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草业学报 ›› 2011, Vol. 20 ›› Issue (4): 261-269.

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细胞工程技术在芒属能源作物上的应用

刘明稀1,蒋建雄2,易自力2*   

  1. 1.湖南农业大学农学院,湖南 长沙 410128;
    2.湖南农业大学生物科学技术学院,湖南 长沙 410128
  • 收稿日期:2010-05-20 出版日期:2011-04-25 发布日期:2011-08-20
  • 通讯作者: E-mail:yizili889@163.com
  • 作者简介:刘明稀(1975-),男,湖南新化人,讲师,博士。E-mail:lmx751221@163.com
  • 基金资助:
    国家自然科学基金项目(30971832)资助。

Application of cell engineering in improving Miscanthus as a dedicated bioenergy crop

LIU Ming-xi1, JIANG Jian-xiong2, YI Zi-li2   

  1. 1.College of Agronomy, Hunan Agricultural University, Changsha 410128, China;
    2.College of Biology and Technology, Hunan Agricultural University, Changsha 410128, China
  • Received:2010-05-20 Online:2011-04-25 Published:2011-08-20

摘要: 生物质能源是可再生能源的重要领域。芒属植物是极具开发潜力的生物质能源作物,欧美多国以奇岗(Miscanthus×Giganteus)作为芒属能源作物的代表对其进行了系统广泛的研究。本研究对细胞工程技术在芒属能源作物上的应用进行了总结: 1)离体再生体系建立的相关研究,主要集中于再生体系的愈伤诱导及分化增殖,研究内容涵盖了对组织培养影响重大的主要因素,包括激素、外植体、辅助添加物、培养基成分,甚至碳源的不同灭菌方式等; 2)染色体倍性操作,主要是染色体加倍研究,单倍体育种也有所突破。本研究最后对该领域的发展趋势进行了简要探讨。

Abstract: Biomass energy is an important renewable energy source and Miscanthus is considered as one of the most promising biomass energy crops. The research has been funded in the European Union and America for Miscanthus, especially the triploid species Miscanthus×Giganteus. The application of cell engineering in improving Miscanthus as a dedicated bioenergy crop is summarized as follows: 1) Studies of in vitro regeneration systems for Miscanthus, focusing on callus induction and differentiation or shoot proliferation, in which the main factors influencing regeneration efficiency were evaluated, including hormones, explants, supplementary additions, medium compositions, different carbon sources and their sterilization methods; 2) Studies of chromosome ploidy manipulation for Miscanthus, mostly for in vitro chromosome doubling of Miscanthus but one for haploid breeding. At the end of this paper the developing trends of cell engineering for the energy crop Miscanthus are briefly discussed.

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