沿阶草不同叶片对土壤铅胁迫的生理生化响应

摘要/Abstract

摘要： 为了解城市园林地被植物对日益增长的Pb污染的生理生化响应,采用盆栽控制实验,研究了Pb胁迫对沿阶草新生叶片(freshly leaf, FL)、成熟叶片(mature leaf, ML)和老叶叶片(old leaf, OL)超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性以及游离脯氨酸(Pro)和丙二醛(MDA)含量的影响。结果表明,Pb胁迫对沿阶草不同叶片SOD、POD、CAT活性以及游离Pro和MDA含量均有不同程度的影响。在设计的土壤Pb浓度下,沿阶草不同叶片SOD、POD和CAT活性随着土壤Pb浓度的增加表现出先增加后降低的趋势,游离Pro和MDA含量则随着土壤Pb浓度增加而增加,但不同叶片对土壤Pb胁迫的响应存在差异,以FL最敏感,ML次之,OL最不敏感。这表明,沿阶草能适应一定浓度范围的Pb污染胁迫,但不同叶片对Pb污染的响应与适应机制存在差异,FL对Pb胁迫的生理响应更敏感,而OL对Pb污染胁迫的敏感度相对较低。

Abstract: Ophiopogon bodinieri is an important turfgrass and ornamental cover plants which is widely used in urban garden construction in southern China. To understand the physiological & biochemical responses of different leaves of O. bodinieri to increasing Pb contamination, the effects of different soil Pb concentrations on the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and on the concentrations of free proline (Pro) and malondialdehyde (MDA) in different leaves were studied in a pot experiment with different Pb treatments. The activities of SOD, POD and CAT and the concentrations of Pro and MDA in fresh leaf (FL), mature leaf (ML), and old leaf (OL) of O. bodinieri were measured after Pb-exposure for 7 days. The activities of SOD, POD, and CAT increased at lower Pb concentrations, but decreased at higher ones, depending on the leaves and enzymes. Meanwhile, Pro and MDA concentrations in FL, ML and OL increased with increased soil Pb. The responses of different leaves of O. bodinieri to soil Pb stress differed. FL had higher activities of the antioxidative enzymes SOD, POD and CAT in comparison with the OL under the levels of soil Pb contamination tested, while the OL had higher concentrations of osmotic substances (Pro and MDA) in comparison with the FL and ML. O. bodinieri could adapt to a certain concentration range of soil Pb stress, and the response of fresh O. bodinieri leaves to soil Pb stress was more sensitive than that of old O. bodinieri leaves. The tolerance of old O. bodinieri leaves to soil Pb stress was stronger than those of fresh and mature leaves.

中图分类号:

刘碧英,潘远智,赵杨迪. 沿阶草不同叶片对土壤铅胁迫的生理生化响应[J]. 草业学报, 2011, 20(4): 123-128.

LIU Bi-ying, PAN Yuan-zhi, ZHAO Yang-di. Physiological & biochemical responses of different leaves of Ophiopogon bodinieri to soil lead stress[J]. Acta Prataculturae Sinica, 2011, 20(4): 123-128.

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