影响冰草成熟胚组织培养再生体系频率的因素

草业学报 ›› 2009, Vol. 18 ›› Issue (1): 80-85.

影响冰草成熟胚组织培养再生体系频率的因素

徐春波¹,米福贵²,王勇²,李兴酉¹ ,赵海霞¹

1.中国农业科学院草原研究所,内蒙古呼和浩特010010;
2.内蒙古农业大学生态环境学院,内蒙古呼和浩特010018

收稿日期:2008-05-22 出版日期:2009-01-25 发布日期:2009-02-20
作者简介:徐春波(1979-),女,内蒙古扎兰屯人,硕士。E-mail:xcb972002@163.com
基金资助:
中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所)资助。

Factors influencing plant regeneration systems in mature embryo culture of Agropyron

XU Chun-bo¹, MI Fu-gui², WANG Yong², LI Xing-you¹, ZHAO Hai-xia¹

1.Grassland Research Institute of Chinese Academy of Agricultural Sciences, Huhhot 010010, China;
2.College of Ecology and Environment, Inner Mongolia Agricultural University, Huhhot 010018, China

Received:2008-05-22 Online:2009-01-25 Published:2009-02-20

摘要/Abstract

摘要： 对影响冰草成熟胚组培再生体系频率的2个因素——基因型和脱落酸(ABA)进行了研究,以期为当前冰草的基因转化提供最佳的再生体系。研究表明,在相同的培养条件下,4个基因型(品种)冰草成熟胚愈伤组织诱导能力和分化能力有明显的差异,4个基因型中以蒙古冰草成熟胚在愈伤组织诱导、分化等方面表现最佳,愈伤组织诱导率和分化率分别达到95％和72%,是遗传转化的良好受体材料;继代培养基中附加0.3mg/L脱落酸(ABA)可以明显改善冰草成熟胚愈伤组织状态,增加胚性愈伤组织率,从而提高分化率。

Abstract: The influence of both genotype and abscisic acid(ABA) on plant regeneration frequency of mature embryos of Agropyron was studied for establishing an effective system for gene transformation. Plant regeneration from mature embryos through direct callus induction was investigated in different species. There were differences in the capacity of callus induction and differentiation of mature embryos between different genotypes. There was suitable material for callus induction from mature embryos of A. mongolicum whose callus initiation frequency was 95% and callus differentiation frequency was 72%. The high frequency of embryogenic callus formation and differentiation were enhanced in MS solid medium with 0.3 mg/L ABA and the best genotype for genetic transformation was A. mongolicum.

中图分类号:

徐春波,米福贵,王勇,李兴酉 ,赵海霞. 影响冰草成熟胚组织培养再生体系频率的因素[J]. 草业学报, 2009, 18(1): 80-85.

XU Chun-bo, MI Fu-gui, WANG Yong, LI Xing-you, ZHAO Hai-xia. Factors influencing plant regeneration systems in mature embryo culture of Agropyron[J]. Acta Prataculturae Sinica, 2009, 18(1): 80-85.

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