野生马蹄金种质遗传多样性的SRAP研究

摘要/Abstract

摘要： 为评价野生马蹄金种质的遗传多样性,从80对SRAP引物中筛选出19对多态性高、稳定性好的引物,用于研究21份马蹄金材料,获得如下结果:1)19对引物共扩增出212条带,平均每对引物扩增出11.1条,其中多态性条带156条,平均每对引物8.2条,多态率为73.58%。材料间遗传相似系数变化范围为0.444~0.980,平均值为0.7112。结果表明,供试马蹄金材料具有较为丰富的遗传多样性。2)对所有材料的聚类分析发现,在GS值为0.78处所有供试材料可聚为3个类群,大部分来源地相同或生态环境相似的材料聚为一类,表明供试材料的聚类和生态地理环境具有一定的相关性。

Abstract: The genetic diversity of 21 Dichondra repens, were analyzed using 19 highly polymorphic and stable primer pairs selected from 80 pairs of SRAP primers and the 19 primer pairs produced 212 bands, including 156 polymorphic bands (8.2 bands per primer pair). The average percentage of polymorphic bands was 73.58%. Genetic similarity of D. repens was calculated by Nei’s method and showed that the genetic similarity ranged from 0.444-0.980, and the average Nei’s coefficient was 0.76. It suggested that there was a rich genetic diversity among the resources of D. repens tested. The shape of the SRAP dendrogram showed that the 21 materials could be divided into three groups with 0.78 thresholds, and materials from the same origin frequently clustered into the same group. The research indicated a correlation among the wild resources and their geographical and ecological environments.

中图分类号:

Q943

付薇,干友民,吴佳海,王小利,陈伟,张建波. 野生马蹄金种质遗传多样性的SRAP研究[J]. 草业学报, 2010, 19(6): 164-170.

FU Wei, GAN You-min, WU Jia-hai, WANG Xiao-li, CHEN Wei, ZHANG Jian-bo. Genetic diversity of Dichondra repens germplasm in southwest China by SRAP[J]. Acta Prataculturae Sinica, 2010, 19(6): 164-170.

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