不同品种紫花苜蓿酚酸类化感物质含量的研究

草业学报 ›› 2010, Vol. 19 ›› Issue (2): 183-189.

不同品种紫花苜蓿酚酸类化感物质含量的研究

李志华,沈益新,刘信宝,于静,王呼德呼,林仲安

南京农业大学动物科技学院,江苏南京 210095

收稿日期:2009-04-02 出版日期:2010-02-25 发布日期:2010-04-20
作者简介:李志华(1966-),女,内蒙古赤峰人,副教授,博士。E-mail lizhihua@njau.edu.cn
基金资助:
国家公益性行业(农业)科研专项经费项目(NYHYZX07-22)和国家大学生创新性实验计划项目(GJ0715)资助。

A study on the contents of allelochemical phenolic acids from different cultivars of alfalfa

LI Zhi-hua, SHEN Yi-xin, LIU Xin-bao, YU Jing, WANG Hu-de-hu, LIN Zhong-an

Collage of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, China

Received:2009-04-02 Online:2010-02-25 Published:2010-04-20

摘要/Abstract

摘要： 采用高效液相色谱法对10个品种紫花苜蓿地上部和根水浸提液中的绿原酸、香豆酸、羟基苯甲酸、咖啡酸和阿魏酸5种酚酸类化感物质的含量进行分析。研究表明,无论是地上部还是根水浸提液中,不同品种的紫花苜蓿酚酸类化感物质的含量存在差异,艾丽丝地上部和根水浸提液中绿原酸的含量分别为94.72和23.98μg/g,咖啡酸含量分别为30.49和6.33μg/g,与其他品种相比均较高;艾格地上部和根水浸提液阿魏酸含量分别为48.97和6.75μg/g,均明显高于其他品种。紫花苜蓿地上部和根水浸提液羟基苯甲酸含量分别与咖啡酸、绿原酸、阿魏酸含量呈极显著的正相关。紫花苜蓿地上部水浸提液中,香豆酸含量分别与咖啡酸和阿魏酸含量呈极显著的正相关,羟基苯甲酸与香豆酸呈显著正相关;根水浸提液中,咖啡酸含量分别与绿原酸和阿魏酸含量、绿原酸含量与阿魏酸含量呈显著正相关。另外,同一品种紫花苜蓿地上部与根水浸提液中同一种酚酸类含量存在差异。

Abstract: The contents of five allelochemical phenolic acids (chlorogenic acid, p-coumaric acid, p-hydroxybenzoic acid, caffeic acid, and ferulic acid ) from ten cultivars of alfalfa were analyzed by high performance liquid chromatography (HPLC). The allelochemical phenolic acids were aqueous extracts of aerial parts and roots and they differed between the alfalfa cultivars. The chlorogenic acid and caffeic acid contents of the extracts from the cultivar Alize were higher than those from other alfalfa cultivars. The chlorogenic acid contents of the extracts from aerial parts and roots of the cultivar Alize were 94.72 and 23.98 μg/g respectively and of caffeic acid were 30.49 and 6.3 3 μg/g respectively. The ferulic acid contents of extracts of aerial part and roots from the cultivar Alegro were 48.97 and 6.75 μg/g respectively, higher than those from other alfalfa cultivars. The p-hydroxybenzoic acid contents of the alfalfa extracts showed a highly significant positive correlation with the contents of caffeic acid, chlorogenic acid, and ferulic acid. The p-coumaric acid content of extracts from the aerial parts of alfalfa showed a highly significant positive correlation with the contents of caffeic acid and of ferulic acid, while the p-hydroxybenzoic acid content showed a highly significant positive correlation with the p-coumaric acid content. The caffeic acid content of the aqueous extracts of alfalfa roots showed a highly significant positive correlation with the chlorogenic acid and ferulic acid contents, and the chlorogenic acid content showed a highly significant positive correlation with the ferulic acid content. In addition, the phenolic acid content of the aqueous extracts of the same alfalfa cultivar differed between aerial parts and roots.

中图分类号:

李志华,沈益新,刘信宝,于静,王呼德呼,林仲安. 不同品种紫花苜蓿酚酸类化感物质含量的研究[J]. 草业学报, 2010, 19(2): 183-189.

LI Zhi-hua, SHEN Yi-xin, LIU Xin-bao, YU Jing, WANG Hu-de-hu, LIN Zhong-an. A study on the contents of allelochemical phenolic acids from different cultivars of alfalfa[J]. Acta Prataculturae Sinica, 2010, 19(2): 183-189.

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