草业学报 ›› 2026, Vol. 35 ›› Issue (1): 130-139.DOI: 10.11686/cyxb2025067
• 研究论文 • 上一篇
王若非(
), 李昕哲, 李祺策, 张嘉仪, 王焙钧, 谢文刚(
)
收稿日期:2025-03-04
修回日期:2025-04-15
出版日期:2026-01-20
发布日期:2025-11-13
通讯作者:
谢文刚
作者简介:E-mail: xiewg@lzu.edu.cn基金资助:
Ruo-fei WANG(
), Xin-zhe LI, Qi-ce LI, Jia-yi ZHANG, Bei-jun WANG, Wen-gang XIE(
)
Received:2025-03-04
Revised:2025-04-15
Online:2026-01-20
Published:2025-11-13
Contact:
Wen-gang XIE
摘要:
本研究以老芒麦为材料,旨在评估不同浓度(0、50、100、150和200 mg·L-1)纳米硅处理对其苗期抗寒性的影响。通过低温胁迫试验和纳米硅处理,发现在低温胁迫下纳米硅处理显著促进了老芒麦根系的伸长(P<0.05),而对株高无显著影响(P>0.05)。此外,对试验中各指标全面综合分析表明,和对照相比,200 mg·L-1纳米硅处理组老芒麦幼苗的根长、脯氨酸含量、叶绿素含量分别提高了63.2%、178.6%和15.6%,相对电导率、丙二醛含量和脱落酸含量分别降低了17.5%、3.88%和2.23%。综上,纳米硅可通过调节细胞渗透压、维持光合作用效率以及调节植物激素水平等维持植物在低温胁迫下能正常进行生理代谢,以增强植物的抗寒性。本研究为纳米硅在牧草抗寒育种中的应用提供了理论支撑。
王若非, 李昕哲, 李祺策, 张嘉仪, 王焙钧, 谢文刚. 不同浓度的硅处理对老芒麦苗期抗寒性的影响[J]. 草业学报, 2026, 35(1): 130-139.
Ruo-fei WANG, Xin-zhe LI, Qi-ce LI, Jia-yi ZHANG, Bei-jun WANG, Wen-gang XIE. Effects of different concentrations of silicon on the cold resistance of Elymus sibiricus seedlings[J]. Acta Prataculturae Sinica, 2026, 35(1): 130-139.
图1 不同浓度纳米硅处理对老芒麦幼苗株高的影响误差棒为标准差Error bars represent standard deviation (SD). T1~5: 0, 50, 100, 150, 200 mg·L-1纳米硅Nano silicon. NT: 常温组(22 ℃)Normal temperature group (22 ℃); LT: 低温组(4 ℃) Low temperature group (4 ℃). 下同The same below.
Fig.1 Effects of different concentrations of nano-silicon treatments on plant height of E. sibiricus seedlings
图2 不同浓度纳米硅处理对老芒麦幼苗根长的影响不同小写字母表示在常温处理下各组间有显著性差异(P<0.05);不同大写字母表示低温处理下各组间有差异显著(P<0.05)。Different lowercase letters indicate significant differences (P<0.05) among groups under normal temperature; Different uppercase letters denote significant differences (P<0.05) among groups under low temperature. 下同The same below.
Fig.2 Effects of different concentrations of nano-silicon treatments on root length of E. sibiricus seedlings
图3 不同浓度纳米硅处理对老芒麦幼苗相对电导率的影响
Fig.3 Effects of different concentrations of nano-silicon treatments on the relative conductivity of E. sibiricus seedlings
图7 不同浓度纳米硅处理对老芒麦幼苗超氧化物歧化酶活性的影响
Fig.7 Effects of different concentrations of nano-silicon treatments on superoxide dismutase activity of E. sibiricus seedlings
图8 不同浓度纳米硅处理对老芒麦幼苗脱落酸浓度的影响
Fig.8 Effects of different concentrations of nano-silicon treatments on abscisic acid concentration in E. sibiricus seedlings
图9 抗寒指标相关性分析*用以标识变量间存在显著相关性(P<0.05),而**则指示变量间呈极显著的相关性(P<0.01)。* was used to identify the significant correlation between variables (P<0.05),while ** indicated that there was a extremely significant correlation between variables (P<0.01).
Fig.9 Correlation analysis of cold tolerance related traits
处理 Treatment | 隶属函数值Subordinate function values | 综合评价值 Comprehensive evaluation value | 排序 Rank | |||||
|---|---|---|---|---|---|---|---|---|
相对电导率 Relative conductivity | 脯氨酸 Proline | 丙二醛 Malondialdehyde | 叶绿素 Chlorophyll | 超氧化物歧化酶 Superoxide dismutase | 脱落酸 Abscisic acid | |||
| T1 | 0.0129 | 0.0523 | 0.9451 | 0 | 0.2796 | 0.3893 | 0.2799 | 4 |
| T2 | 0 | 0.2471 | 0.5071 | 0.3419 | 0.3951 | 0.0912 | 0.2637 | 5 |
| T3 | 0.0776 | 0 | 0 | 1.0000 | 1.0000 | 0.6945 | 0.4620 | 3 |
| T4 | 1.0000 | 0.1629 | 0.6491 | 0.0110 | 0.1250 | 1.0000 | 0.4913 | 2 |
| T5 | 0.8463 | 1.0000 | 1.0000 | 0.3133 | 0 | 0 | 0.5266 | 1 |
表1 不同浓度纳米硅处理下苗期老芒麦抗寒性隶属函数值及综合评价
Table 1 Membership function value and comprehensive evaluation of cold resistance of E. sibiricus under different concentrations of nano-silicon treatments
处理 Treatment | 隶属函数值Subordinate function values | 综合评价值 Comprehensive evaluation value | 排序 Rank | |||||
|---|---|---|---|---|---|---|---|---|
相对电导率 Relative conductivity | 脯氨酸 Proline | 丙二醛 Malondialdehyde | 叶绿素 Chlorophyll | 超氧化物歧化酶 Superoxide dismutase | 脱落酸 Abscisic acid | |||
| T1 | 0.0129 | 0.0523 | 0.9451 | 0 | 0.2796 | 0.3893 | 0.2799 | 4 |
| T2 | 0 | 0.2471 | 0.5071 | 0.3419 | 0.3951 | 0.0912 | 0.2637 | 5 |
| T3 | 0.0776 | 0 | 0 | 1.0000 | 1.0000 | 0.6945 | 0.4620 | 3 |
| T4 | 1.0000 | 0.1629 | 0.6491 | 0.0110 | 0.1250 | 1.0000 | 0.4913 | 2 |
| T5 | 0.8463 | 1.0000 | 1.0000 | 0.3133 | 0 | 0 | 0.5266 | 1 |
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