羊草乙醛脱氢酶基因LcALDH的克隆与表达分析

草业学报 ›› 2011, Vol. 20 ›› Issue (4): 187-193.

羊草乙醛脱氢酶基因LcALDH的克隆与表达分析

李新玲,吴姝菊^*,王全伟

哈尔滨师范大学生命科学与技术学院,黑龙江哈尔滨 150025

收稿日期:2011-01-20 出版日期:2011-04-25 发布日期:2011-08-20
通讯作者: E-mail:shuju1965@yahoo.com.cn
作者简介:李新玲(1975-),女,黑龙江龙江人,副教授。 E-mail:lixinling2002@126.com
基金资助:
哈尔滨市青年科技创新人才基金(2009RFQXN100)和哈尔滨师范大学博士启动基金资助。

Cloning and expression analysis of aldehyde dehydrogenase gene LcALDH from Leymus chinensis

LI Xin-ling, WU Shu-ju, WANG Quan-wei

College of Life Sciences and Technology, Harbin Normal University, Harbin 150025, China

Received:2011-01-20 Online:2011-04-25 Published:2011-08-20

摘要/Abstract

摘要： 采用RACE技术从羊草中克隆了乙醛脱氢酶基因LcALDH的cDNA(GenBank登录号EF492045)。长为1 712 bp的LcALDH cDNA序列含有编码500个氨基酸的1 503 bp的开放读码框、66 bp的5′非翻译区和144 bp的3′非翻译区。氨基酸序列中含有醛脱氢酶家族绝对保守的谷氨酸活性位点和半胱氨酸残基活性位点。分析LcALDH基因在不同胁迫条件下的表达情况,结果表明,LcALDH的表达受到低温、干旱、高盐和ABA的正调控作用。研究结果为进一步从分子水平探明羊草的抗逆机制,挖掘并利用植物抗逆基因奠定基础。

Abstract: In this research, cDNA(GenBank No. EF492045) of aldehyde dehydrogenase gene LcALDH was isolated from Leymus chinensis by RACE technology.The nucleotide sequence of LcALDH cDNA is 1 712 bp containing an open reading frame of 1 503 bp encoding 500 amino acids, with a 5′untranslated region of 66 bp and a 3′untranslated region of 144 bp. The amino acid sequence contains absolutely conservative glutamic acid and cysteine residues active sites of aldehyde dehydrogenase family. The analysis of LcALDH gene expression under different stress condition showed that LcALDH expression was positively regulated by low-temperature, drought, high salt and ABA. The research results will lay the foundation for both the further exploration of the stress resistant mechanism of L. chinensis from molecular level, and the utilization of plant gene.

中图分类号:

李新玲,吴姝菊,王全伟. 羊草乙醛脱氢酶基因LcALDH的克隆与表达分析[J]. 草业学报, 2011, 20(4): 187-193.

LI Xin-ling, WU Shu-ju, WANG Quan-wei. Cloning and expression analysis of aldehyde dehydrogenase gene LcALDH from Leymus chinensis[J]. Acta Prataculturae Sinica, 2011, 20(4): 187-193.

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