳢肠种子发芽率影响较小。2)枯草芽孢杆菌和3种放线菌对盐胁迫下鬼针草根长作用较小,对鳢肠根长具有不同程度的促生作用。3)4种菌液对盐胁迫下鬼针草茎长作用较小,对鳢肠茎长具有显著的促生作用,100 mmol·L-1盐浓度下鳢肠茎长分别增加了30.56%、28.57%、26.47%和24.24%。4)枯草芽孢杆菌和3种放线菌对盐胁迫下鬼针草干重影响较小,但能不同程度地提高鳢肠干重。枯草芽孢杆菌和3种放线菌对盐胁迫下鬼针草种子发芽率和鳢肠根长、茎长及干重具有较显著的促生作用,有利于鬼针草和鳢肠幼苗适应盐逆境。

关键词: 枯草芽孢杆菌, 放线菌, 盐胁迫, 种子萌发, 幼苗生长

Abstract:

This research investigated the effects of Bacillus subtilis and actinomycetes on salt tolerance of plants. Specifically, the effects of B. subtilis and three actinomycetes (Roseosporus 2-5, Lavendulae 6-3 and Cinereus 1-6) on seed germination and seedling growth of Bidens pilosa and Eclipta prostrata under salt stress were studied using a petri dish method. It was found that: 1) B. subtilis and the three actinomycetes increased the seed germination rate of B. pilosa at salt concentrations of 25-75 mmol·L-1, but the four microbial solutions had little effect on the seed germination rate of E. prostrata. 2) B. subtilis and the three actinomycetes had little effect on the root length of B. pilosa under salt stress, and had different degrees of growth promotion effect on the root length of E. prostrata. 3) The four microbial solutions had little effect on the stem length of B. pilosa, but significantly promoted intestinal stem growth of E. prostrata under salt stress, with B. subtilis and the 3 ascomycetes increasing the stem length of this species by 30.56%, 28.57%, 26.47% and 24.24%, respectively at 100 mmol·L-1 salt concentration. 4) Treatment with B. subtilis and the three actinomycetes at the various salt concentrations reduced the dry weight of B. pilosa, compared to seedlings germinated in a sterile solution, but the dry weight of E. prostrata was increased to different degrees in the same conditions. In summary, B. subtilis and the three actinomycetes had significant growth promotion effects on seed germination rate of B. pilosa and root length, stem length and dry weight of E. prostrata under salt stress, which was beneficial to adaptation of B. pilosa and E. prostrata seedlings to salt stress.

Key words: Bacillus subtilis, actinomycetes, salt stress, seed germination, seedling growth