γ射线对沟叶结缕草愈伤组织再生和耐盐性的影响

摘要/Abstract

摘要： 以沟叶结缕草匍匐茎诱导的胚性愈伤组织为材料,分别进行0Gy(对照)和10Gy的⁶⁰Coγ射线辐照处理,比较愈伤组织的再生能力以及再生植株的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性和脯氨酸含量,并对辐射材料进行不同浓度(0%,0.5%,1.0%,1.5%和2.0%)的NaCl筛选。结果表明,10Gy⁶⁰Coγ射线处理对愈伤组织的再生分化有显著的促进作用,分化形成3个以上小苗的外植体比率提高11.25%,但是愈伤组织再生率下降8.75%;再生植株的CAT活性提高16.02%,脯氨酸含量提高1.20倍;同对照相比,10Gyγ射线处理的愈伤组织获得的再生植株中SOD和POD活性没有显著变化。10Gy⁶⁰Coγ射线辐射处理能显著提高愈伤组织对NaCl的抗性;外植体的生长速度和存活率随着NaCl浓度的提高而下降;愈伤组织经过10Gy⁶⁰Coγ射线处理后,在含1.0%NaCl的再生培养基上获得最多的耐盐植株(成苗率为9.60%),这些植株转移到含1.0%NaCl的生根培养基上后保持旺盛生长。

Abstract: Embryogenic calluses induced from stolons of Manilagrass (Zoysia matrelia) were treated with ⁶⁰Co γ-irradiation at doses of 0 Gy (control) and 10 Gy. Regeneration of the callus, activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) enzymes, and proline content in the regenerated plants were measured. The treated materials were screened on MS basal media with different concentrations of NaCl (0%, 0.5%, 1.0%, 1.5%, and 2.0%). The results showed that 10 Gy ⁶⁰Co γ-irradiation had a significant promoting effect on callus regeneration and differentiation, and that the percentage of explants that formed more than 3 plantlets per explant was increased by 11.25%. However, the regeneration rate of the callus was reduced by 8.75%. 10 Gy ⁶⁰Co γ-irradiation of regenerated plants resulted in increases of CAT activity and proline content by 16.02% and 1.20 times, respectively. There were no significant changes in the activities of SOD and POD enzymes in the regenerated plants from calluses treated with 10 Gy γ-irradiation, compared with that of the controls. The maximum number of regenerated plants were obtained from calluses treated with 10Gy ⁶⁰Co γ-irradiation and grown on medium with 1.0% NaCl - 9.60% of the calluses formed plantlets. Regenerated plants maintained vigorous growth after transfer to rooting medium with 1.0% NaCl.

中图分类号:

贾玉芳,陈曙,柴明良. γ射线对沟叶结缕草愈伤组织再生和耐盐性的影响[J]. 草业学报, 2010, 19(5): 25-30.

JIA Yu-fang, CHEN Shu, CHAI Ming-liang. Effect of γ-irradiation on callus regeneration and salt tolerance in Manilagrass[J]. Acta Prataculturae Sinica, 2010, 19(5): 25-30.

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