老芒麦种质的醇溶蛋白遗传多样性研究

草业学报 ›› 2009, Vol. 18 ›› Issue (3): 59-66.

老芒麦种质的醇溶蛋白遗传多样性研究

马啸¹,陈仕勇¹,张新全^1*,周永红²,白史且³,刘伟¹

1.四川农业大学动物科技学院,四川雅安 625014;
2.四川农业大学小麦研究所,
四川成都 611130;
3.四川省草原科学研究院,四川成都 611731

收稿日期:2008-07-29 出版日期:2009-06-20 发布日期:2009-06-20
作者简介:马啸(1977-),男,山东济宁人,讲师,博士。E-mail:maroar@163.com
基金资助:
四川省“十一五”牧草育种攻关项目和国家重点基础研究发展计划(973计划)项目(2007CB108907)资助。

Genetic diversity of Gliadin in worldwide germplasm collections of Elymus sibiricus

MA Xiao¹, CHEN Shi-yong¹, ZHANG Xin-quan¹, ZHOU Yong-hong², BAI Shi-qie³, LIU Wei¹

1.The Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural
University, Ya’an 625014, China;
2.Triticeae Research Institute, Sichuan Agricultural University,
Chengdu 611130, China;
3.Sichuan Academy of Grassland Sciences, Chengdu 611731, China

Received:2008-07-29 Online:2009-06-20 Published:2009-06-20

摘要/Abstract

摘要： 利用A-PAGE的醇溶蛋白标记,对来自亚洲和北美的86份老芒麦种质的遗传多样性和遗传关系进行了分析。电泳共检测到52条醇溶蛋白条带,其中47条为多态性条带,多态性带百分比达90.4%。种质间遗传相似系数的变幅为0.108~0.952,平均值为0.373。利用Shannon多样性指数反映了类似的结果,即供试种质间的多样性指数达到0.460的较高水平。基于多样性指数计算了老芒麦种质地理类群遗传分化程度,地理类群内和类群间的遗传变异分别占总变异的55.8%和44.2%,这表明种质间存在较高水平的遗传多样性。对供试种质和地理类群的聚类分析结果均显示,来源于青藏高原的种质与其他地理来源的种质具有较大的差异,可以分成明显的2支。这种聚类结果可能与老芒麦种质的地理来源和生态适应性有关。本研究结果可为老芒麦种质的收集保护及核心种质构建提供有益信息。

Abstract: Gliadin markers based on A-PAGE were used to analyze genetic diversity and genetic relationships among eighty-six Elymus sibiricus accessions from Asia and North America. A total of 52 gliadin bands were scored, of which 47 were polymorphic (90.4%). The generated similarity matrix showed that the genetic diversity within the accessions was fairly high (average similarity index=0.373). Similarity values among the accessions ranged from 0.108 and 0.952. Similar results were obtained when genetic diversity was estimated using the Shannon index of diversity. The total genetic diversity across all accessions was 0.460. The proportion of variation explained by within geographic groups and between geographic groups diversity was 0.559 and 0.442, respectively. Cluster analysis showed a clear demarcation between accessions from the Qinghai-Tibetan Plateau, China and the others as separate groups. The extent of genetic diversity and genetic relationship among accessions is discussed. The clustering pattern probably depended on geographic origin and ecological adaptability of the accessions.

中图分类号:

马啸,陈仕勇,张新全,周永红,白史且,刘伟. 老芒麦种质的醇溶蛋白遗传多样性研究[J]. 草业学报, 2009, 18(3): 59-66.

MA Xiao, CHEN Shi-yong, ZHANG Xin-quan, ZHOU Yong-hong, BAI Shi-qie, LIU Wei. Genetic diversity of Gliadin in worldwide germplasm collections of Elymus sibiricus[J]. Acta Prataculturae Sinica, 2009, 18(3): 59-66.

参考文献

[1]Dewey D R. The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae[A[. In: Gustafson J P. Gene Manipulation in Plant Improvement[C[. New York: Plenum Press, 1984. 209-280.
[2]Dewey D R. Cytogenetics of Elymus sibiricus and its hybrids with Agropyron tauri, Elymus canadensis, and Agropyron caninu[J[. Botanical Gazette, 1974, 135: 80-87.
[3]Bowden W M, Cody W J. Recognition of Elymus sibiricus L. from Alaska and the district of Mackenzie[J[. Bulletin of the Torrey Botanical Club, 1961, 88: 153-155.
[4]蒋尤泉.中国作物及其近缘野生植物——饲用及绿肥作物卷[M[. 北京: 中国农业出版社, 2007. 139-141.
[5]Lu B R. Biosystematic investigations of Asiatic wheatgrasses - Elymus L. (Triticeae: Poaceae)[D[. Svalv, Sweden: Swedish University of Agricultural Sciences, 1993.
[6]盘朝邦, 王元富, 曾宁. 栽培老芒麦水热生态型分化初探[J[. 中国草地学报, 1992, 14(5): 58-63.
[7]梁宇, 刁亚瑞, 刘公社, 等. 中国东北羊草自然种群的扩增片段长度多态性研究[J[. 草业学报, 2007, 16(2): 124-134.
[8]范彦, 李芳, 张新全, 等. 扁穗牛鞭草种质遗传多样性的ISSR分析[J[. 草业学报, 2007, 16(4): 76-81.
[9]Díaz O, Salomon B, von Bothmer R. Genetic diversity and structure in populations of Elymus caninus (L.) (Poaceae)[J[. Hereditas, 1999, 131: 63-74.
[10]袁庆华, 张吉宇, 张文淑, 等. 披碱草和老芒麦野生居群生物多样性研究[J[. 草业学报, 2003, 12(5): 44-49.
[11]鄢家俊, 白史且, 马啸, 等. 川西北高原野生老芒麦居群穗部形态多样性研究[J[. 草业学报, 2007, 16(6): 99-106.
[12]Kostina E V, Agafonov A V, Salomon B. Electrophoretic properties and variability of endosperm proteins of Elymus caninus (L.)[A[. In: Jaradat A A. Proceedings of the 3^rd International Tritiecae Symposium, Aleppo, Syria[C[. Enfield, New Hampshire, USA: Science Publishers, Inc., 1998. 265-272.
[13]Agafonov A V, Baum B R. Variation of endosperm proteins in the complex of Elymus trachycaulus (Link) Gould ex Shinners[A[. In: Jaradat A A. Proceedings of the 3^rd International Tritiecae Symposium, Aleppo, Syria[C[. Enfield, New Hampshire, USA: Science Publishers, Inc., 1998. 273-282.
[14]马啸, 周永红, 于海清, 等. 野生垂穗披碱草种质的醇溶蛋白遗传多样性分析[J[. 遗传, 2006, 28(6): 699-706.
[15]杨烈, 张新全, 杨武云, 等. 四川青衣江、岷江流域鹅观草遗传多样性的溶蛋白分析[J[. 草业学报, 2006, 15(4): 12-20.
[16]车永和, 杨欣明, 杨燕萍, 等. 沙生冰草醇溶蛋白遗传多样性研究[J[. 麦类作物学报, 2007, 27(5): 767-771.
[17]兰秀锦, 郑有良, 刘登才, 等. 山羊草属植物醇溶蛋白的遗传多样性分析[J[. 草业学报, 2006, 15(6): 93-100.
[18]杨瑞武, 魏秀华, 周永红, 等. 赖草属植物醇溶蛋白的遗传多态性[J[. 云南植物研究, 2004, 26(1): 103-110.
[19]Draper S R. ISTA variety committee report of the working group for biochemical tests for cultivar identification 1983-1986[J[. Seed Science and Technology, 1987, 15: 431-434.
[20]Bushuk W, Zillman R R. Wheat cultivars identification by gliadin electrophoregrams. I. Apparatus method and nomenclature[J[. Canadian Journal of Plant Science, 1978, 58: 505-515.
[21]Yeh F C, Yang R C, Boyle T, et al. POPGENE, the User Friendly Shareware for Population Genetic Analysis[M[. Edmonton, Canada: Molecular Biology and Biotechnology Center, University of Alberta, 1997.
[22]Lewontin R C. The apportionment of human diversity[A[. In: Dobzhansky T H, Hecht M K, Steere W C. Evolutionary Biology(vol 6)M[. New York: Appleton-Century-Crofts, 1972. 381-398.
[23]Wachira F N, Waugh R, Hachett C A, et al. Detection of genetic diversity in tea (Camellia sinesis) using RAPD markers[J[. Genome, 1995, 38: 201-210.
[24]Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases[J[. Proceedings of the National Academy of Sciences of the United States of America, 1979, 76: 5269-5273.
[25]Mantel N. The detection of disease clustering and a generalized regression approach[J[. Cancer Research, 1967, 27: 209-220.
[26]Alvarez J B, Moral A, Martin L M. Polymorphism and genetic diversity for the seed storage proteins in Spanish cultivated einkorn wheat (Triticum monococcum L. ssp. monococcum)[J[. Genetic Resources and Crop Evolution, 2006, 53: 1061-1067.
[27]Ciaffi M, Lafiandra D, Porceddu E, et al. Storage-protein variation in wild emmer (Triticum turgidum ssp. dicoccoides) II. Patterns of allele distribution[J[. Theoretical and Applied Genetics, 1993, 86: 518-525.
[28]Godt M J W, Johnson B R, Hamrick J L. Genetic diversity and population size in four rare southern Appalachian plant species[J[. Conservation Biology, 1996, 10: 796-806.
[29]Sun M. Effects of population size, mating system, and evolutionary origin on genetic diversity in Spiranthes sinensis and S. hongkongensis[J[. Conservation Biology, 1996, 10: 785-795.
[30]Gaudett M, Salomon B, Sun G L. Molecular variation and population structure in Elymus trachycaulus and comparison with its morphologically similar E. alaskanus[J[. Plant Systematics and Evolution, 2005, 250: 81-91.
[31]Johnson R C, Johnston W J, Golob C T, et al. Characterization of the USDA Poa pratensis collection using RAPD markers and agronomic descriptors[J[. Genetic Resources and Crop Evolution, 2002, 49: 349-361.
[32]张镱锂, 李炳元, 郑度.论青藏高原范围与面积[J[. 地理研究, 2002, 21(1): 1-8.